Hydrogen-Rich Saline Improves Survival and Neurological Outcome After Cardiac Arrest and Cardiopulmonary Resuscitation in Rats

Huo, Tingting; Zeng, Yi; Liu, Xiaonan; Sun, Li; Han, Huan-zhi; Chen, Hongguang; Lu, Zhihong; Huang, Yi; Nie, Huang; Dong, Hailong; Xie, Keliang; Xiong, Lize

doi:10.1213/ane.0000000000000303

Cited by 37 publications

(30 citation statements)

References 32 publications

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“…Moreover, in a CA/CPR swine model, increased pro-inflammatory cytokines expression, such as IL-1β and TNFα, were shown after resuscitation and played an important role in post-cardiac arrest syndrome [ 33 ]. Importantly, hydrogen-rich saline treatment could reduce the production of inflammatory cytokines, such as TNFα and IL-1β, after CA/CPR procedure so that it could improve survival condition and neurological outcome of rats [ 34 ]. Therefore, these studies indicated that reducing inflammation level might be a potential therapy to improve clinical outcome.…”

Section: Discussionmentioning

confidence: 99%

RNase alleviates neurological dysfunction in mice undergoing cardiac arrest and cardiopulmonary resuscitation

Chan

Zhang

et al. 2017

Oncotarget

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Cardiac arrest (CA) is one of the leading lethal factors. Despite cardiopulmonary resuscitation (CPR) procedure has been consecutively improved and lots of new strategies have been developed, neurological outcome of the patients experienced CPR is still disappointing. Ribonuclease (RNase) has been demonstrated to have neuroprotective effects in acute stroke and postoperative cognitive impairment, possibly through acting against endogenous RNA that released from damaged tissue. However, the role of RNase in post-cardiac arrest cerebral injury is unknown. In the present study, we investigated the role of RNase in neurological outcome of mice undergoing 5 minutes of CA and followed by CPR. RNase or the same dosage of normal saline was administrated. We found that RNase administration could: 1) improve neurologic score on day 1 and day 3 after CA/CPR performance; 2) improve memory and learning ability on day 3 after training in contextual fear-conditioning test; 3) reduce extracellular RNA (exRNA) level in plasma and hippocampus tissue, and hippocampal cytokines mRNA production on day 3 after CA/CPR procedure; 4) attenuate autophagy levels in hippocampus tissue on day 3 after CA/CPR procedure. In conclusion, RNase could improve neurological function by reducing inflammation response and autophagy in mice undergoing CA/CPR.

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Section: Discussionmentioning

confidence: 99%

RNase alleviates neurological dysfunction in mice undergoing cardiac arrest and cardiopulmonary resuscitation

Chan

Zhang

et al. 2017

Oncotarget

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“…A recent report emphasizes the critical role in SAH apoptosis due to the suppression of inflammatory responses through NF-κB and NLRP3 inflammasomes [300]. Additional benefits of hydrogen treatment have also been reported in animal models for other types of brain injury, such as those promoted by cardiac arrest and cardiopulmonary resuscitation [128], survival of retinal ganglion neurons after optic nerve crash [301] and neuroinflammation by sepsis [130].…”

Section: Hydrogen In Injuriesmentioning

confidence: 99%

“…Inhalation of hydrogen or using hydrogen-saline solutions has proven to be beneficial for brain damage produced by traumatic injury in rats [126] [127]. Hydrogen-rich saline solutions have been effective after rat brain ischemic damage produced by cardiac arrest or vascular causes [128] [129]. Finally, the protective effects of inhalation of hydrogen gas in mice have been also observed in damaged brains after inflammation [130].…”

Section: Hydrogen and Ischemia/reperfusion Injurymentioning

confidence: 99%

Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine

Nicolson¹,

Ferreira²,

Settineri³

et al. 2016

IJCM

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Here we review the literature on the effects of molecular hydrogen (H2) on normal human subjects and patients with a variety of diagnoses, such as metabolic, rheumatic, cardiovascular and neurodegenerative and other diseases, infections and physical and radiation damage as well as effects on aging and exercise. Although the effects of H2 have been studied in multiple animal models of human disease, such studies will not be reviewed in depth here. H2 can be administered as a gas, in saline implants or infusions, as topical solutions or baths or by drinking H2-enriched water. This latter method is the easiest and least costly method of administration. There are no safety issues with hydrogen; it has been used for years in gas mixtures for deep diving and in numerous clinical trials without adverse events, and there are no warnings in the literature of its toxicity or longterm exposure effects. Molecular hydrogen has proven useful and convenient as a novel antioxidant and modifier of gene expression in many conditions where oxidative stress and changes in gene expression result in cellular damage.

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“…Cardiac arrest was induced by asphyxia. 18 Animals were anesthetized with pentobarbital (45 mg/kg) and the trachea was orally intubated with a 14-gauge plastic catheter. After tracheal intubation, polyethylene catheters (Becton Dickinson, Franklin Lakes, NJ, USA) were placed through the left femoral artery and vein into the abdominal aorta and the inferior vena cava, respectively.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

Inhalation of high concentration hydrogen gas improves short-term outcomes in a rat model of asphyxia induced-cardiac arrest

et al. 2018

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Cardiogenic global brain hypoxia-ischemia is a devastating medical problem that is associated with unfavorable neurologic outcomes. Low dose hydrogen gas (up to 2.9%) has been shown to be neuroprotective in a variety of brain diseases. In the present study, we investigated the protective effect of water by electrolysis-derived high concentration hydrogen gas (60%) in a rat model of asphyxia induced-cardiac arrest and global brain hypoxia-ischemia. High concentration hydrogen gas was either administered starting 1 hour prior to cardiac arrest for 1 hour and starting 1 hour post-resuscitation for 1 hour (pre- & post-treatment) or starting 1 hour post-resuscitation for 2 hours (post-treatment). In animals subjected to 9 minutes of asphyxia, both therapeutic regimens tended to reduce the incidence of seizures and neurological deficits within 3 days post-resuscitation. In rats subjected to 11 minutes of asphyxia, significantly worse neurological deficits were observed compared to 9 minutes asphyxia, and pre- & post-treatment had a tendency to improve the success rate of resuscitation and to reduce the seizure incidence within 3 days post-resuscitation. Findings of this preclinical study suggest that water electrolysis-derived 60% hydrogen gas may improve short-term outcomes in cardiogenic global brain hypoxia-ischemia.

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Hydrogen-Rich Saline Improves Survival and Neurological Outcome After Cardiac Arrest and Cardiopulmonary Resuscitation in Rats

Abstract: Hydrogen-rich saline treatment improved survival and neurological outcome after cardiac arrest/resuscitation in rats, which was partially mediated by reducing oxidative stress, inflammation, and apoptosis.

Cited by 37 publications

References 32 publications

RNase alleviates neurological dysfunction in mice undergoing cardiac arrest and cardiopulmonary resuscitation

RNase alleviates neurological dysfunction in mice undergoing cardiac arrest and cardiopulmonary resuscitation

Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine

Inhalation of high concentration hydrogen gas improves short-term outcomes in a rat model of asphyxia induced-cardiac arrest

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