Hydrogen sulfide improves tall fescue photosynthesis response to low-light stress by regulating chlorophyll and carotenoid metabolisms

Liu, Bowen; Zhang, Xu-hu; You, Xiangkai; Li, Youyue; Long, Si; Wen, Suyun; Liu, Qian; Liu, Tieyuan; Guo, Huan; Xu, Yuefei

doi:10.1016/j.plaphy.2021.12.002

Cited by 29 publications

(11 citation statements)

References 71 publications

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“…A mixed-liquid-extraction method was adopted for the determination, conducted in accordance with the previous report [ 63 ]. Briefly, the mixed extract was absolute (ethanol: acetone: distilled water = 4.5:4.5:1), and the OD values were obtained at the wavelength of 440 nm, 645 nm, and 663 nm.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic Analysis for Diurnal Temperature Differences Reveals Gene-Regulation-Network Response to Accumulation of Bioactive Ingredients of Protocorm-like Bodies in Dendrobium officinale

Chen,

Zhang,

Chen

et al. 2024

Plants

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Dendrobium officinale Kimura et Migo (D. officinale) is one of the most important traditional Chinese medicinal herbs, celebrated for its abundant bioactive ingredients. This study demonstrated that the diurnal temperature difference (DIF) (T1: 13/13 °C, T2: 25/13 °C, and T3: 25/25 °C) was more favorable for high chlorophyll, increased polysaccharide, and total flavonoid contents compared to constant temperature treatments in D. officinale PLBs. The transcriptome analysis revealed 4251, 4404, and 4536 differentially expressed genes (DEGs) in three different comparisons (A: 25/13 °C vs. 13/13 °C, B: 13/13 °C vs. 25/25 °C, and C: 25/13 °C vs. 25/25 °C, respectively). The corresponding up-/down-regulated DEGs were 1562/2689, 2825/1579, and 2310/2226, respectively. GO and KEGG enrichment analyses of DEGs showed that the pathways of biosynthesis of secondary metabolites, carotenoid biosynthesis, and flavonoid biosynthesis were enriched in the top 20; further analysis of the sugar- and flavonol-metabolism pathways in D. officinale PLBs revealed that the DIF led to a differential gene expression in the enzymes linked to sugar metabolism, as well as to flavonol metabolism. Certain key metabolic genes related to ingredient accumulation were identified, including those involved in polysaccharide metabolism (SUS, SUT, HKL1, HGL, AMY1, and SS3) and flavonol (UGT73C and UGT73D) metabolism. Therefore, these findings indicated that these genes may play an important role in the regulatory network of the DIF in the functional metabolites of D. officinale PLBs. In a MapMan annotation of abiotic stress pathways, the DEGs with significant changes in their expression levels were mainly concentrated in the heat-stress pathways, including heat-shock proteins (HSPs) and heat-shock transcription factors (HSFs). In particular, the expression levels of HSP18.2, HSP70, and HSF1 were significantly increased under DIF treatment, which suggested that HSF1, HSP70 and HSP18.2 may respond to the DIF. In addition, they can be used as candidate genes to study the effect of the DIF on the PLBs of D. officinale. The results of our qPCR analysis are consistent with those of the transcriptome-expression analysis, indicating the reliability of the sequencing. The results of this study revealed the transcriptome mechanism of the DIF on the accumulation of the functional metabolic components of D. officinale. Furthermore, they also provide an important theoretical basis for improving the quality of D. officinale via the DIF in production.

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Section: Methodsmentioning

confidence: 99%

Transcriptomic Analysis for Diurnal Temperature Differences Reveals Gene-Regulation-Network Response to Accumulation of Bioactive Ingredients of Protocorm-like Bodies in Dendrobium officinale

Chen,

Zhang,

Chen

et al. 2024

Plants

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“…The following six treatments were applied: (1) ddH 2 O (Control, CK), (2) ddH 2 O under LT stress (LT), (3) 500 μM NaHS (H 2 S donor) under LT stress (LT + H 2 S), (4) 300 μM hypotaurine (HT, H 2 S scavenger) under LT stress (LT + HT), (5) 300 μM hydroxylamine (HA, H 2 S inhibitor) under LT stress (LT + HA), (6) 300 μM hydroxylamine (HA, H 2 S inhibitor) and 500 μM NaHS under LT stress (LT + HT + H 2 S). The chemicals were sprayed on the leaves three times per week (Liu et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…After 5 weeks (Kareem et al, 2022) The chemicals were sprayed on the leaves three times per week (Liu et al, 2022).…”

