In order to collect and unify data about all geothermal resources in Serbia, a database is formed. The database allows us to perceive the geothermal resources of Serbia and their potential for utilization. Based on the data available in the geothermal database, the estimated temperatures of reservoirs, heat power, and geothermal energy utilization were calculated. The database contains 293 objects (springs, boreholes) registered at 160 locations with groundwater temperature in the range between 20°C and 111°C. The maximum expected temperature of the reservoir is 146°C (according to the SiO2 geothermometer). Some thermal water is cooled while mixed with cold, shallow water. Geothermal resources are mostly used for balneology and recreation, and less for heating, water supply, bottling, fish and animal farms, agriculture, and technical water. 26% of all geothermal resources is used by the local population or has not been used at all. The annual utilization of geothermal energy for direct heat is 1507 TJ/yr, and the estimated capacity of geothermal energy in Serbia is 111 MWt. The results of analytical work were presented in the form of maps with a geological and hydrogeological background. Thermal waters are mostly registrated in the area of Tertiary magmatism. The three geothermal potential areas are identified: Pannonian basin-Vojvodina Province, Mačva-Srem and area from Jošanička Banja to Vranjska Banja (southern Serbia). Based on chemical analyses, four hydrochemical facies are distinguished. Thermal water mainly belongs to NaHCO3 or CaMgHCO3 hydrochemical facies, usually depending on the primary aquifer: karst, karst-fissured, intergranular or fissured.