Hydrogen sulfide is a regulator of mammary gland development in prepubescent female mice

Zhang, Jing; Ye, Jiayi; Yuan, Cong; Fu, Qin; Zhang, Fenglin; Zhu, Xiaotong; Wang, Lina; Gao, Peng; Shu, Gang; Wang, Songbo; Qiang, Liu; Jiang, Qingyan

doi:10.3892/mmr.2020.11462

Cited by 4 publications

(3 citation statements)

References 46 publications

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“…Previous studies have shown that deficiency of the CTH gene increases the sensitivity of mice to LPS-induced inflammation, as evidenced by the higher expression and secretion of interleukin-2 (IL-2), interleukin-4 (IL-4), IL-6, and TNF-α in CTH-KO mice compared to wild-type mice [ 44 ]. H 2 S may also exert bidirectional effects on mammary gland ductal development, promoting ductal development at low concentrations but inhibiting it at high concentrations [ 45 , 46 ]. As well-known inflammatory cytokines or chemokines, TNF-α , IL-1β , IL-6 , and IL-8 are involved in various types of inflammatory responses and are crucial in inflammatory processes during bacterial infection in bovine mammary glands [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Zhang

Lin

Bai

et al. 2022

Animals

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H2S plays an important role in various inflammatory diseases. However, the role of H2S and synthetic enzymes in Holstein cows with CM is unknown. The aim of this study was to identify DEPs associated with sulfide metabolism and further investigate their roles in dairy cows with CM. From 3739 DEPs generated by data-independent acquisition proteomics, we identified a total of 17 DEPs included in 44 GO terms and five KEGG pathways related to sulfide metabolism, including CTH and cystathionine-β-synthase (CBS). Immunohistochemical and immunofluorescence staining results showed that CTH and CBS proteins were present mainly in the cytoplasm of mammary epithelial cells. Endogenous H2S production in the serum of the CM group was significantly lower than that of the healthy Holstein cows. CTH and CBS mRNA and protein levels in the mammary glands of the CM group were significantly downregulated compared to those of the healthy group. These results indicate that CTH and H2S were correlated with the occurrence and development of CM in Holstein cows, which provides important insights into the function and regulatory mechanism of CTH/H2S in Holstein cows.

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Section: Discussionmentioning

confidence: 99%

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Zhang

Lin

Bai

et al. 2022

Animals

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“…Activation of the mTOR pathway is essential for mammary gland development and lactation, and PRL also exerts a regulatory effect on the mammary gland directly through mTOR [ 54 , 55 ]. A previous study suggested that the treatment with SB-6995551, a 5-HT 5A receptor blocker, may affect the proliferative capacity of human breast tumour cell lines via the mTOR pathway [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line

Dong¹,

Liu²,

Miao³

et al. 2022

J Anim Sci Technol

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5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT.

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“…Given the importance of calcium in many biological functions, adequate calcium intake may reduce the risks of many diseases, such as osteoporosis, [ 2 ] hypertension, [ 3 ] and obesity. [ 4 ] Numerous calcium‐fortified supplements and foods have been marketed to combat calcium deficiency. Nevertheless, calcium deficiency remains a global health concern.…”

Section: Introductionmentioning

confidence: 99%

KFP‐1, a Novel Calcium‐Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels

Chang

Chen

et al. 2021

Molecular Nutrition Food Res

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Introduction Kefir is an acidic and alcoholic fermented milk product with multiple health‐promoting benefits. A previous study demonstrated that kefir enhanced calcium absorption in intestinal Caco‐2 cells. In this study, kefir‐fermented peptide‐1 (KFP‐1) is isolated from the kefir peptide fraction, and its function as a calcium‐binding peptide is characterized. Methods and Results KFP‐1 was identified as a 17‐residue peptide with a sequence identical to that of κ‐casein (residues 138–154) in milk protein. KFP‐1 is demonstrated to promote calcium influx in Caco‐2 and IEC‐6 small intestinal cells in a concentration‐dependent manner. TRPV6, but not L‐type voltage‐gated calcium channels, is associated with the calcium influx induced by KFP‐1. An in vitro calcium binding assay indicates that the full‐length KFP‐1 peptide has a higher calcium‐binding capacity than the two truncated KFP‐1 peptides, KFP‐1∆C5 and KFP‐1C5. Alexa Fluor 594 labeling shows that KFP‐1 is taken up by Caco‐2 cells and interacts with calcium ions and TRPV6 protein. Moreover, KFP‐1 is found moderately resistant to pepsin and pancreatin digestions and enhanced calcium uptake by intestinal enterocytes in vivo. Conclusion These data suggest that KFP‐1, a novel calcium‐binding peptide, binds extracellular calcium ions and enters Caco‐2 and IEC‐6 cells, and promotes calcium uptake through TRPV6 calcium channels. The present study is of great importance for developing kefir‐derived metal ion‐binding peptides as functional nutraceutical additives.

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Hydrogen sulfide is a regulator of mammary gland development in prepubescent female mice

Cited by 4 publications

References 46 publications

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line

KFP‐1, a Novel Calcium‐Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels

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