Hydrogen sulfide – cysteine cycle plays a positive role in Arabidopsis responses to Copper Oxide nanoparticles stress

Jia, Honglei; Yang, Jun; Liu, Huaxin; Liu, Kena; Ma, Peiyun; Chen, Sisi; Shi, Wei; Wei, Ting; Ren, Xueguang; Guo, Junkang; Li, Jisheng

doi:10.1016/j.envexpbot.2018.06.034

Cited by 23 publications

(12 citation statements)

References 53 publications

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“…The drought tolerance of ost1-3/2.6 C131S and des1 partially decreased, but some plants resumed growth after re-watered for 8 h and 24 h (Figure 7A). Electrolyte leakage (EL) and malondialdehyde (MDA) are considered good indicators of stress-induced cell damage (Jia et al, 2018b). After drought stress, EL and MDA are significantly elevated.…”

Section: Plants Expressing Persulfidation-site Mutants Of Snrk26 Show Decreased Drought Tolerancementioning

confidence: 99%

“…Lipid peroxidation of roots was measured by estimating the MDA content according to the method of Jia et al (2016). Measurement of ion leakage was determined according to this method (Jia et al, 2018b). EL and MDA assays were measured after drought treatment for 18 days.…”

Section: El and Mda Assaysmentioning

confidence: 99%

“…Hydrogen sulfide (H 2 S), a water-soluble and small gasotransmitter, plays an important and positive physiological function in regulating plant adaptation to environmental stress and growth (Jia et al, 2018b;Li et al, 2018). Exogenous application of an H 2 S donor can trigger stomatal closure, whereas application of the H 2 S scavenger hypotaurine inhibits stomatal closure (Jia et al, 2018a), suggesting a positive role of exogenous H 2 S in stomatal closure.…”

Section: Introductionmentioning

confidence: 99%

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Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

et al. 2020

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The phytohormone abscisic acid (ABA) plays pivotal roles in triggering stomatal closure and facilitating adaptation of plants to drought stress. Hydrogen sulfide (H 2 S), a small signaling gas molecule, is involved in ABA-dependent stomatal closure. However, how H 2 S regulates ABA signaling remains largely unclear.Here, we show that ABA induces the production of H 2 S catalyzed by L-CYSTEINE DESULFHYDRASE1 (DES1) in guard cells, and H 2 S in turn positively regulates ABA signaling through persulfidation of Open Stomata 1 (OST1)/SNF1-RELATED PROTEIN KINASE2.6 (SnRK2.6). Two cysteine (Cys) sites, Cys131 and Cys137, which are exposed on the surface of SnRK2.6 and close to the activation loop, were identified to be persulfidated, which promotes the activity of SnRK2.6 and its interaction with ABA response element-binding factor 2 (ABF2), a transcription factor acting downstream of ABA signaling. When Cys131, Cys137, or both residues in SnRK2.6 were substituted with serine (S), H 2 S-induced SnRK2.6 activity and SnRK2.6-ABF2 interaction were partially (SnRK2.6 C131S and SnRK2.6 C137S ) or completely (SnRK2.6 C131SC137S ) compromised. Introduction of SnRK2.6 C131S , SnRK2.6 C137S , or SnRK2.6 C131SC137S into the ost1-3 mutant could not rescue the mutant phenotype: less sensitivity to ABA-and H 2 S-induced stomatal closure and Ca 2+ influx as well as increased water loss and decreased drought tolerance. Taken together, our study reveals a novel post-translational regulatory mechanism of ABA signaling whereby H 2 S persulfidates SnRK2.6 to promote ABA signaling and ABA-induced stomatal closure.

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Section: Plants Expressing Persulfidation-site Mutants Of Snrk26 Show Decreased Drought Tolerancementioning

confidence: 99%

Section: El and Mda Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

et al. 2020

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“…The most effective strategy for plants to cope with the threat of toxic metals is to temporarily “inactivate” the metal ions through GSH phytochelatins (PCs) and metallothionein (MTs), which is closely related to the sulfur metabolism pathway with H 2 S-cysteine as the core. Even without exogenous H 2 S, toxic metal stress can induce the activity of CDes, OAS-TL, CAS and SATs (Cui et al 2014 ; Fang et al 2016 , 2017 ; Jia et al 2016 , 2018b ; Lv et al 2017 ; Talukdar 2015 ; Yu et al 2019 ), resulting in the production of more endogenous H 2 S and cysteine (Jia et al 2016 ; Shi et al 2014 ; Talukdar 2016 ; Zhang et al 2010a ). Cysteine is the raw material for GSH synthesis through γ-CEs synthetase and GSH synthase (Jobe et al 2012 ), and H 2 S can increase the expression of the genes related to PCs and MTs through transcriptional regulation (Fang et al 2014a , 2016 ; Jia et al 2016 ; Liu et al 2016 ; Valivand et al 2019a ).…”

Section: H 2 S Positively Responds To Biotic and Amentioning

confidence: 99%

Hydrogen sulfide (H2S) signaling in plant development and stress responses

et al. 2021

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Hydrogen sulfide (H 2 S) was initially recognized as a toxic gas and its biological functions in mammalian cells have been gradually discovered during the past decades. In the latest decade, numerous studies have revealed that H 2 S has versatile functions in plants as well. In this review, we summarize H 2 S-mediated sulfur metabolic pathways, as well as the progress in the recognition of its biological functions in plant growth and development, particularly its physiological functions in biotic and abiotic stress responses. Besides direct chemical reactions, nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) have complex relationships with H 2 S in plant signaling, both of which mediate protein post-translational modification (PTM) to attack the cysteine residues. We also discuss recent progress in the research on the three types of PTMs and their biological functions in plants. Finally, we propose the relevant issues that need to be addressed in the future research. Graphic abstract Supplementary Information The online version contains supplementary material available at 10.1007/s42994-021-00035-4.

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“…Interestingly, Jia et al found that the Cys cycle affected the uptake and intracellular transport of CuO NPs. The activity and toxicity of CuO NPs were reduced by promoting the production of chelators while stabilizing the level of CuO NP-induced reactive oxygen species [69].…”

Section: Toxic Effects At the Physiological Levelmentioning

confidence: 99%

Recent Advances in Metal-Based Nanoparticle-Mediated Biological Effects in Arabidopsis thaliana: A Mini Review

Geng

et al. 2022

Materials

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The widespread application of metal-based nanoparticles (MNPs) has prompted great interest in nano-biosafety. Consequently, as more and more MNPs are released into the environment and eventually sink into the soil, plants, as an essential component of the ecosystem, are at greater risk of exposure and response to these MNPs. Therefore, to understand the potential impact of nanoparticles on the environment, their effects should be thoroughly investigated. Arabidopsis (Arabidopsis thaliana L.) is an ideal model plant for studying the impact of environmental stress on plants’ growth and development because the ways in which Arabidopsis adapt to these stresses resemble those of many plants, and therefore, conclusions obtained from these scientific studies have often been used as the universal reference for other plants. This study reviewed the main findings of present-day interactions between MNPs and Arabidopsis thaliana from plant internalization to phytotoxic effects to reveal the mechanisms by which nanomaterials affect plant growth and development. We also analyzed the remaining unsolved problems in this field and provide a perspective for future research directions.

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Hydrogen sulfide – cysteine cycle plays a positive role in Arabidopsis responses to Copper Oxide nanoparticles stress

Cited by 23 publications

References 53 publications

Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

Hydrogen sulfide (H2S) signaling in plant development and stress responses

Recent Advances in Metal-Based Nanoparticle-Mediated Biological Effects in Arabidopsis thaliana: A Mini Review

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