Hydrogen-Rich Water Reestablishes ROS Homeostasis but Exerts Differential Effects on Anthocyanin Synthesis in Two Varieties of Radish Sprouts under UV-A Irradiation

Abstract

The aims of the study were to investigate whether hydrogen gas (H2) was involved in regulation of anthocyanin biosynthesis in two contrasting radish (Raphanus sativus L.) varieties (low [LA] and high [HA] level of anthocyanin) under UV irradiation. The results showed that hydrogen-rich water (HRW) significantly blocked the UV-A-induced increase of H2O2 and O2(•-) accumulation, and enhanced the UV-A-induced increase of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities in LA and HA. Furthermore, UV-A-induced increase of anthocyanin and total phenols was further enhanced only in HA sprouts cotreated with HRW. LC-MS/MS analysis showed that five anthocyanidins existed in HA sprouts, but only two in LA sprouts. Meanwhile, the cyanidin was the most abundant anthocyanidin in HA, and the cyanidin was 2-fold higher cotreated with HRW than UV-A. Molecular analyses showed that the anthocyanin biosynthesis-related genes were upregulated significantly in both HA (in particular) and LA sprouts treated with HRW plus UV-A. These data imply that HRW reestablishes reactive oxygen species homeostasis in both LA and HA, but exerts different effects on anthocyanin accumulation between them under UV-A.

Linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation, water status and reactive oxygen species balance: a case study for rice

“…Similar antioxidant behaviours of exogenous H 2 have been reported in studies of plant tolerance of abiotic stresses (e.g. Xie et al, 2012Xie et al, , 2014Xie et al, , 2015Cui et al, 2013Cui et al, , 2014Jin et al, 2013;Xu et al, 2013;Su et al, 2014).…”

“…Similar to the approach used in animals, hydrogen-rich water was regarded as a safe and easily available means of investigating the physiological function of endogenous H 2 in plants. It has been shown that H 2 might be a novel bioregulator involved in phytohormone signalling , the delay of fruit senescence (Hu et al, 2014) and plant responses to various stresses, including paraquat (Jin et al, 2013), ultraviolet radiation (Su et al, 2014;Xie et al, 2015), drought (Xie et al, 2014), salinity (Xie et al, 2012;Xu et al, 2013), cadmium and mercury exposure (Cui et al, 2014). However, whether H 2 regulates plant adaptive responses to B toxicity is unknown.…”

Protective Effect of Selenium‐Enriched Red Radish Sprouts on Carbon Tetrachloride‐Induced Liver Injury in Mice

“…Therefore, improving the selenium content in food, improving peoples’ daily selenium intake is important. As an industrialized large‐scale vegetable, radish sprouts have gained popularity in East Asian countries, so radish sprouts are an ideal material for selenium‐fortified vegetables (Su et al., ; ). In this study, we treated radish sprouts with Na 2 SeO 3 and Na 2 SeO 4 .…”

Increased Sucrose in the Hypocotyls of Radish Sprouts Contributes to Nitrogen Deficiency-Induced Anthocyanin Accumulation

“…The determination of anthocyanin content in the radish hypocotyls was according to the method developed by Su et al (2014), which involves measuring the absorbance (530) of extracts.…”

“…Primer Expressversion 3.0 (Applied Biosystems) was used to design all PCR primers targeting actin, PAL, CHS, CHI, F3H, DFR, LDOX, ANS , and UFGT (Su et al, 2014). All primers (Supplementary Table 1) were synthesized by Genewiz Bio-engineering Ltd. Company (Suzhou, China).…”