Hydrogen sulfide alleviates cognitive deficiency and hepatic dysfunction in a mouse model of acute liver failure

Abstract

Acute liver failure (ALF) is a devastating clinical syndrome with a high mortality rate if not treated promptly. Previous studies have demonstrated the beneficial effects of hydrogen sulfide (H 2 S) on the brain and liver. The present study aimed to investigate the potential protective effects of H 2 S in ALF. A mouse model of ALF was established following treatment with thioacetamide (TAA). Mice with TAA-induced ALF were intraperitoneally injected with 30 or 100 µmol/kg/day sodium hydrosulfide (NaHS; a H 2 S donor drug) for two weeks. According to results from novel object recognition and Y-maze tests, in the present study, NaHS treatment alleviated cognitive deficiency and preserved spatial orientation learning ability in TAA-induced ALF mice compared with those of untreated mice. In addition, NaHS treatment reduced serum levels of aspartate transaminase (AST), alanine transaminase (ALT) and the concentration of ammonia compared with those that received control treatment, resulting in weight loss prevention. These findings suggested a beneficial effect of H 2 S on liver function. In conclusion, results from the present study suggested that H 2 S treatment may alleviate cognitive deficiency and hepatic dysfunction in mice with ALF, indicating the potential therapeutic benefits of applying H 2 S for the treatment of ALF.

Inhibition of Autotaxin Ameliorates LPA-Mediated Neuroinflammation and Alleviates Neurological Dysfunction in Acute Hepatic Encephalopathy

“…As discussed earlier, HE is characterized by cognitive and motor deficit and change in personality and consciousness . Interestingly, here, ATXi treatment exhibited significant reversal ( p < 0.05) of the spontaneous locomotor distance traveled, rearing, active time, and mobile time with respect to the vehicle group (Figure A–D, respectively).…”

“…As discussed earlier, HE is characterized by cognitive and motor deficit and change in personality and consciousness. 36 Interestingly, here, ATXi treatment exhibited significant reversal (p < 0.05) of the spontaneous locomotor distance traveled, rearing, active time, and mobile time with respect to the vehicle group (Figure 8A−D, respectively). We also observed significant amelioration in the HE-related clinical grading score (Figure 8E) along with improvement in rotarod activity impairment (Figure 8F) compared with the vehicletreated group (Figure 8D).…”

“…The discrimination index was calculated using the formula difference between the time taken to explore new and familiar objects/total time. 36 5.4. Rotarod Test.…”

“…Sniffing and touching the objects were considered as exploratory behaviors. The discrimination index was calculated using the formula difference between the time taken to explore new and familiar objects/total time …”

SCFAs Ameliorate Chronic Postsurgical Pain–Related Cognition Dysfunction via the ACSS2-HDAC2 Axis in Rats

“…The open-field test was applied to assess locomotor activity. The Y-maze test was used to determine the spatial orientation learning memory ability, and the novel object preference test was performed to evaluate the non-spatial visual learning memory [23][24][25]. The test devices were cleaned with 75% ethanol after every trial to avoid the interference of any odor.…”