Abstract. Measurements of the stable isotopes oxygen-18 ( 18 O) and deuterium ( 2 H) were carried out in two meso-scale catchments, Chemoga (358 km 2 ) and Jedeb (296 km 2 ) south of Lake Tana, Abay/Upper Blue Nile basin, Ethiopia. The region is of paramount importance for the water resources in the Nile basin, as more than 70 % of total Nile water flow originates from the Ethiopian highlands. Stable isotope compositions in precipitation, spring water and streamflow were analysed (i) to characterise the spatial and temporal variations of water fluxes; (ii) to estimate the mean residence time of water using a sine wave regression approach; and (iii) to identify runoff components using classical two-component hydrograph separations on a seasonal timescale.The results show that the isotopic composition of precipitation exhibits marked seasonal variations, which suggests different sources of moisture generation for the rainfall in the study area. The Atlantic-Indian Ocean, Congo basin, Upper White Nile and the Sudd swamps are the potential moisture source areas during the main rainy (summer) season, while the Indian-Arabian and Mediterranean Sea moisture source areas during little rain (spring) and dry (winter) seasons. The spatial variation in the isotopic composition is influenced by the amount effect as depicted by moderate coefficients of determination on a monthly timescale (R 2 varies from 0.38 to 0.68) and weak regression coefficients (R 2 varies from 0.18 to 0.58) for the altitude and temperature effects. A mean altitude effect accounting for −0.12 ‰/100 m for 18 O and −0.58 ‰/100 m for 2 H was discernible in precipitation isotope composition.Results from the hydrograph separation on a seasonal timescale indicate the dominance of event water, with an average of 71 and 64 % of the total runoff during the wet season in the Chemoga and Jedeb catchments, respectively.Moreover, the stable isotope compositions of streamflow samples were damped compared to the input function of precipitation for both catchments. This damping was used to estimate mean residence times of stream water of 4.1 and 6.0 months at the Chemoga and Jedeb catchment outlets, respectively. Short mean residence times and high fractions of event water components recommend catchment management measures aiming at reduction of overland flow/soil erosion and increasing of soil water retention and recharge to enable sustainable development in these agriculturally dominated catchments.