Hydrogen suppresses oxidative stress by inhibiting the p38 MAPK signaling pathway in preeclampsia

Abstract

Background. Hypertensive disorders complicating pregnancy (HDCP) are one of the most serious medical disorders during pregnancy.Objectives. To investigate the effects of hydrogen on the mitogen-activated protein kinase (MAPK) signaling pathway in preeclampsia (PE). Materials and methods.The N(omega)-nitro-L-arginine methyl ester (L-NAME)-induced PE model with Sprague Dawley (SD) rats was employed. An inhibitor of MAPK signaling pathways (SB203580) was used as a p38 MAPK inhibitor. The SD rats were randomized into 5 groups: non-pregnant (NP); normal pregnancy (P); pregnancy + L-NAME (L); pregnancy + L-NAME + hydrogen-rich saline (LH); and pregnancy + L-NAME + hydrogen-rich saline + SB203580 (LHS). The pregnancies were terminated on day 22 of gestation, and the placentas and kidneys were microscopically inspected. Tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and malondialdehyde (MDA) levels were assessed. The mean systolic blood pressure (SBP) and level of proteinuria were recorded. The p38 MAPK mRNA expression and p-p38 MAPK protein levels were measured using real-time polymerase chain reaction (RT-PCR) and western blot, respectively.Results. It was found that hydrogen-rich saline (LH group) decreased placental MDA, proteinuria, TNF-α, and IL-1β levels in the placental tissues compared with the L group (all p < 0.05). Additionally, hydrogen-rich saline (LH group) treatment significantly decreased the p38 MAPK mRNA expression and p-p38 MAPK protein levels compared with the L group (p < 0.05). The p38 MAPK inhibitor SB203580 (LHS group) further decreased the p38 MAPK mRNA expression and p-p38 MAPK protein levels compared with the LH group (p < 0.05). Conclusions.Hydrogen can decrease the reactive oxygen species (ROS) content and inhibit the MAPK pathway. The protective effect of hydrogen may be associated with the inhibition of the p38 MAPK signaling pathway.

Quercetin attenuates lipopolysaccharide-induced hepatic inflammation by modulating autophagy and necroptosis

“…It might be associated with the hydrogen ion level of chicken embryos. Previous study revealed that the hydrogen ion level cumulated and increased with hatch age for chicken embryos ( Murray and Assistance of Yetta, 1926 ), and another study indicated that the hydrogen-rich saline treatment significantly decreased the p38 MAPK mRNA expression and p-p38 MAPK protein level in the placentas of pregnant rats ( Guo et al, 2023 ). Therefore, the hydrogen ion level increased when the PBS injected into the chick embryos, it significantly decreased the mRNA expression of p38.…”

The suppression of MAPK/NOX/MMP signaling prompts renoprotection conferred by prenatal naproxen in weaning preeclamptic rats

“…It is widely recognized that oxidative and fibrotic bursts are key constituents that fine-tune and transduce the inflammatory response prompted by MAPK signaling. Recent data from studies on cardiorenal injuries suggests that the activation of the pro-oxidant NADPH oxidase following MAPKs phosphorylation results in the upregulation of pro-fibrotic transforming growth factor β1 and extracellular degradation signals regulated by MMPs 49 – 51 . The latter molecules are necessary for provoking tissue transition from the inflammatory phase to the fibrotic remodeling phase 52 , 53 .…”