2017
DOI: 10.1002/cre2.69
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Hydrogen sulfide exposure induces NLRP3 inflammasome‐dependent IL‐1β and IL‐18 secretion in human mononuclear leukocytes in vitro

Abstract: The aim was to investigate if hydrogen sulfide (H2S) induces the formation of the NLRP3 inflammasome and subsequent IL‐1β and IL‐18 secretion in human peripheral blood mononuclear cells (PBMCs) and in the human monocyte cell line THP1. Bacterial production of H2S has been suggested to participate in the inflammatory host response in periodontitis pathogenesis. H2S is a toxic gas with pro‐inflammatory properties. It is produced by bacterial degradation of sulfur‐containing amino acids, for example, cysteine. We… Show more

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Cited by 26 publications
(28 citation statements)
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“…When the cells were exposed to H 2 S, the secretion of IL-1β and IL-18 was increased from the cells of the majority of the subjects in both groups. These results are in accordance with those of our previous in vitro study of unknown blood donors [13] and confirm that H 2 S induces PBMCs to secrete proinflammatory cytokines. The level of secretion was, however, highly variable between the individuals tested, and some individuals (N = 9, IL-1β and N = 4, IL-18) actually secreted lower levels of IL-1β and IL-18 after H 2 S exposure than unexposed control cells.…”
Section: Discussionsupporting
confidence: 93%
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“…When the cells were exposed to H 2 S, the secretion of IL-1β and IL-18 was increased from the cells of the majority of the subjects in both groups. These results are in accordance with those of our previous in vitro study of unknown blood donors [13] and confirm that H 2 S induces PBMCs to secrete proinflammatory cytokines. The level of secretion was, however, highly variable between the individuals tested, and some individuals (N = 9, IL-1β and N = 4, IL-18) actually secreted lower levels of IL-1β and IL-18 after H 2 S exposure than unexposed control cells.…”
Section: Discussionsupporting
confidence: 93%
“…The blood samples were analyzed for C-reactive protein (CRP), and transferrin at the Clinical Chemistry Laboratory of Sahlgrenska University Hospital, Gothenburg, Sweden. PBMCs were isolated and exposed to a hydrogen sulfide generator as previously described [13]. Briefly, PBMCs were isolated by centrifugation with Ficoll-Paque TM Plus (GE Healthcare Bio-Sciences AB, Uppsala, Sweden), washed with PBS, and resuspended in Dulbecco's Modified Eagle Medium plus GlutaMAX TM (Gibco, Life Technologies, Paisley, UK) supplemented with 5% human serum (Sigma-Aldrich) and penicillin-streptomycin (Sigma-Aldrich).…”
Section: Blood Samplesmentioning
confidence: 99%
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“…Helicobacter pylori predominantly activates the NLRP3 inflammasome through a process that requires TLR2‐dependent action (Koch & Muller, ). Sulphated bacterial products enhance NLRP3 inflammasome‐dependent cytokine secretion by human mononuclear leucocytes (Basic, Alizadehgharib, Dahlen, & Dahlgren, ), and dental calculus stimulates IL‐1β secretion via NLRP3 inflammasome in macrophages (Montenegro Raudales et al., ). Further, Td92, a surface protein of T. denticola activated NLRP3 in macrophages (Jun, Lee, Lee, & Choi, ) and bone matrix components, activates the NLRP3 inflammasome and promotes osteoclast differentiation (Alippe et al., ).…”
Section: Preamblementioning
confidence: 99%