Hydrogen sulfide inhalation-induced immune damage is involved in oxidative stress, inflammation, apoptosis and the Th1/Th2 imbalance in broiler bursa of Fabricius

Hu, Xueyuan; Chi, Qianru; Wang, Dongxu; Chi, Xin; Teng, Xiaohua; Li, Shu

doi:10.1016/j.ecoenv.2018.08.029

Cited by 87 publications

(27 citation statements)

References 46 publications

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“…Total RNA was isolated from PBMCs for determination of the mRNA expression level using SuperScript™ FirstStrand Synthesis System reagent (Thermo Fisher, Waltham, MA, USA) according to the manufacturer's protocol. The method of determination of total RNA purity, cDNA synthesis, and qPCR analysis was slightly modified from Hu et al [20]. Briefly, 2× SYBR GREEN PCR Master Mix-ROX (Gunster Biotech, Co., Ltd., Taipei, Taiwan), cDNA, deionized water, and each primer were mixed at a ratio of 5:1.2:1.8:1.…”

Section: Total Rna Isolation and Qpcrmentioning

confidence: 99%

Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers

Chuang

Lin

Hsieh

et al. 2019

Animals

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Simple Summary: The stress and anti-nutrient effect caused by environmental problems and animal feed is an urgent problem in poultry production. As ancient probiotics, Aspergillus oryzae and Saccharomyces cerevisiae can effectively improve the immunity of animals. Furthermore, the anti-nutrient object, phytate, reduces nutrition absorption. Therefore, S. cerevisiae or A. oryzae with phytase co-fermentation may help solve these problems. Results show that the addition of a fermentation product can effectively reduce the inflammatory response and drop the number of harmful bacteria in the ileum of broilers. Among them, A. oryzae fermentation product has a better effect than S. cerevisiae fermentation product.Abstract: Saccharomyces cerevisiae and Aspergillus oryzae are both ancient probiotic species traditionally used as microbes for brewing beer and soy sauce, respectively. This study investigated the effect of adding these two probiotics with phytase fermentation products to the broilers diet. Fermented products possess protease and cellulase, and the activities were 777.1 and 189.5 U/g dry matter (DM) on S. cerevisiae fermented products (SCFP) and 190 and 213.4 U/g DM on A. oryzae fermented products (AOFP), respectively. Liposaccharides stimulated PBMCs to produce nitric oxide to 120 µmol. Both SCFP and AOFP reduced lipopolysaccharides stimulated peripheral blood mononuclear cells (PBMCs) nitric oxide release to 40 and 60 µmol, respectively. Nevertheless, in an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, SCFP and AOFP also increased the survival rate of lipopolysaccharides stimulated PBMCs by almost two-fold compared to the negative control. A total of 240 broilers were divided into four groups as Control, SCFP 0.1% (SCFP), SCFP 0.05% + AOFP 0.05% (SAFP), and AOFP 0.1% (AOFP) groups, respectively. Each group had 20 broilers, and three replicate pens. The results showed that the addition of SCFP, SAFP, and AOFP groups did not affect the growth performances, but increased the jejunum value of villus height and villus: crypt ratio on SAFP and AOFP groups compared to the control and SCFP groups. Furthermore, adding SCFP, SAFP, and AOFP significantly reduced the number of Clostridium perfringens in ileum chyme. SCFP, SAFP, and AOFP significantly reduced the amount of interleukin-1β, inducible nitric oxide synthases, interferon-γ, and nuclear factor kappa B mRNA expression in PBMCs, especially in the AOFP group. In summary, all the SCFP, SAFP, and AOFP groups can be suggested as a functional feed additive since they enhanced villus: crypt ratio and decreased inflammation-related mRNA expression, especially for AOFP group in broilers.

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Section: Total Rna Isolation and Qpcrmentioning

confidence: 99%

Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers

Chuang

Lin

Hsieh

et al. 2019

Animals

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“…Many studies show that NH 3 and H 2 S adversely affect bird performance including growth rate, feed efficiency, carcass quality and susceptibility to diseases [175,176]. High concentrations of these gases also impair the nervous, respiratory and the cardiovascular system and affect animal behavior [175][176][177][178]. It is noteworthy that the avian respiratory system is unique among vertebrates, and that, consequently, research in mammals including humans cannot be directly applied to poultry [175].…”

Section: Impact Of Ammonia and Hydrogen Sulfide On The Immune System mentioning

confidence: 99%

“…H 2 S also exhibits profound effects on the immune system. Similarly to NH 3 , 20 or 30 ppm H 2 S activated inflammatory responses due to an increase in pro-inflammatory cytokines like TNF-α, IFN-γ, IL-6, IL-8 and IL-17 and a decrease in anti-inflammatory cytokines like IL-2, IL-4 and IL-10 in blood, spleen, and the bursa of Fabricius [177,178,192]. Hu et al [178] additionally reported higher mRNA expressions of IgY, IgM and IgA in the bursa of Fabricius.…”

