Hydrogenation of biomass pyrolysis oil using enhanced reduction catalytic capacity cellular by expressing excavated carbonyl reductase

Bu, Chongyang; Qiao, Hui; Zou, Lihua; Tao, Yuanming; Fu, Jiaming; Liu, Chao; Zheng, Zhaojuan; Ouyang, Jia

doi:10.1016/j.fuel.2022.126108

Cited by 7 publications

(2 citation statements)

References 56 publications

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“…In addition to IPA, compared with the blank group, the other three organic-aqueous systems had certain promoting effects, and the ethyl acetate (EA) aqueous system could give the highest catalytic efficiency. Shah constructed and applied a biphasic bioelectrocatalytic system [72]. To support the AdhS-catalyzed the bioconversion of COBE (100 mM) to (R)-CHBE for 10 h, the product yield reached only 85%.…”

Section: Enhancement In Cobe Bioreduction Through Supply Of Organic S...mentioning

confidence: 99%

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Yang,

Xu,

Jiang

et al. 2023

Processes

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Objective: Optically active (R)-ethyl 4-chloro-3-hydroxybutyrate ((R)-CHBE) is a useful chiral building block for the synthesis of pharmaceuticals. Recently, there has been great interest in the synthesis of (R)-CHBE via the highly stereoselective bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE) under mild conditions. Methods: A highly efficient bioreduction process for transforming COBE into (R)-CHBE was developed in a biocompatible organic solvent–deep eutectic solvent–water reaction medium. Results: Recombinant Escherichia coli containing carbonyl reductase (CgCR) and glucose dehydrogenase (GDH) was successfully constructed and characterized. In addition, the feasibility of the asymmetric bioreduction of COBE to (R)-CHBE was verified in an organic solvent–deep eutectic solvent–water (ethyl acetate-betaine/lactic acid-water) system. At pH 7.0 and 30 °C, the kinetic constants Km and kcat of COBE were 20.9 mM and 56.1 s−1, respectively. A high (R)-CHBE yield (≥90%) was achieved by catalyzing COBE (1000 mM) in 12 h with E. coli CgCR cells in the presence of Ni2+ (7 mM) and glucose (3.5 mM glucose/mM COBE) in an ethyl acetate-betaine/lactic acid-H2O (50/7/43, v/v/v) system. The effects of organic solvents and DESs on whole-cell permeability were analyzed. Conclusions: An efficient bioreduction system was constructed for biologically transforming COBE to (R)-CHBE via whole-cell biocatalysis, and the established bioprocess has potential application in future.

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Section: Enhancement In Cobe Bioreduction Through Supply Of Organic S...mentioning

confidence: 99%

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Yang,

Xu,

Jiang

et al. 2023

Processes

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“…Notably, prevailing adhesives employed in wood panel fabrication are predominantly petroleum based, encompassing urea-formaldehyde (UF) resins, melamine and phenol-formaldehyde (PF) resins, as well as epoxy (EP) and polyurethane (PU) resins, etc. The finite nature of petroleum resources and environmental repercussions arising from their extraction necessitate a shift towards leveraging abundant but underutilized renewable biomass [9][10][11][12][13][14][15] resources. The application of biomass resources in wood adhesives, in addition to bolstering environmental preservation, aligns with sustainable resource management imperatives.…”

Section: Introductionmentioning

confidence: 99%