Section: Plant Growth and Experimental Designmentioning

confidence: 99%

Integrated bioinformatic and physiological analyses reveal the pivotal role of hydrogen sulfide in enhancing low‐temperature tolerance in alfalfa

Gao

Wang

Zeng

et al. 2023

Physiologia Plantarum

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Hydrogen sulfide (H2S) is an important gaseous signal molecule that regulates plant growth and stress resistance. However, research on the H2S synthase (HSase) genes is still limited in the model legume plant Medicago truncatula Gaertn. In the present study, a total of 40 HSase family members were first identified and analyzed in the M. truncatula genome, and these genes distributed across eight chromosomes and were clustered into five groups (I–V) based on their conserved gene structures and protein motifs. Expression analysis revealed that the MtHSase genes were expressed in all the tested abiotic stresses, albeit with expression level differences. This study also showed that H2S improves low temperature tolerance of alfalfa seedlings by regulating the antioxidant defense system and enhancing photosynthetic capacity. Thus, the study provides new insights into how the H2S signal regulates tolerance to low‐temperature stress and provides the basis for further gene function and detection.

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“…In this case, each treatment was replicated thrice. During the 14 d treatment, the PPFD above the plants was 40 μmol m −2 s −1 , according to our previous research on different low-light intensity ranges [ 58 , 59 ], and the PBZ was sprayed four times. According to the comprehensive results of the morphology, the total chlorophyll content, and the MDA content, the optimal PBZ concentration was identified ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Paclobutrazol Ameliorates Low-Light-Induced Damage by Improving Photosynthesis, Antioxidant Defense System, and Regulating Hormone Levels in Tall Fescue

Liu

Long

Liu

et al. 2022

IJMS

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Paclobutrazol (PBZ) is a plant-growth regulator (PGR) in the triazole family that enhances plant tolerance to environmental stresses. Low-light (LL) intensity is a critical factor adversely affecting the growth of tall fescue (Festuca arundinacea Schreb.). Therefore, in this study, tall fescue seedlings were treated with PBZ under control and LL conditions to investigate the effects of PBZ on enhancing LL stress resistance by regulating the growth, photosynthesis, oxidative defense, and hormone levels. Our results reveal that LL stress reduced the total biomass, chlorophyll (Chl) content, photosynthetic capacity, and photochemical efficiency of photosystem II (PSII) but increased the membrane lipid peroxidation level and reactive oxygen species (ROS) generation. However, the application of PBZ increased the photosynthetic pigment contents, net photosynthetic rate (Pn), maximum quantum yield of PSII photochemistry (Fv/Fm), ribulose-1,5-bisphosphate carboxylase (RuBisCO) activity, and starch content. In addition, PBZ treatment activated the antioxidant enzyme activities, antioxidants contents, ascorbate acid-glutathione (AsA-GSH) cycle, and related gene expression, lessening the ROS burst (H2O2 and O2∙−). However, the gibberellic acid (GA) anabolism was remarkably decreased by PBZ treatment under LL stress, downregulating the transcript levels of kaurene oxidase (KO), kaurenoic acid oxidase (KAO), and GA 20-oxidases (GA20ox). At the same time, PBZ treatment up-regulated 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression, significantly increasing the endogenous abscisic acid (ABA) concentration under LL stress. Thus, our study revealed that PBZ improves the antioxidation and photosynthetic capacity, meanwhile increasing the ABA concentration and decreasing GA concentration, which ultimately enhances the LL stress tolerance in tall fescue.

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Hydrogen sulfide improves tall fescue photosynthesis response to low-light stress by regulating chlorophyll and carotenoid metabolisms

Cited by 29 publications

References 71 publications

Transcriptomic Analysis for Diurnal Temperature Differences Reveals Gene-Regulation-Network Response to Accumulation of Bioactive Ingredients of Protocorm-like Bodies in Dendrobium officinale

Transcriptomic Analysis for Diurnal Temperature Differences Reveals Gene-Regulation-Network Response to Accumulation of Bioactive Ingredients of Protocorm-like Bodies in Dendrobium officinale

Integrated bioinformatic and physiological analyses reveal the pivotal role of hydrogen sulfide in enhancing low‐temperature tolerance in alfalfa

Paclobutrazol Ameliorates Low-Light-Induced Damage by Improving Photosynthesis, Antioxidant Defense System, and Regulating Hormone Levels in Tall Fescue

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