Hydrogen producer isolated from agricultural wastewater and molasses

Glinwong, Chompunuch; Lertsriwong, Supattra; Chulalaksananukul, Warawut

doi:10.1016/j.egypro.2017.10.081

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…However, it is important to note that the quality of molasses depends on the source and maturity of the sugar cane, the count of sugar extracted, the extraction process, and how the extract fermentation does. [10] reported that molasses in the growth medium increased the production of secondary metabolites and pigments. According to [11], bacterial…”

Section: Introductionmentioning

confidence: 99%

Utilization of molasses wastewater for medium production of Bacillus cereus

Feliatra

Batubara

Taufan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study aims to analyze the potential of molasses wastewater as a production medium for the Bacillus cereus SN7 and B. cereus consortium. The research was conducted experimentally using several B. cereus strains such as B. cereus SN7, B. cereus SP4, B. cereus S5, B. cereus Xmb051, and B. cereus BF2. The Analysis of the potential of molasses wastewater as a production medium was carried out by growing the B. cereus SN7 and B. cereus consortium on molasses liquid waste medium with added liquid skim milk. In the comparison, the nutrient broth medium was used as a positive control, and sterile distilled water as a negative control. Bacterial growth measurements were carried out for 24 hours with different observations, sequentially, 0, 6, 12,18, and 24 hours and observed every six hours using the total plate count method. It can be shown that the highest cell biomass is 1,029 g/ml and the optimum growth time of each treatment is at 18th hours. Thus, the use of molasses as a bacterium medium can be used for the production of B. cereus SN7, and B. cereus consortium.

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Section: Introductionmentioning

confidence: 99%

Utilization of molasses wastewater for medium production of Bacillus cereus

Feliatra

Batubara

Taufan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Construction of novel technologies leading to secure, sustainable, economically reasonable H 2 generation is in vital demand. Anaerobic bacteria such as Clostridium , Enterobacter , Thermotoga , and Bacillus can produce hydrogen through dark fermentation. Other fermented liquid bioenergy products are acetone, ethanol, and butanol produced from renewable agro‐industrial wastes by anaerobic bacteria .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of biohydrogen production from sustainable orange peel wastes usingEnterobacterspecies isolated from domestic wastewater

et al. 2018

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Summary Five facultative anaerobic bacterial isolates were recovered from domestic wastewater. These isolates were identified based on the 16S rRNA as Enterobacter aerogenes (one isolate), Enterobacter cloacae (two isolates), and Cronobacter sakazakii (three isolates). These isolates were examined for their potential to evolve hydrogen on a glucose medium. The most potent hydrogen‐producing isolates, E aerogenes (KY549389) and E cloacae (KY524293), were examined for their capacity to generate hydrogen, acetone, butanol, and ethanol using orange peel (OP) hydrolysate. OP powder was pretreated with n‐hexane to remove the toxicity of d‐limonene. Different concentrations (4%, 6%, and 8% w/v) of limonene‐free OP were subjected to the boiling water (temperature of 100°C) or acid (HCl) treatments. The maximum fermentative H2 production of 1700 and 1620 mL/L was obtained from 6% OP hydrolysate extracted with boiling water using facultative anaerobic E aerogenes (KY549389) and E cloacae (KY524293), respectively. Hydrogen production efficiency was 0.99 and 1.19 mol H2/mol glucose for E aerogenes and E cloacae, respectively. The total fermentative acetone, butanol, and ethanol (ABE) generated by E aerogenes and E cloacae were 0.78 and 0.38 g/L including acetone (0.05 and 0.04 g/L), butanol (0.011 and 0.013 g/L), and ethanol (0.71 and 0.32 g/L), respectively. The maximum ABE productivity was 0.01 and 0.005 g/L/h generated at 60 g/L OP hydrolysate by E aerogenes and E cloacae, respectively. These strains were positive for nitrogen fixation (nitrogenase) capability estimated by the acetylene reduction assay. Application of OP hydrolysate without the addition of any nutritional components or reducing agent is considered an eco‐friendly, economical, and commercial substrate for desired biofuel production.

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