Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia

Wang, Shuang; Chi, Qianru; Hu, Xueyuan; Cong, Yingzi

doi:10.1016/j.ecoenv.2019.109578

Cited by 78 publications

(28 citation statements)

References 35 publications

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“…Bax is considered an apoptosis-related and inflammation-related protein [31,32]. Upregulation of cleaved caspase-3 could clearly induce apoptosis in cancer cells [33].…”

Section: Discussionmentioning

confidence: 99%

miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy

Qiu

Xia

et al. 2021

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Liver cancer is the sixth most prevalent cancer worldwide and the third leading cause of cancer-related deaths. Adriamycin (ADR) resistance, which often leads to the progression of malignant tumors, is a major treatment obstacle for liver cancer. It has been confirmed that miR-155-5p could reverse drug resistance in human breast cancer. However, the biological function of miR-155-5p in ADR-resistant liver carcinoma (HepG2/ADR) cells remains unclear. miR-155-5p and ATG5 expression was determined by RT-qPCR and western blot. In addition, MTT, flow cytometry, immunofluorescence staining, and western blotting were performed to evaluate the proliferation, apoptosis, and autophagy of liver cancer cells. Finally, the effect of miR-155-5p on the expression of autophagy-related 5 (ATG5) was analyzed by luciferase activity assay, western blot, and RT-qPCR. Our results showed that miR-155-5p was downregulated in HepG2/ADR cells. Increasing the expression of miR-155-5p enhanced the sensitivity of liver carcinoma cells to ADR and promoted apoptosis through inhibition of autophagy in vitro. In addition, the binding site between miR-155-5p and ATG5 was identified, and miR-155-5p could directly regulate ATG5. Finally, ATG5 partially rescued the effect of miR-155-5p on autophagy and the apoptosis of HepG2/ADR cells. In conclusion, our findings showed that miR-155-5p could reverse ADR resistance in liver cancer by targeting ATG5, which may function as a potential target for liver cancer treatment.

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“…Bax is considered an apoptosis-related and inflammation-related protein [31,32]. Upregulation of cleaved caspase-3 could clearly induce apoptosis in cancer cells [33].…”

Section: Discussionmentioning

confidence: 99%

miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy

Qiu

Xia

et al. 2021

neo

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“…The level of SOD activity reflects the ability of antifree radicals. SOD can convert superoxide anion (O2 -) into H 2 O 2 , and CAT converts H 2 O 2 into water such that toxic O 2 and H 2 O 2 are converted into harmless water molecules [32,33]. GSH is catalyzed to convert to oxidized glutathione (GSSH) with the assistance of GSH-Px, which can reduce oxidized substances and relieve their toxicity [34,35].…”

Section: Discussionmentioning

confidence: 99%

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart

Gong

Yang

Liu

et al. 2019

Oxidative Medicine and Cellular Longevity

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Insulin-like growth factor 1 (IGF1) is a multifunctional cellular regulatory factor that can regulate cell growth and development by mediating growth hormone stimulation. However, the mechanism of IGF1 dysfunction in cardiomyocyte development is seldom reported. To study this, we employed the models of IGF1 knockdown in chicken embryo in vivo and in cardiomyocytes in vitro. We detected the antioxidant capacity, PI3K/Akt pathway, energy metabolism-related genes, and myocardial development-related genes. Our results revealed that the low expression of IGF1 can significantly suppress the antioxidant capacity and increase the ROS (P<0.05) levels, activating the AMPK and PI3K pathway by inhibiting the expression of IRS1. We also found that myocardial energy metabolism is blocked through IGF1, GLUT, and IGFBP inhibition, further inducing myocardial developmental disorder by inhibiting Mesp1, GATA, Nkx2.5, and MyoD expression. Altogether, we conclude that low IGF1 expression can hinder myocardial development through the dysfunction of energy metabolism caused by ROS-dependent FOXO activation.

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“…A previous study revealed that H 2 S induced oxidative stress in the chicken trachea, indicated by increased levels of hydrogen peroxide (H 2 O 2 ) and MDA, and decreased SOD, GSH, and CAT activity, which resulted in inflammatory injury mediated by FOS/IL8 signaling (Chen et al, 2019). Likewise, H 2 S decreased CAT and total antioxidant capacity (T-AOC) activities and increased H 2 O 2 and nitric oxide content in broilers' myocardial region and in the bursa (Hu et al, 2018;Wang et al, 2019b). Chi et al (2018) suggested that H 2 S triggers inflammatory responses and oxidative stress by enhancing ROS production, then impairing energy metabolism and immune system in the chickens.…”

Section: Environmental Pollutantsmentioning

confidence: 99%

Environmental Stress in Chickens and the Potential Effectiveness of Dietary Vitamin Supplementation

Akinyemi

Adewole

2021

Front. Anim. Sci.

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Environmental stressors can promote the vulnerability of animals to infections; it is therefore, essential to understand how stressors affect the immune system, the adaptive capacity of animals to respond, and effective techniques in managing stress. This review highlights scientific evidence regarding environmental stress challenge models and the potential effectiveness of vitamin supplementation. The major environmental stressors discussed are heat and cold stress, feed restriction, stocking density, and pollutants. Much work has been done to identify the effects of environmental stress in broilers and layers, while few involved other types of poultry. Studies indicated that chickens' performance, health, and welfare are compromised when challenged with environmental stress. These stressors result in physiological alterations, behavioral changes, decreased egg and meat quality, tissue and intestinal damage, and high mortalities. The application of vitamins with other nutritional approaches can help in combating these environmental stressors in chickens. Poultry birds do not synthesize sufficient vitamins during stressful periods. It is therefore suggested that chicken diets are supplemented with vitamins when subjected to environmental stress. Combination of vitamins are considered more efficient than the use of individual vitamins in alleviating environmental stress in chickens.

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Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia

Cited by 78 publications

References 35 publications

miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy

miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart

Environmental Stress in Chickens and the Potential Effectiveness of Dietary Vitamin Supplementation

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