Hydrogen Sulfide Regulates Inward-Rectifying K+ Channels in Conjunction with Stomatal Closure

Papanatsiou, Maria; Scuffi, Denisse; Blatt, Michael R.; Garcı́a-Mata, Carlos

doi:10.1104/pp.114.256057

Cited by 103 publications

(67 citation statements)

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“…Figure 4 presents the current traces and average steady-state currents as a function of voltage (currentvoltage curves) from guard cells of the Arabidopsis lines. In each case, the currents yielded gating characteristics similar to previous reports (Garcia-Mata et al, 2003;Eisenach et al, 2012;Papanatsiou et al, 2015), albeit with some notable differences in the tmm1 plants. Voltages positive of 240 mV were marked by strong outward-directed currents, with the current amplitude increasing with increasing voltages, while shifting the voltages to more negative values than 2100 mV generated inward-directed currents of increasing amplitude.…”

Section: Stomatal Clustering Influences Ion Transport In Guard Cellssupporting

confidence: 67%

“…4 below) and determined the maximum conductance and gating characteristics of the K + channels (Table II). Jointly fitted curves minimize the number of free parameters and allow comparisons between fitting with common parameter values (Honsbein et al, 2009;Wang et al, 2012;Papanatsiou et al, 2015). For I KOUT and I KIN , this analysis yielded statistically and visually satisfactory fittings with the gating charge held in common to give values of 1.9 6 0.3 and 2.1 6 0.3, respectively.…”

Section: Stomatal Clustering Influences Ion Transport In Guard Cellsmentioning

confidence: 94%

“…To directly assess the impact of stomatal clustering on stomatal closing, we measured stomatal apertures after treatments with the well-established closing stimuli Ca 2+ and abscisic acid (ABA; Grabov and Blatt, 1998;Eisenach et al, 2012;Papanatsiou et al, 2015). Stomata of wild-type and PTMM1 plants closed to similar extents, whereas stomata of the tmm1 mutant Table I.…”

Section: Spacing Between Stomata Is Essential For Stomatal Closurementioning

confidence: 99%

“…When the tmm1 plants were transferred to dark from the low-light regime, the transpiration rate in the dark was 1.21 6 0.02 mmol m 22 s 21 compared with 0.73 6 0.06 mmol m 22 s 21 in the wild type. Similarly, when the plants were transferred from saturating light intensity, the transpiration rate in the dark of tmm1 was approximately 20% higher than that of plants with single stomata.To directly assess the impact of stomatal clustering on stomatal closing, we measured stomatal apertures after treatments with the well-established closing stimuli Ca 2+ and abscisic acid (ABA; Grabov and Blatt, 1998;Eisenach et al, 2012;Papanatsiou et al, 2015). Stomata of wild-type and PTMM1 plants closed to similar extents, whereas stomata of the tmm1 mutant Table I.…”

mentioning

confidence: 99%

“…We examined stomatal opening in stomatal clusters by treating epidermal peels with depolarizing buffer (60 mM KCl-MES, pH 6.1; Papanatsiou et al, 2015) under high light intensity for 2 h. Maximum opening of stomatal pores was measured by incorporating the geometry of individual guard cells as well as the whole stoma to calculate the area within the pore, as described previously (Doheny-Adams et al, 2012;Meckel et al, 2007). The wild-type and PTMM1 plants yielded stomatal apertures of 22.6 6 1.5 and 24.6 6 1.7 mm 2 , respectively (Fig.…”

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confidence: 99%

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Stomatal Spacing Safeguards Stomatal Dynamics by Facilitating Guard Cell Ion Transport Independent of the Epidermal Solute Reservoir

