Hydrogen-rich water attenuates brain damage and inflammation after traumatic brain injury in rats

Tian, Runfa; Hou, Zhiyong; Hao, Shuyu; Wu, Weichuan; Mao, Xiang; Xue, Tao; Lu, Te‐Ling; Liu, Baiyun

doi:10.1016/j.brainres.2016.01.029

Cited by 45 publications

(31 citation statements)

References 52 publications

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“…Traumatic brain injury (TBI), a major cause of death and disability, also leads to long-term serious neurological impairment in survivors [1,2] . In the United States alone, nearly 1 500 000 people have suffered from TBI, and approximately one third of trauma-related deaths are attributed to TBI [3] , thus making it a serious issue for public health. TBI consists of a primary injury from the immediate mechanical insult and a secondary injury due to the pathology of blood brain barrier (BBB) disruption, inflammatory responses, cell apoptosis, excitotoxic damage, and free radical production, which evolve over minutes to months after the primary injury [4] .…”

Section: Introductionmentioning

confidence: 99%

Sesamin alleviates blood-brain barrier disruption in mice with experimental traumatic brain injury

Liu

Yang

et al. 2017

Acta Pharmacol Sin

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Sesamin, a major lignan of sesame oil, was reported to have neuroprotective effects in several brain injury models. However, its protective action in maintaining blood-brain barrier (BBB) integrity has not been studied. In this study we investigated the effects of sesamin on the BBB in a mouse model of traumatic brain injury (TBI) and explored the underlying mechanisms. Adult male C57BL/6 mice were subjected to a controlled cortical impact (CCI) injury and then received sesamin (30 mg·kg·d, ip). The mice were euthanized on the 1 and 3 days after CCI injury and samples were collected for analysis. Sesamin treatment significantly attenuated CCI-induced brain edema on the 1 and 3 days after the injury, evidenced by the decreases in water content, tissue hemoglobin levels, Evans blue extravasation and AQP4 expression levels in the ipsilateral cortical tissue compared with the vehicle-treated group. Furthermore, sesamin treatment significantly alleviated CCI-induced loss of the tight junction proteins ZO-1 and occludin in the brain tissues. The neuroprotective mechanisms of sesamin were further explored in cultured mouse brain microvascular bEnd.3 cells subjected to biaxial stretch injury (SI). Pretreatment with sesamin (50 μmol/L) significantly alleviated SI-induced loss of ZO-1 in bEnd.3 cells. Furthermore, we revealed that pretreatment with sesamin significantly attenuated SI-induced oxidative stress and early-stage apoptosis in bEnd.3 cells by decreasing the activation of ERK, p-38 and caspase-3. In conclusion, sesamin alleviates BBB disruption at least partly through its anti-oxidative and anti-apoptotic effects on endothelial cells in CCI injury. These findings suggest that sesamin may be a promising potential therapeutic intervention for preventing disruption of the BBB after TBI.

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Section: Introductionmentioning

confidence: 99%

Sesamin alleviates blood-brain barrier disruption in mice with experimental traumatic brain injury

Liu

Yang

et al. 2017

Acta Pharmacol Sin

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“…It is odourless, non‐toxic and easy to use. Hydrogen protects against oxidative stress and inflammation after TBI, but few studies have focused on the effect of hydrogen on apoptosis and nerve regeneration after injury. In the present study, we observed decreased apoptosis and improved neurite regeneration after hydrogen treatment in an in vitro model of TBI.…”

Section: Discussionmentioning

confidence: 99%

“…The medical value of hydrogen in brain injury is gradually being recognized. Studies by ourselves and others have found that hydrogen gas or hydrogen‐rich water improves blood‐brain barrier leakage, relieves brain oedema and inhibits oxidative stress in rat models of TBI . Some studies confirmed that hydrogen suppresses neuronal apoptosis in animal models of other brain injuries (ie septic, anaesthetic and ischaemic brain injuries), but not in models of TBI .…”

Section: Introductionmentioning

confidence: 97%

Hydrogen exerts neuroprotection by activation of the miR‐21/PI3K/AKT/GSK‐3β pathway in an in vitro model of traumatic brain injury

