Hydrogen production by dark fermentation using a new low-cost culture medium composed of corn steep liquor and cassava processing water: Process optimization and scale-up

Martínez-Burgos, Walter José; Sydney, Eduardo Bittencourt; Paula, Dieggo Rodrigues de; Medeiros, Adriane Bianchi Pedroni; Carvalho, Júlio César de; Molina, Denisse; Soccol, Carlos Ricardo

doi:10.1016/j.biortech.2020.124370

Cited by 46 publications

(12 citation statements)

References 35 publications

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“…At a dilution rate of D 1 = 0.4 day −1 and a concentration of the effluent of 25 g/L (for BR 1 ), respectively, a dilution rate of D 2 = 0.05 day −1 for BR 2 for the 30th day of the experiment, the following results are obtained: for BR 1 , the daily yield of biohydrogen is 0.319 dm 3 of biogas from a 1 L working volume at a concentration of hydrogen in biogas of 5.35% volumetric, pH = 5.35; for BR 2 , the daily yield of biomethane is 0.392 dm 3 of biogas from a 1 L working volume at a methane concentration in biogas of 68% volumetric, pH = 7.4. Biohydrogen yields are close to those presented in [13], where biohydrogen is obtained from a mixture of CSL and a type of grass (cassava). Biomethane yields are higher than those reported in [25], where biomethane is derived from poorer organic urban wastewater.…”

Section: Discussionsupporting

confidence: 82%

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Two-Phase Anaerobic Digestion of Corn Steep Liquor in Pilot Scale Biogas Plant with Automatic Control System with Simultaneous Hydrogen and Methane Production

et al. 2022

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Experimental studies of two-phase anaerobic digestion of corn steep liquor in semi-continuous automatic and semi-automatic modes of operation of a cascade of two anaerobic bioreactors with monitoring and control systems were performed. Corn steep liquor—a waste product from the process of treating corn grain for starch extraction—was used as a substrate in the process of anaerobic digestion with simultaneous hydrogen and methane production. The daily yields of biohydrogen in bioreactor 1 of the cascade (with a working volume of 8 dm3) are variable. In good operation, they are in the range of 0.7 to 1.0 L of biogas from a 1 dm3 working volume of the bioreactor, and the optimal pH is in the range of 5.0–5.5. The concentration of hydrogen in the biogas from the hydrogen bioreactor 1 is in the range of 14–34.7%. The daily yields of biomethane in bioreactor 2 of the cascade (with a working volume of 80 dm3) vary in the range 0.4 to 0.85 l of biogas from a 1 dm3 working volume of the bioreactor, and the concentration of methane in the biogas from bioreactor 2 is high and remains practically constant (in the range 65–69%). At a dilution rate of 0.4 day−1 and an organic loading rate of 20 gL for bioreactor 1, respectively, and a dilution rate of 0.05 day−1 for bioreactor 2, the best results were obtained. The computer control system is presented. Some energetical considerations were discussed.

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Section: Discussionsupporting

confidence: 82%

“…In the case of TPAD, the waste obtained can also be used as natural fertilizer in agriculture. The corn steep liquor (CSL) selected as the raw material was used (alone or in mixtures) to produce biohydrogen or biomethane [13,14]. Corn steep liquor is a cheap alternative to yeast extract for numerous biotechnological industrial processes.…”

Section: Introductionmentioning

confidence: 99%

Two-Phase Anaerobic Digestion of Corn Steep Liquor in Pilot Scale Biogas Plant with Automatic Control System with Simultaneous Hydrogen and Methane Production

et al. 2022

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“…1), the isolate strain was identi ed as Clostridium butyricum with 99% similarity. The phylogenetic tree constructed with the neighbour-joining method revealed that the C. butyricum was included in the same subcluster with C. demonstrate that the concentration of CSL, whey, and the fermentation time affect hydrogen production via dark fermentation [22,27]. The inoculum rate was not signi cant (p ≤ 0.05), which may have been because large inoculum volumes were tested; an inoculum rate of 10% was then used for all experiments.…”

