Hydrogen peroxide stimulates cell motile activity through LPA receptor-3 in liver epithelial WB-F344 cells

Shibata, Ayano; Tanabe, Eriko; Inoue, Serina; Kitayoshi, Misaho; Okimoto, Souta; Hirane, Miku; Araki, Mutsumi; Fukushima, Nobuyuki; Tsujiuchi, Toshifumi

doi:10.1016/j.bbrc.2013.02.100

Cited by 16 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the reduction in migration that is mediated by p62 can be rescued by the knockdown of NQO1, this further supports a role for the miR-372 -p62-NQO1 cascade in the progression of HNSCC. Various lines of evidence support the hypothesis that ROS may participate in the regulation of Rho family members and modulate cytoskeletal organization or that ROS may activate other signals to increase migration [41, 48]. NQO1 was reported to attenuate ROS and the proliferation of vascular endothelial cells [49].…”

Section: Discussionmentioning

confidence: 99%

“…However, p62 is also able to modulate ROS through mTOR pathway, which bypasses the requirement of NQO1, in stromal fibroblast [36]. Multiple molecular mechanisms are known to take part in regulating cancer cell migration [1-3, 12, 15, 37-41]. In this study, we provide novel clues as to how miR-372 targets p62, which, in turn, enhances the mobility of HNSCC cells.…”

Section: Introductionmentioning

confidence: 86%

See 1 more Smart Citation

miR-372inhibits p62 in head and neck squamous cell carcinomain vitroandin vivo

Yeh¹,

Liu

Wong

et al. 2015

Oncotarget

View full text Add to dashboard Cite

Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

miR-372inhibits p62 in head and neck squamous cell carcinomain vitroandin vivo

Yeh¹,

Liu

Wong

et al. 2015

Oncotarget

View full text Add to dashboard Cite

show abstract

“…Scratch assays [ 7 – 10 ], also known as scrape or wound-healing assays [ 9 , 10 ], are routinely used to investigate the motion of cell fronts by creating a scratch in a cell monolayer and observing the motion of the cell front. Images of the front are captured over a period of time that is typically less than 24 h [ 7 , 11 , 12 ]. Use of short-time-scale experimental data is very common because it avoids the need for replenishing the nutrients in the assay.…”

Section: Introductionmentioning

confidence: 99%

How much information can be obtained from tracking the position of the leading edge in a scratch assay?

Johnston

Simpson

McElwain

2014

J. R. Soc. Interface.

View full text Add to dashboard Cite

Moving cell fronts are an essential feature of wound healing, development and disease. The rate at which a cell front moves is driven, in part, by the cell motility, quantified in terms of the cell diffusivity D, and the cell proliferation rate λ. Scratch assays are a commonly reported procedure used to investigate the motion of cell fronts where an initial cell monolayer is scratched, and the motion of the front is monitored over a short period of time, often less than 24 h. The simplest way of quantifying a scratch assay is to monitor the progression of the leading edge. Use of leading edge data is very convenient because, unlike other methods, it is non-destructive and does not require labelling, tracking or counting individual cells among the population. In this work, we study short-time leading edge data in a scratch assay using a discrete mathematical model and automated image analysis with the aim of investigating whether such data allow us to reliably identify D and λ. Using a naive calibration approach where we simply scan the relevant region of the (D, λ) parameter space, we show that there are many choices of D and λ for which our model produces indistinguishable short-time leading edge data. Therefore, without due care, it is impossible to estimate D and λ from this kind of data. To address this, we present a modified approach accounting for the fact that cell motility occurs over a much shorter time scale than proliferation. Using this information, we divide the duration of the experiment into two periods, and we estimate D using data from the first period, whereas we estimate λ using data from the second period. We confirm the accuracy of our approach using in silico data and a new set of in vitro data, which shows that our method recovers estimates of D and λ that are consistent with previously reported values except that that our approach is fast, inexpensive, non-destructive and avoids the need for cell labelling and cell counting.

show abstract

“…Cells are placed on a culture dish and incubated, eventually forming a monolayer . An artificial wound is created and images of the cell spreading by migration and proliferation are captured over 12–24 hours . Various cell types have been analysed for migration with this assay including epithelial and mesenchymal cancer cells, keratinocytes, normal epithelial cells, endothelial cells, and fibroblasts .…”

Section: Resultsmentioning

confidence: 99%

“…63 An artificial wound is created and images of the cell spreading by migration and proliferation are captured over 12-24 hours. [64][65][66] Various cell types have been analysed for migration with this assay including epithelial and mesenchymal cancer cells, keratinocytes, normal epithelial cells, endothelial cells, and fibroblasts. [67][68][69][70][71][72] The majority of scratch assays fall into one of two categories; wound assay or scrape assay.…”

Section: In Vitro Modelsmentioning

confidence: 99%

Non‐animal models of wound healing in cutaneous repair: In silico, in vitro, ex vivo, and in vivo models of wounds and scars in human skin

Uddin

Bayat

2017

Wound Repair Regeneration

View full text Add to dashboard Cite

Tissue repair models are essential to explore the pathogenesis of wound healing and scar formation, identify new drug targets/biomarkers and to test new therapeutics. However, no animal model is an exact replicate of the clinical situation in man as in addition to differences in the healing of animal skin; the response to novel therapeutics can be variable when compared to human skin. The aim of this review is to evaluate currently available non-animal wound repair models in human skin, including: in silico, in vitro, ex vivo, and in vivo. The appropriate use of these models is extremely relevant to wound-healing research as it enables improved understanding of the basic mechanisms present in the wound healing cascade and aid in discovering better means to regulate them for enhanced healing or prevention of abnormal scarring. The advantage of in silico models is that they can be used as a first in virtue screening tool to predict the effect of a drug/stimulus on cells/tissues and help plan experimental research/clinical trial studies but remain theoretical until validated. In vitro models allow direct quantitative examination of an effect on specific cell types alone without incorporating other tissue-matrix components, which limits their utility. Ex vivo models enable immediate and short-term evaluation of a particular effect on cells and its surrounding tissue components compared with in vivo models that provide direct analysis of a stimulus in the living human subject before/during/after exposure to a stimulus. Despite clear advantages, there remains a lack of standardisation in design, evaluation and follow-up, for acute/chronic wounds and scars in all models. In conclusion, ideal models of wound healing research are desirable and should mimic not only the structure but also the cellular and molecular interactions, of wound types in human skin. Future models may also include organ/skin-on-a-chip with potential application in wound healing research.

show abstract

Hydrogen peroxide stimulates cell motile activity through LPA receptor-3 in liver epithelial WB-F344 cells

Cited by 16 publications

References 20 publications

miR-372inhibits p62 in head and neck squamous cell carcinomain vitroandin vivo

miR-372inhibits p62 in head and neck squamous cell carcinomain vitroandin vivo

How much information can be obtained from tracking the position of the leading edge in a scratch assay?

Non‐animal models of wound healing in cutaneous repair: In silico, in vitro, ex vivo, and in vivo models of wounds and scars in human skin

Contact Info

Product

Resources

About