In this study, the hydrogen embrittlement (HE) of 316L stainless steel produced by laser powder bed fusion (L-PBF) was investigated by means of hydrogen trapping. The susceptibility of the material to HE is strongly connected to the interaction of hydrogen atoms with volumetric defects in the material. Trapping hydrogen in those defects affects its availability to critical locations where a hydrogen-induced crack can nucleate. Therefore, it is important to study the characteristics of hydrogen traps to better understand the behavior of the material in the hydrogen environment. The hydrogen was introduced into the material via electrochemical charging, and its interactions with various trapping sites were studied through thermal desorption spectroscopy (TDS). The obtained results were compared to conventionally produced 316L stainless steel, and the correlation between microstructure, characteristics of hydrogen traps, and susceptibility to HE is discussed.