Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17

Liu, Yan; Peng, Yu; Song, Xin; Qu, Yinbo

doi:10.1016/j.ijhydene.2008.04.004

Cited by 186 publications

(92 citation statements)

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“…These results indicate that strain G3 exhibits high hydrolysis activity on Avicel under mesophilic conditions and that oligosaccharide production is even higher than that reported for monocultures of thermophilic Clostridium spp. (6,14,23,35).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Wang

Gao

Ren

et al. 2011

Appl Environ Microbiol

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A novel Shigella strain (Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40°C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel ؊1 and 6.25 mg g Avicel ؊1 h ؊1 , respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.Lignocellulosic biomass is abundant in nature, as well as in agricultural, forestry, and municipal wastes, and can be used as an excellent bioconversion feedstock. Biomass-derived saccharides, such as glucose, cellobiose, and other minor sugars, can be readily fermented by appropriate microbes into bioenergy products, such as hydrogen, ethanol, biodiesel, and other commodity chemicals. However, the high cost of converting biomass to sugars is the primary factor impeding establishment of a cellulosic-biofuels industry (24, 40). Lignocellulosic biofuels can be competitive on an industrial scale if efficient technologies can be developed (29,39). Currently, the most efficient process for utilization of cellulose as a feed stock is either a three-step process (separate hydrolysis and fermentation [SHF]) involving separate pretreatment, cellulose hydrolysis (i.e., saccharification), and hexose and pentose fermentation steps or a two-step process (simultaneous saccharification and fermentation [SSF]) involving separate pretreatment and simultaneous saccharification of hexose and pentose fermentation (48, 64). Combining hydrolysis of cellulose with simultaneous fermentation of hexose and pentose in a single process, i.e., direct microbial conversion (DMC), is an ideal strategy for converting cellulosic biomass to ethanol. However, no single microorganism/community can implement DMC with high efficiency (67). In any of these configurations, rapid and efficient saccharification is critical for developing competitive biotechnologies for cellulosic-biofuel production.In nature, cellulose is hydrolyzed to oligosaccharides by microorganisms, mainly fungi (e.g., brown-, white-, and soft-rot fungi) and bacteria (e.g., Clostridium and Cellulomonas), which produce either free cellulolytic enzymes or extracellular enzyme complexes known as cellulosomes (12). Many white-rot Basidiomycetes and some Actinomycetes have been employed for hydrolyzing lignocellulosic materials. For example, Trichoderma reesei has shown the highest cellulolytic activity currently known (27)....

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Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Wang

Gao

Ren

et al. 2011

Appl Environ Microbiol

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“…Liu ect. got the maximum ratio butyric acid production rate of 0.13 g/(g-DCW⋅h) by similar system with C. thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17 [29]. In contrast, the maximum ratio butyric acid production rate of 0.18 g/(g-DCW⋅h) obtained by co-culturing of (X3+C4+B20) has a significant advantage in the fermentation of lignocellulosic biomass in this study.…”

Section: Butyric Acid Fermentation By Mixed Strainsmentioning

confidence: 61%

Novel Butyric Acid Fermentation Strains and Their Performance in Fermenting Rice Straw by Pure and Mixed Culturing

Zhang¹,

Sun²,

Zhang³

et al. 2017

dteees

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Abstract. Synergistic metabolism among mixed culture can be utilized to hydrolise and ferment cellulose and hemicellulose in rice straw to avoid high cost of saccharification and its complex procedure. Three buryric acid producing strains cellulose-decomposing bacteria named Clostridium cadaveris C4, cellubiose fermenting bacteria named C. butyricum B20, xylan-degrading bacteria named C. butyricum X3 respectively were isolated from undefined mixed culture which was enriched and cultured from rice straw. The fermentation experiment of rice straw was carried out to increase the burytic acid conversion rate with three single strain and their mixed strains. Fermentation results showed that co-fermentation with two or three strains can significantly improve the degradation of rice straw and yield of butyric acid because of synergistic effect in or among different metabolic strains. Among the experiment, co-culture of three strains with inoculum of 1.1 g-DCW/L got the butyric acid production rate 0.18 g/(g-DCW⋅h) during logarithmic period which increased by 50%, 200% and 63.64% compared with C4, B20 and X3 respectively.The results of the study provide the methodological guidance and bacterial strain resources for the butyric acid biotransformation from lignocellulosic biomass.

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“…Fermentation of hydrolysates from Miscanthus hydrolysates by Caldicellulosiruptor saccharolyticus and Thermotoga elfi, pretreated by alkali, resulted in 3.4 and 3.2 mol H 2 mol glucose -1 equivalent, respectively (de Vrije et al, 2009). Corn stover and corn stover cornstalk have been investigated for H 2 production capacity by many Datar et al, 2007;Liu et al, 2008b;Liu & Cheng, 2010;Ren et al, 2010). Pure culture studies on Thermoanaerobacterium thermosaccharolyticum on corn stover hydrolysates showed maximum of 2.7 mol H 2 mol glucose -1 equivalent diluted corn stover hydrolysates that contained a mixture of glucose, xylose and arabinose (total sugar concentration, 10 g L -1 ) .…”

Section: Wwwintechopencommentioning

confidence: 99%

“…A coculture of Clostridium thermocellum and Thermoanaerobacterium thermosaccharolyticum grown on hydrolysate made from 5 g L -1 of corn stalk and corn cob powder (no pretreatment), resulted in 1.80 mol H 2 mol glucose -1 (Liu et al, 2008b). Clostridium AK14 was used to degrade hemp (both stem and leaf), grass, paper and straw (Almarsdottir et al, 2010).…”

Section: Wwwintechopencommentioning

confidence: 99%

“…Clostridium AK14 was used to degrade hemp (both stem and leaf), grass, paper and straw (Almarsdottir et al, 2010). Highest yields were observed on grass pretreated with 0.75% sulfuric acid and (Almarsdottir et al, 2010;Geng et al, 2010;Levin et al, 2006;Liu et al, 2003;Liu et al, 2008b;Nguyen et al, 2008). Various sources of cellulose have been used, e.g.…”

Section: Wwwintechopencommentioning

confidence: 99%

See 1 more Smart Citation

Ethanol and Hydrogen Production with Thermophilic Bactera from Sugars and Complex Biomass

Sveinsdóttir¹,

Sigurbjörnsdóttir²,

Örlygsson³

2011

Progress in Biomass and Bioenergy Production

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Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17

Cited by 186 publications

References 31 publications

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Novel Butyric Acid Fermentation Strains and Their Performance in Fermenting Rice Straw by Pure and Mixed Culturing

Ethanol and Hydrogen Production with Thermophilic Bactera from Sugars and Complex Biomass

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