Section: Clostridium Butyricum Identi Cationmentioning

confidence: 99%

Biohydrogen Production from Agro-industrial Wastes Using Clostridium beijerinckii and Isolated Bacteria as Inoculum

et al. 2021

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The search for renewable and sustainable sources of energy has been one of the main goals of society in recent years, especially to reduce the environmental impacts of fossil fuels. One promising alternative is the production of hydrogen, which does not emit greenhouse gases and can be produced from agroindustrial wastes. The Clostridium genus is recorded as having high hydrogen yields compared to other genus, with several producing species. The objective of this work was to evaluate biohydrogen production potential of four agro-industrial residues, which were soft drink wastewater, corn steep liquor, cheese whey, and expired Guaraná soft drink, using one model strain Clostridium beijerinckii ATCC 8260 and newly isolated Clostridium butyricum DEBB-B348. The agro-industrial wastes were characterised in terms of monosaccharide, organic acid, amino acid, cation, and anion concentrations and compared to the literature. After performing subsequent experimental designs, the signi cant factors were cheese whey concentration, corn steep liquor concentration, and fermentation time for C. beijerinckii, and corn steep liquor concentration and fermentation time for C. butyricum (p ≤ 0.05), with an R 2 of 0.950 and 0.895, respectively. The maximum hydrogen volume production was 18.5 ± 1.68 mL and 27.4 ± 1.84 mL for each strain, respectively. The C. butyricum 16s rRNA gene phylogenetic tree and the carbohydrate, organic acid, and amino acid kinetics of the optimum medium are also presented. These results indicate a potential hydrogen production process utilising less expensive substrates, proposing more proper disposal for agro-industrial wastes and using an isolated strain with high yield.

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“…For example, the cost of potassium carbonate used for wet conversion of PG by potash solution can reach 1,000-1100 USD/t (Yelatontse and Mukhachev, 2021). However, the cost of using agricultural organic waste (peanut bran, straw, and soybean) for microbial fluid by biological fermentation is only 100-300 USD/t, and that of the agro-industrial effluent medium is even as low as 75-83 USD/t (Martinez-Burgos et al, 2021). The biological flotation/purification technology of high EPS-producing bacteria (red yeast) is simpler and faster.…”

Section: Biomechanism Analysis Of Rho For Safe Utilization Of Pg and Tgmentioning

confidence: 99%

Red Yeast Improves the Potential Safe Utilization of Solid Waste (Phosphogypsum and Titanogypsum) Through Bioleaching

Chen

Yu-qi

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Phosphogypsum (PG) and titanium gypsum (TG), as a by-product (solid waste) in phosphate fertilizer and titanium dioxide industry, are causing serious environmental hazards. The resource/harmless application of PG and TG is the development trend in the future. The biological function of red yeast (Rho: Rhodotorula mucilaginosa) can effectively reduce the concentration of pollutants in the environment and has the potential of biological flotation/purification of mineral solid waste. In this study, the bioremediation mechanism and safe utilization efficiency of Rho for different contents of PG and TG were explored by using its biological flotation function. The X-ray fluorescence spectrometry (XRF) results showed that F was the main toxic element in PG and TG, and Pb and Cd did not reach the detection limit. The processing capacity of Rho for PG (>10 g/ml) is higher than that of TG (<5 g/ml). After bioleaching by Rho, the proportion of F in PG and TG solid decreased by 61.45–63.79% and 49.45–59.19%, respectively. The results of three-dimensional fluorescence, extracellular polymeric substance (EPS) extraction, X-ray diffraction (XRD), and scanning electron microscopy (SEM) confirmed that Rho could accelerate the release of harmful elements (F) in PG and TG. SEM showed that Rho cells and secretions adhered and wrapped on PG/TG, causing PG/TG decomposition and fragmentation. In addition, the adsorption of EPS and the formation of Ca5(PO4)3F are two main ways for Rho to remove F. Furthermore, under the condition of high concentration bioleaching, Rho can accelerate the release and utilization of P in PG, which is not only for the re-precipitation of Ca5(PO4)3F but also conducive to the reproduction and utilization of microorganisms. Meanwhile, the purification/safe reuse of PG by Rho is easier than that of TG. Therefore, the toxicity of PG and TG bioleaching by Rho can be greatly reduced, suggesting the huge potential of Rho in soil improvement and remediation.

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Hydrogen production by dark fermentation using a new low-cost culture medium composed of corn steep liquor and cassava processing water: Process optimization and scale-up

Cited by 46 publications

References 35 publications

Two-Phase Anaerobic Digestion of Corn Steep Liquor in Pilot Scale Biogas Plant with Automatic Control System with Simultaneous Hydrogen and Methane Production

Two-Phase Anaerobic Digestion of Corn Steep Liquor in Pilot Scale Biogas Plant with Automatic Control System with Simultaneous Hydrogen and Methane Production

Biohydrogen Production from Agro-industrial Wastes Using Clostridium beijerinckii and Isolated Bacteria as Inoculum

Red Yeast Improves the Potential Safe Utilization of Solid Waste (Phosphogypsum and Titanogypsum) Through Bioleaching

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