Hydrogen-Rich University of Wisconsin Solution Attenuates Renal Cold Ischemia–Reperfusion Injury

Abe, Toyofumi; Li, Xiaokang; Yazawa, Koji; Hatayama, Naoyuki; Xie, Lin; Sato, Bunpei; Kakuta, Yoichi; Tsutahara, Koichi; Okumi, Masayoshi; Tsuda, Hidetoshi; Kaimori, Jun‐Ya; Isaka, Yoshitaka; Natori, Michiya; Takahara, Shiro; Nonomura, Norio

doi:10.1097/tp.0b013e318255f8be

Cited by 51 publications

(52 citation statements)

References 5 publications

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“…Therefore, the H 2 concentration, when mixing normal KHB and H 2 saturated KHB with 96 to 4 (vol/vol), is calculated as 29.3 mM theoretically. These results are consistent with previous reports showing the efficacy of hydrogen gas against cold I/R injury in other organs (11)(12)(13). Accordingly, we adopted the flow rate of 4%.…”

Section: Discussionsupporting

confidence: 91%

Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver

Shimada¹,

Wakayama²,

Fukai

et al. 2016

Artificial Organs

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Hydrogen gas reduces ischemia and reperfusion injury (IRI) in the liver and other organs. However, the precise mechanism remains elusive. We investigated whether hydrogen gas ameliorated hepatic I/R injury after cold preservation. Rat liver was subjected to 48-h cold storage in University of Wisconsin solution. The graft was reperfused with oxygenated buffer with or without hydrogen at 37° for 90 min on an isolated perfusion apparatus, comprising the H (+) and H (-) groups, respectively. In the control group (CT), grafts were reperfused immediately without preservation. Graft function, injury, and circulatory status were assessed throughout the perfusion. Tissue samples at the end of perfusion were collected to determine histopathology, oxidative stress, and apoptosis. In the H (-) group, IRI was indicated by a higher aspartate aminotransferase (AST), alanine aminotransferase (ALT) leakage, portal resistance, 8-hydroxy-2-deoxyguanosine-positive cell rate, apoptotic index, and endothelial endothelin-1 expression, together with reduced bile production, oxygen consumption, and GSH/GSSG ratio (vs. CT). In the H (+) group, these harmful changes were significantly suppressed [vs. H (-)]. Hydrogen gas reduced hepatic reperfusion injury after prolonged cold preservation via the maintenance of portal flow, by protecting mitochondrial function during the early phase of reperfusion, and via the suppression of oxidative stress and inflammatory cascades thereafter.

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Section: Discussionsupporting

confidence: 91%

Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver

Shimada¹,

Wakayama²,

Fukai

et al. 2016

Artificial Organs

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“…Protective effects with hydrogen rich water (HW) on kidney have been shown in rats with spontaneous hypertension, 3) metabolic syndrome in SHR.Cg-Leprcp/ NDmcr rat kidney, 4) renal ischemia-reperfusion injury, 5) nephrotoxicity induced by ferric nitrilotriacetate, 6) gentamicin 7) or cisplatin, 8) etc. It has also been documented that HW improve metamorphosis and nephrotoxicity induced by reactive oxygen species (ROS) triggered by cisplatin.…”

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confidence: 99%

Hydrogen Rich Water Attenuates Renal Injury and Fibrosis by Regulation Transforming Growth Factor-β Induced Sirt1

Zhou¹,

Pan

Zhang

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Hydrogen-enriched dialysate was prepared using MiZ nondestructive hydrogen dissolver (MiZ, Kanagawa, Japan), as reported elsewhere [11]. When commercial peritoneal dialysate is immersed in H 2 -enriched water, hydrogen permeates through the container, resulting in the H 2 concentration of dialysate gradually increasing in a time-dependent manner (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients: a novel approach to suppress oxidative stress in the peritoneal cavity

et al. 2013

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BackgroundOxidative stress (OS) related to glucose degradation products such as methylglyoxal is reportedly associated with peritoneal deterioration in patients treated with peritoneal dialysis (PD). However, the use of general antioxidant agents is limited due to their harmful effects. This study aimed to clarify the influence of the novel antioxidant molecular hydrogen (H2) on peritoneal OS using albumin redox state as a marker.MethodsEffluent and blood samples of 6 regular PD patients were obtained during the peritoneal equilibrium test using standard dialysate and hydrogen-enriched dialysate. The redox state of albumin in effluent and blood was determined using high-performance liquid chromatography.ResultsMean proportion of reduced albumin (ƒ(HMA)) in effluent was significantly higher in H2-enriched dialysate (62.31 ± 11.10%) than in standard dialysate (54.70 ± 13.08%). Likewise, serum ƒ(HMA) after administration of hydrogen-enriched dialysate (65.75 ± 7.52%) was significantly higher than that after standard dialysate (62.44 ± 7.66%).ConclusionsTrans-peritoneal administration of H2 reduces peritoneal and systemic OS.

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Hydrogen-Rich University of Wisconsin Solution Attenuates Renal Cold Ischemia–Reperfusion Injury

Cited by 51 publications

References 5 publications

Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver

Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver

Hydrogen Rich Water Attenuates Renal Injury and Fibrosis by Regulation Transforming Growth Factor-β Induced Sirt1

Transperitoneal administration of dissolved hydrogen for peritoneal dialysis patients: a novel approach to suppress oxidative stress in the peritoneal cavity

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