Hydrogen‐Rich Saline Protects against Ischemia/Reperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats

Abstract

Purpose. This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats. Methods. Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines. Results. Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants. Conclusion. Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

Molecular hydrogen: a preventive and therapeutic medical gas for various diseases

“…Xue, et al found that drinking hydrogen-rich electrolyzed water suppressed the dose-response effect of aspirin-induced gastric injury in a rat model [ 147 ]. HS injection also reduced the severity of acute pancreatitis [ 13 , 28 ] and I/R injury after pancreatic transplantation in rats [ 148 ].…”

MicroRNA files in the prevention of intestinal ischemia/reperfusion injury by hydrogen rich saline

“…From hydrogen gas to HRS, hydrogen as a potent and selective antioxidant has been applied in clinic and studied in various animal disease models, especially in the setting of I/R injury [23][24][25]. As a well-established key feature of I/R injury, oxidative stress mediated by highly toxic ROS like hydroxyl radicals and peroxynitrite has been a main therapeutic target in interventions for I/R injury.…”

Hydrogen in Transplantation: Potential Applications and Therapeutic Implications

“…Luo et al demonstrated that the intravenous administration of hydrogen-saturated saline to the recipient enhances the migration and proliferation capacity of bone marrow mesenchymal stem cells (BMSCs) to repair spinal cord injury by reducing the inflammatory response and oxidative stress in the injured area, suggesting that hydrogen and BMSC co-delivery is an effective method of improving BMSC transplantation in the treatment of spinal cord injury [12]. Intravenous hydrogen-rich saline administered via the tail vein at the beginning of reperfusion significantly diminished the severity of pancreatic IRI in rats, possibly by decreasing inflammation and oxidative stress [24].…”