Hydrogen sulfide attenuates epithelial–mesenchymal transition of human alveolar epithelial cells

Exercise training ameliorates bleomycin‐induced epithelial mesenchymal transition and lung fibrosis through restoration of H2S synthesis

“…However, the mechanism underlying the modulation of CBS/CSE/ H 2 S pathway by exercise training remains to be elucidated. Notably, previous studies have shown that TGF‐β1 significantly inhibits CBS and CSE expression in alveolar epithelial cells and proximal tubular epithelial cells respectively. These findings let us speculate that exercise training may restore pulmonary CBS/CSE expression and the consequent endogenous H 2 S production via suppressing TGF‐β1 production and Smad activation during bleomycin‐induced lung fibrosis.…”

“…As discussed above, EMT has been highlighted as a potential contributor to cellular transdifferentiation from epithelial cells to mesenchymal cells during the development of lung fibrosis . In in vitro studies, H 2 S has been found to inhibit TGF‐β‐driven EMT in human breast cancer cells, alveolar epithelial cells and proximal tubular epithelial cells . H 2 S can also inhibit TGF‐β‐induced increase in β‐catenin expression and nuclear translocation .…”

“…Recent studies further indicate that the cross‐talk between Wnt/β‐catenin and TGF‐β/Smad signalling is critical for the pathogenesis of EMT and pulmonary fibrosis . Notably, it has been reported that H 2 S attenuates TGF‐β induced EMT in alveolar epithelial cells and renal tubular epithelial cells . H 2 S can also inhibit TGF‐β‐induced increase in β‐catenin expression and nuclear translocation .…”

Inhibition of Epithelial-to-Mesenchymal Transition and Pulmonary Fibrosis by Methacycline

“…More recently, inhibitors of EMT that target interactions between Smad and b-catenin signaling have been reported to attenuate fibrosis in vivo (17). A number of other inhibitors of EMT in vitro have been identified, but their effects on fibrogenesis are unknown (36)(37)(38). Recently, inhibition of Akt was shown to suppress EMT and fibrosis in vivo in mice deficient in epithelial phosphatase and tensin homolog (PTEN), which display enhanced epithelial PI3K and Akt activities (20).…”

Chemiluminescent probes for imaging H2S in living animals

“…We therefore examined the ability of CHS-3 to detect cellular H 2 S production in human lung adenocarcinoma epithelial cells (A549). These cells express the enzyme cystathionine γ-lyase (CSE), 51 which can utilize homocysteine (Hcy) as a substrate for H 2 S production. We first demonstrated the ability of CHS-3 to detect exogenous Na 2 S in a multi-well plate reader format ( Fig.…”