Hydrogen regulates the M1/M2 polarization of alveolar macrophages in a rat model of chronic obstructive pulmonary disease

Su, Jing-Chao; Zhang, Yi; Cheng, Chen; Zhu, Yinan; Ye, Yu-Meng; Sun, Yongkang; Xiang, Shui-Ying; Wang, Yuan; Liu, Zi-Bing; Zhang, Xinfang

doi:10.1080/01902148.2021.1919788

Cited by 10 publications

(4 citation statements)

References 51 publications

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“…Although powerful techniques are available for the study of the arginase pathway and its involvement in immunology and physiology, the determination of arginase activity is important. Other techniques, such as real-time PCR, quantify only the gene count, and still others, such as ELISA, only assess the concentration of the arginase protein [ 6 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of the arginase activity assay micromethod for macrophages and sera

Nzoumbou-Boko,

Zolipou,

Yambiyo

et al. 2023

BMC Res Notes

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Objective We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke’s method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice, and in purified bovine liver arginase was compared using the conventional and optimized micromethods. Results The sensitivity of both micromethods was comparable. However, our optimized method has the following advantages: it uses small sample volumes (6 µl per assay vs. 50 µl) and reagent volumes (200 µl vs. 400 µl), it can be carried out in a single microplate well, thereby minimizing handling, and it requires fewer materials and utilizes readily available equipment. Our optimized method proved to be applicable and well suited for small-volume samples and resource-poor laboratories.

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Section: Discussionmentioning

confidence: 99%

Optimization of the arginase activity assay micromethod for macrophages and sera

Nzoumbou-Boko,

Zolipou,

Yambiyo

et al. 2023

BMC Res Notes

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“…To date, some research on airway inf lammation, M1/M2 macrophage polarization and secretion of TNF-α have been reported in asthmatic and COPD animal models and in vitro (37)(38)(39). Some in vitro M1/M2 research is also performed in human primary monocyte-derived macrophages (40) and RAW264.7 cells (35,41).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of budesonide on LPS‑induced podocyte injury by modulating macrophage M1/M2 polarization: Evidence from in vitro and in silico studies

et al. 2022

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Budesonide (Bud), one of the most widely used lung medicines, is currently used as a repurposing medicine for immunoglobulin A nephropathy (IgAN) treatment. The progression of IgAN is related to inflammation involving macrophages and podocytes. The present study aimed to explore the effects of Bud on classically activated (M1)/alternatively activated (M2) macrophage polarization and podocyte injury under lipopolysaccharide (LPS)-induced inflammatory stress in vitro. Anti-inflammatory bioinformation of Bud was identified based on the Gene Expression Omnibus database. RAW264.7 cells were treated with normal medium, LPS, curcumin (Cur, positive control), or Bud 5, 10, or 20 µM. The expression levels of inducible nitric oxide synthase (iNOS), TNF-α, mannose receptor (CD206) and arginase (Arg)-1 were quantified by western blotting. The collected supernatants from macrophages were termed (Nor)MS, (LPS)MS, (Cur)MS and (Bud)MS. The TNF-α, IL-1β and nitric oxide (NO) levels in the supernatants were evaluated by ELISA and Griess assay. The podocytes were cultured in different supernatants and their survival rates were assessed by bromodeoxyuridine assay. TNF signaling is an important pathway by which Bud exerts anti-inflammatory activities. Compared with the LPS group, 5, 10 and 20 µM Bud significantly increased Arg-1 and decreased iNOS expression (Six: P<0.05) and 20 µM Bud significantly increased Arg-1 and CD206 and decreased iNOS and TNF-α expression (Four: P<0.05). Cur significantly decreased iNOS and TNF-α expression (Two: P<0.05). Compared with LPS, 5, 10 and 20 µM Bud and Cur significantly decreased TNF-α, IL-1β and NO levels (All: P<0.05). The podocyte survival rates of (Bud)MS and (Cur)MS were significantly higher than those of (LPS)MS (Four: P<0.05). The protective effect of Bud on podocyte injury is related to its modulation of M1/M2 polarization.

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“…In addition, salidroside inhibited the percentage and protein expression of iNOS + M1 macrophages in AMs by the inhibition of JNK/c-Jun. Moreover, hydrogen intervention reversed the increased levels of IL6, TNFα, and TGFβ while decreasing the levels of IL10 with an improvement in the lung function in CS exposure with LPS models [ 183 ]. Sun et al observed that ergosterol treatment reduced the levels of M1-associated cytokines (IL6 and TNFα) while increasing M2-associated cytokines (IL10 and TGFβ) in BALF.…”

Section: Macrophage-targeting Copd Treatmentmentioning

confidence: 99%

Macrophage Polarization and Functions in Pathogenesis of Chronic Obstructive Pulmonary Disease

Kim,

Lim,

Shin

2024

IJMS

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Chronic obstructive pulmonary disease (COPD), the major leading cause of mortality worldwide, is a progressive and irreversible respiratory condition characterized by peripheral airway and lung parenchymal inflammation, accompanied by fibrosis, emphysema, and airflow limitation, and has multiple etiologies, including genetic variance, air pollution, and repetitive exposure to harmful substances. However, the precise mechanisms underlying the pathogenesis of COPD have not been identified. Recent multiomics-based evidence suggests that the plasticity of alveolar macrophages contributes to the onset and progression of COPD through the coordinated modulation of numerous transcription factors. Therefore, this review focuses on understanding the mechanisms and functions of macrophage polarization that regulate lung homeostasis in COPD. These findings may provide a better insight into the distinct role of macrophages in COPD pathogenesis and perspective for developing novel therapeutic strategies targeting macrophage polarization.

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Hydrogen regulates the M1/M2 polarization of alveolar macrophages in a rat model of chronic obstructive pulmonary disease

Cited by 10 publications

References 51 publications

Optimization of the arginase activity assay micromethod for macrophages and sera

Optimization of the arginase activity assay micromethod for macrophages and sera

Protective effects of budesonide on LPS‑induced podocyte injury by modulating macrophage M1/M2 polarization: Evidence from in vitro and in silico studies

Macrophage Polarization and Functions in Pathogenesis of Chronic Obstructive Pulmonary Disease

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