Hydrogen production and transcriptional analysis of nifD, nifK and hupS genes in Rhodobacter sphaeroides O.U.001 grown in media with different concentrations of molybdenum and iron

Kars, Gökhan; Gündüz, Ufuk; Yücel, Meral; Türker, Lemi; Eroğlu, İnci

doi:10.1016/j.ijhydene.2006.06.021

Cited by 76 publications

(22 citation statements)

References 24 publications

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“…On the other hand, addition of vitamins and trace elements did not enhance the hydrogen production at all; hydrogen productions were within the same range. This result is supported by the findings of Kars et al [10] who utilized R. sphaeroides O.U.001 and reported that there was a growth delay and almost no hydrogen production in the defined medium which had no iron, showing that iron is vital for cellular functions and the hydrogen production. They suggested 0.1 mM of ferric citrate as optimum concentration for hydrogen production.…”

Section: Resultssupporting

confidence: 81%

Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent

Uyar

Schumacher

Gebicki

et al. 2008

Bioprocess Biosyst Eng

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Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydrogen yield increased from 0.3 to 1.0 L/L(culture) by addition of iron to the effluent solution.

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Section: Resultssupporting

confidence: 81%

Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent

Uyar

Schumacher

Gebicki

et al. 2008

Bioprocess Biosyst Eng

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“…On the contrary, both sulfate and iron additions induced the reestablishment of H 2 production, but did not have any significant effect on cell growth. Actually, a decrease in H 2 production upon sulfur deprivation of PNSB cultures was previously reported [31], as well as the effect of low Fe concentrations on the expression levels of nifD, one of the nitrogenase genes [32]. As nitrogenase is an enzyme containing FeeS clusters [33]…”

Section: H 2 Production Using the Vegetable Waste Derived (Vwd) Mediummentioning

confidence: 85%

A Rhodopseudomonas palustris nifA* mutant produces H2 from -containing vegetable wastes

Adessi

McKinlay

Harwood

et al. 2012

International Journal of Hydrogen Energy

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“…It was shown that nitrogenase gene expression decreases in Rhodobacter sphaeroides when the cells starved iron and molybdenum [16]. The DFE used in this study contained enough molybdenum, but iron concentration was very low (Table 1); therefore, 0.1 mM of Fe-citrate was added into the DFE as a supplement in order to enhance the hydrogen production.…”

Section: Experimental Section Bacteria and Culture Mediamentioning

confidence: 98%

Hydrogen production by hup− mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors

Uyar

Gürgan

Özgür

et al. 2015

Bioprocess Biosyst Eng

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Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenase-deficient (hup (-)) mutant strains of Rhodobacter capsulatus by means of hydrogen production and biomass growth. The tests were conducted in small-scale (50 mL) batch and large-scale (4 L) continuous photobioreactors in indoor conditions under continuous illumination. In small scale batch conditions, maximum cell concentrations were 0.92 gdcw/L c and 1.50 gdcw/L c, hydrogen yields were 34 % and 31 %, hydrogen productivities were 0.49 mmol/(L c·h) and 0.26 mmol/(Lc·h), for hup (-) and wild-type cells, respectively. In large-scale continuous conditions, maximum cell concentrations were 1.44 gdcw/L c and 1.87 gdcw/L c, hydrogen yields were 48 and 46 %, and hydrogen productivities were 1.01 mmol/(L c·h) and 1.05 mmol/(L c·h), for hup (-) and wild-type cells, respectively. Our results showed that Rhodobacter capsulatus hup (-) cells reached to a lower maximum cell concentration but their hydrogen yield and productivity were in the same range or superior compared to the wild-type cells in both batch and continuous operating modes. The maximum biomass concentration, yield and productivity of hydrogen were higher in continuous mode compared to the batch mode with both bacterial strains.

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Hydrogen production and transcriptional analysis of nifD, nifK and hupS genes in Rhodobacter sphaeroides O.U.001 grown in media with different concentrations of molybdenum and iron

Cited by 76 publications

References 24 publications

Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent

Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent

A Rhodopseudomonas palustris nifA* mutant produces H2 from -containing vegetable wastes

Hydrogen production by hup− mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors

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