2016

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Stomata enable gaseous exchange between the interior of the leaf and the atmosphere through the stomatal pore. Control of the pore aperture depends on osmotic solute accumulation by, and its loss from the guard cells surrounding the pore. Stomata in most plants are separated by at least one epidermal cell, and this spacing is thought to enhance stomatal function, although there are several genera that exhibit stomata in clusters. We made use of Arabidopsis (Arabidopsis thaliana) stomatal patterning mutants to explore the impact of clustering on guard cell dynamics, gas exchange, and ion transport of guard cells. These studies showed that stomatal clustering in the Arabidopsis too many mouths (tmm1) mutant suppressed stomatal movements and affected CO 2 assimilation and transpiration differentially between dark and light conditions and were associated with alterations in K + channel gating. These changes were consistent with the impaired dynamics of tmm1 stomata and were accompanied by a reduced accumulation of K + ions in the guard cells. Our findings underline the significance of spacing for stomatal dynamics. While stomatal spacing may be important as a reservoir for K + and other ions to facilitate stomatal movements, the effects on channel gating, and by inference on K + accumulation, cannot be explained on the basis of a reduced number of epidermal cells facilitating ion supply to the guard cells.Stomata are pores found in the epidermis of most aerial parts of plants and are formed between a specialized pair of cells, the guard cells. Stomata facilitate the uptake of CO 2 at the expense of water vapor release via transpiration (Hetherington and Woodward, 2003). Hence, stomata play a crucial role in the physiology of plants. They permit gaseous exchange between the environment and the inside of the leaf for photosynthesis and, in turn, they influence the water use efficiency and growth of the plant. Mathematical models have suggested that historical changes in the freshwater resources can be attributed to stomatal transpiration, and it has been argued that the manipulation of stomata will be an important factor in ensuring water availability over the next 20 to 30 years (UNESCO World Water Development Report, 2015). Efforts to develop crops with higher water use efficiency through conventional breeding strategies have led to some successes, including the Drysdale wheat (Triticum aestivum) variety (Condon et al., 2002). It is likely that further advances will be possible as we gain insights into the physiology of stomata in situ.The regulation of gas exchange is achieved by controlling the stomatal pore. Stomata respond dynamically to environmental changes, including light quality and intensity, ambient CO 2 concentration, and humidity (Aphalo and Jarvis, 1991;Hetherington and Woodward, 2003;Shimazaki et al., 2007). Stomatal movements result from changes in guard cell volume and turgor and are driven by solute and water fluxes across the plasma membrane and tonoplast of guard cells. In particular, fluxes of ...

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Section: Stomatal Clustering Influences Ion Transport In Guard Cellssupporting

confidence: 67%

Section: Stomatal Clustering Influences Ion Transport In Guard Cellsmentioning

confidence: 94%

Section: Spacing Between Stomata Is Essential For Stomatal Closurementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Stomatal Spacing Safeguards Stomatal Dynamics by Facilitating Guard Cell Ion Transport Independent of the Epidermal Solute Reservoir

2016

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Cell Signalling Mechanisms in Plants

McAinsh

Taylor

2017

Encyclopedia of Life Sciences

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Plants are exposed to a wide range of environmental and developmental signals to which they must respond if they are to grow and reproduce. To allow this, plants have evolved complex mechanisms by which these different signals are perceived and transduced to bring about an appropriate physiological response. While these signalling pathways are highly diverse, they all possess two key properties: signal amplification and signal specificity. In addition, many of the components of plant cell signalling pathways are common to all eukaryotes. These include membrane receptors that recognise individual stimuli and numerous proteins, including kinase and phosphatase enzymes, and small molecules that transfer the signals from where they are perceived to their site of action within cells. However, the manner in which these components function in plants can often be different from how they function in other organisms. Key Concepts Plants monitor and respond to a diverse and continually changing stream of environmental and developmental signals. Chains of proteins and other signalling intermediates connect the perception of signals to the appropriate physiological response in cells. Signal transduction pathways share common properties. The plasma membrane is the main site of signal perception. Interaction between signalling pathways to form signalling networks within cells allows the integration of information from different signals. Second messengers and protein phosphorylation cascades allow amplification of signals. Cell signalling pathways can result in changes to cellular metabolism, function and movement, and altered gene expression and development.

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Hydrogen Sulfide in Guard Cell Signaling

Garcı́a-Mata

2019

Reactive Oxygen, Nitrogen and Sulfur Species in Plants

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Hydrogen Sulfide Regulates Inward-Rectifying K+ Channels in Conjunction with Stomatal Closure

Cited by 103 publications

References 60 publications

Stomatal Spacing Safeguards Stomatal Dynamics by Facilitating Guard Cell Ion Transport Independent of the Epidermal Solute Reservoir

Stomatal Spacing Safeguards Stomatal Dynamics by Facilitating Guard Cell Ion Transport Independent of the Epidermal Solute Reservoir

Cell Signalling Mechanisms in Plants

Hydrogen Sulfide in Guard Cell Signaling

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