Hydrogen Production by Clostridium cellulolyticum a Cellulolytic and Hydrogen-Producing Bacteria Using Sugarcane Bagasse

Abstract

Hydrogen (H 2) production by Clostridium cellulolyticum was investigated. Anaerobic batch reactors were operated with cellobiose (2 g/L) and pretreated sugarcane bagasse (SCB) (2 g/L) using a hydrothermal system to observe the effects of carbon source on H 2 production. Salts (NH 4 Cl, NaCl, MgCl 2 and CaCl 2) and vitamins (biotin, nicotinamide, p-aminobenzoic acid, thiamine, pantothenic acid, pyridoxamine, cyanocobalamin, riboflavin, folic and lipoic acid) were supplemented from stock solutions at different volumes percentages, ranging from 0 to 5%. The optimal concentration was 2.5% and the strain used both substrates and produced H 2 which was higher for cellobiose (14.9 ± 0.2 mmol/L) than for SCB (7.6 ± 0.2 mmol/L), although the λ phase was much smaller when SCB (59.9 h) was used in relation to the assay with cellobiose (164 h). H 2 was produced from SCB primarily through the fermentation of lactic and acetic acids.

Iron/carbon conductive materials for enhancing anaerobic treatment of synthetic acrylamide wastewater

“…7(a,b)). Previous studies have pointed out that the Gibbs free energy of propionic acid metabolism is positive at pH 7, and the degradation of propionic acid is difficult 38‐40 . Therefore, in the batch experiments of this study, a high concentration of propionic acid was detected in the subsequent detection (Fig.…”

Produção de hidrogênio e ácidos orgânicos a partir do bagaço de cana-de-açúcar e licor de pentoses em reatores em batelada com consórcio de microorganismos autóctones

“…O efluente foi uma solução de H 2 SO 4 0,005M, a um fluxo de 0,5 mL.min-1, temperatura do forno de 43ºC e injeção de 100 μL. O limite de detecção dos ácidos variam de 10-1.000 mg.L -1 (AHMAD et al, 2021) O gás hidrogênio foi determinado por cromatografia gasosa por meio da retirada de 500 µL de amostra gasosa do headspace utilizando-se seringa gastight Em todos os experimentos realizados nesse estudo, a concentração de H 2 foi calculada considerando o volume injetado, a área cromatográfica (promovida pela calibração e em mmol) e o volume do headspace, conforme Braga et al (2019). Após as medições de hidrogênio, foi capaz de eleger o melhor tipo de bagaço para os ensaios fermentativos com base na maior produção de H 2 durante o período observado.…”