Section: Impact Of Ammonia and Hydrogen Sulfide On The Immune System mentioning

confidence: 99%

“…Both gases, 60 ppm NH 3 or 20 ppm H 2 S, decreased the relative weight of bursa, spleen and thymus [172,181] and resulted in nuclear debris in the bursa of Fabricius and thymus [178,191]. Only a few studies found no influence of NH 3 on immune parameters like antibody concentrations and cytokine expression after exposing laying hens to 30 ppm NH 3 compared to fresh air [184] or on lymphoid organ weights after exposing broilers to up to 52 ppm NH 3 compared to fresh air [186] or 70 ppm compared to 30 ppm NH 3 [185].…”

Section: Impact Of Ammonia and Hydrogen Sulfide On The Immune System mentioning

confidence: 99%

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Impact of Housing Environment on the Immune System in Chickens: A Review

Hofmann

Schmucker

Bessei

et al. 2020

Animals

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During their lifespan, chickens are confronted with a wide range of acute and chronic stressors in their housing environment that may threaten their welfare and health by modulating the immune system. Especially chronic stressful conditions can exceed the individual’s allostatic load, with negative consequences for immunity. A fully functional immune system is mandatory for health and welfare and, consequently, also for high productivity and safe animal products. This review provides a comprehensive overview of the impact of housing form, light regime as well as aerial ammonia and hydrogen sulfide concentrations on the immune system in chickens. Certain housing conditions are clearly associated with immunological alterations which potentially impair the success of vaccinations or affect disease susceptibility. Such poor conditions counteract sustainable poultry production. This review also outlines current knowledge gaps and provides recommendations for future research.

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“…B-lymphocytes then migrate to peripheral lymphoid organs to colonize and orchestrate functional immune responses that fight against infections (17), and release proinflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukein-1 β (IL-1β), IL-6, IL-8, and IL-4 to develop effective inflammatory responses (18). It has been reported that the increase level of these cytokines could affect Th1/Th2 ratio and impair the function of antigen presenting cells leading to immune disorder (19). Researchers reported that MG-mediated inflammatory responses in airway passages and lungs of chickens, but it is still unclear whether MG-infection alters cytokines expression in chicken BOF and affect its normal functioning.…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma gallisepticum Infection Impaired the Structural Integrity and Immune Function of Bursa of Fabricius in Chicken: Implication of Oxidative Stress and Apoptosis

et al. 2020

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Mycoplasma gallisepticum (MG) induces a dysregulated immune response in the lungs and air ways of poultry. However, the mechanism of MG-induced immune dysregulation is still not completely understood. In the present study, the effect of MG-infection on chicken bursa of fabricius (BOF) is investigated. Histopathology, electron microscopy, TUNEL assay, qRT-PCR and western blot were employed to examine the hallmarks of oxidative stress and apoptosis. The data revealed that MG-infection induced oxidative stress and decreased antioxidant responses in BOF tissues compared to control group. Histopathological study showed pathological changes including reduction in lymphocytes and increased inflammatory cell infiltration in MG-infection group. Ultrastructural assessment represents obvious signs of apoptosis such as mitochondrial swelling, shrinkage of nuclear membrane and fragmentation of nucleus. Increased cytokine activities were observed in MG-infection group compared to control group. Meanwhile, the mRNA and protein expression level of apoptosis-related genes were significantly (p < 0.05) upregulated in MG-infection group. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay further confirmed that MG induced apoptosis in BOF tissues as TUNEL-stained positive nuclei were remarkably increased in MG-infection group. In addition, MG-infection significantly reduced the number of CD8 + lymphocytes in chicken BOF at day 7. Moreover, bacterial load significantly increased at day 3 and day 7 in MG-infection group compared to control group. These results suggested that MG-infection impaired the structural integrity, induced oxidative stress and apoptosis in chicken BOF tissues, which could be the possible causes of damage to immune function in chicken BOF.

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Hydrogen sulfide inhalation-induced immune damage is involved in oxidative stress, inflammation, apoptosis and the Th1/Th2 imbalance in broiler bursa of Fabricius

Cited by 87 publications

References 46 publications

Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers

Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers

Impact of Housing Environment on the Immune System in Chickens: A Review

Mycoplasma gallisepticum Infection Impaired the Structural Integrity and Immune Function of Bursa of Fabricius in Chicken: Implication of Oxidative Stress and Apoptosis

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