Wang

Yin

Wang

et al. 2020

J Cellular Molecular Medi

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Few studies have explored the effect of hydrogen on neuronal apoptosis or impaired nerve regeneration after traumatic brain injury, and the mechanisms involved in these processes are unclear. In this study, we explored neuroprotection of hydrogen‐rich medium through activation of the miR‐21/PI3K/AKT/GSK‐3β pathway in an in vitro model of traumatic brain injury. Such model adopted PC12 cells with manual scratching. Then, injured cells were cultured in hydrogen‐rich medium for 48 hours. Expression of miR‐21, p‐PI3K, p‐Akt, p‐GSK‐3β, Bax and Bcl‐2 was measured using RT‐qPCR, Western blot analysis and immunofluorescence staining. Rate of apoptosis was determined using TUNEL staining. Neuronal regeneration was assessed using immunofluorescence staining. The results showed that hydrogen‐rich medium improved neurite regeneration and inhibited apoptosis in the injured cells. Scratch injury was accompanied by up‐regulation of miR‐21, p‐PI3K, p‐Akt and p‐GSK‐3β. A miR‐21 antagomir inhibited the expression of these four molecules, while a PI3K blocker only affected the three proteins and not miR‐21. Both the miR‐21 antagomir and PI3K blocker reversed the protective effect of hydrogen. In conclusion, hydrogen exerted a neuroprotective effect against neuronal apoptosis and impaired nerve regeneration through activation of miR‐21/PI3K/AKT/GSK‐3β signalling in this in vitro model of traumatic brain injury.

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“…HRW memiliki kandungan hidrogen sebesar 0,55-0,65 mM (Nakao et al, 2010). HRW dipilih karena harganya murah, mudah didapatkan, tidak mengandung senyawa kimia yang berbahaya dan memiliki manfaat antara lain antikanker dan pencegah alzaimer (Zhao et al, 2016;Tian et al, 2016). Pemanfaatan HRW selama ini hanya digunakan pada bidang kesehatan, belum pernah diaplikasikan untuk bahan pangan.…”

Section: Pendahuluanunclassified

Aplikasi Hydrogen Rich Water Pada Modifikasi Tepung Kentang Dengan Pengering Gelombang Mikro Sebagai Alternatif Substitusi Gandum

Budiarti

Sulistiawati

2019

EKW

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Kentang merupakan umbi tinggi mineral dapat digunakan sebagai bahan alternatif substitusi gandum. Namun, kentang memiliki daya kembang yang rendah dibandingkan gandum. Sehingga dilakukan modifikasi menggunakan hydrogen rich water yang aman untuk makanan dibantu dengan pengering gelombang mikro. Penelitian ini bertujuan untuk mengetahui pengaruh hydrogen rich water terhadap sifat fisikokimia tepung kentang yaitu swelling power, kelarutan, viskositas dan morfologi tepung kentang. Variasi waktu perendaman modifikasi pati dan pH yang digunakan adalah 10, 20, 30, dan 40 menit dan 9, 5. Dari hasil yang diperoleh, perlakuan yang direkomendasikan adalah waktu perendaman 30 menit dan pH 5 dengan nilai swelling power, kelarutan dan viskositas masing-masing 12,51 g/g, 2,28%, dan 1,14 cP. Analisis Scanning Electron Microsopy (SEM) menunjukkan morfologi pati yang telah dimodifikasi kasar dan menyatu. Hasil yang diperoleh lebih tinggi daripada nilai dari tepung terigu. Sehingga kentang dapat dipertimbangkan sebagai bahan alternatif substitusi terigu.

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Hydrogen-rich water attenuates brain damage and inflammation after traumatic brain injury in rats

Cited by 45 publications

References 52 publications

Sesamin alleviates blood-brain barrier disruption in mice with experimental traumatic brain injury

Sesamin alleviates blood-brain barrier disruption in mice with experimental traumatic brain injury

Hydrogen exerts neuroprotection by activation of the miR‐21/PI3K/AKT/GSK‐3β pathway in an in vitro model of traumatic brain injury

Aplikasi Hydrogen Rich Water Pada Modifikasi Tepung Kentang Dengan Pengering Gelombang Mikro Sebagai Alternatif Substitusi Gandum

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