Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate

Bignucolo, Adam; Appanna, Vasu D.; Thomas, Sean C.; Auger, Christopher; Han, Sungwon; Omri, Abdelwahab

doi:10.1016/j.jbiotec.2013.07.002

Cited by 47 publications

(19 citation statements)

References 25 publications

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“…Some recent results have shown that pyruvate secretion and TCA cycle deregulation are crucial for pathogenicity in some bacteria [116], which is consistent with our findings [117]. However, in Xac such connections have not been shown until now.…”

Section: Resultssupporting

confidence: 93%

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

et al. 2017

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Background Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection.ResultsFor that, a 2D–based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants` Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants.ConclusionsThis is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1063-x) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 93%

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

et al. 2017

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“…PK has three functional cysteines susceptible to oxidization, Cys 358 essential for catalysis, and Cys 31 and Cys 424 involved in subunit interface interaction . Overexpression of PK can compensate for various inactivating reactions and elevated pyruvate production in Pseudomonas fluorescens was part of adaptation to H 2 O 2 stress . Moreover, the abundance change of PK was confirmed by slight increase in lba0957 gene expression observed by RT‐PCR (Supporting Information Fig.…”

Section: Discussionmentioning

confidence: 83%

Comparative proteomics of oxidative stress response of Lactobacillus acidophilus NCFM reveals effects on DNA repair and cysteine de novo synthesis

et al. 2017

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Probiotic cultures encounter oxidative conditions during manufacturing, yet protein abundance changes induced by such stress have not been characterized for some of the most common probiotics and starters. This comparative proteomics investigation focuses on the response by Lactobacillus acidophilus NCFM to H O simulating an oxidative environment. Bacterial growth was monitored by BioScreen and batch cultures were harvested at exponential phase for protein profiling of stress responses by 2D gel based comparative proteomics. Proteins identified in 19 of 21 spots changing in abundance due to H O were typically related to carbohydrate and energy metabolism, cysteine biosynthesis, and stress. In particular, increased cysteine synthase activity may accumulate a cysteine pool relevant for protein stability, enzyme catalysis, and the disulfide-reducing pathway. The stress response further included elevated abundance of biomolecules reducing damage such as enzymes from DNA repair pathways and metabolic enzymes with active site cysteine residues. By contrast, a protein-refolding chaperone showed reduced abundance, possibly reflecting severe oxidative protein destruction that was not overcome by refolding. The proteome analysis provides novel insight into resistance mechanisms in lactic acid bacteria against reactive oxygen species and constitutes a valuable starting point for improving industrial processes, food design, or strain engineering preserving microorganism viability.

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“…Amino acids are not only building-blocks in protein synthesis but some are precursors and carbon sources for the TCA cycle (Fig. 43 In comparison with the metabolites detected in the control group, choline and O-phosphocholine were uniquely detected in the Cd 2+ -treated group. The consistency between the down-regulation noted in amino acids and proteins including EF-Tu, 50S ribosomal protein L7/L12, trigger factor, peroxiredoxin and superoxide dismutase conrmed the disturbance in protein synthesis.…”

Section: Effects Of CD 2+ On the Proteome Of B Licheniformis Amentioning

confidence: 97%

Multi-omics based changes in response to cadmium toxicity in Bacillus licheniformis A

et al. 2015

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Cadmium (Cd), a widespread substance with high toxicity and persistence, is known to cause a broad range of adverse effects in all living organisms. However, many microorganisms have special detoxification mechanisms for Cd. In this study, we investigated the response to Cd in Bacillus licheniformis A at the omics level. Proteomic biomarkers were identified, including peroxiredoxin, nucleoside diphosphate kinase, trigger factor, and elongation factor Tu. These proteins suggested that Cd could induce oxidative stress, disturbance in energy metabolism, and protein synthesis in B. licheniformis A. Furthermore, metabolites (glutamate, glycine, and S-adenosylhomocysteine) involved in the glutathione synthesis pathway were also affected. In addition, some metabolic biomarkers were validated by relating to respective proteins, including 50S ribosomal protein L7/L12, trigger factor, peroxiredoxin and superoxide dismutase in B. licheniformis A. This study provides new insights into the utilization of an omics approach in bacteria to assess the effects of Cd pollution in the environment.

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Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate

Cited by 47 publications

References 25 publications

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

Comparative proteomics of oxidative stress response of Lactobacillus acidophilus NCFM reveals effects on DNA repair and cysteine de novo synthesis

Multi-omics based changes in response to cadmium toxicity in Bacillus licheniformis A

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