The partition of carotenoids and vitamin A in the colostrum of nine cows and six goats and in the milk of three cows and two goats was determined using chromatographic and spectrophotometric methods. The following were the main results:1. The content of β-carotene and vitamin A in the colostrum decreased rapidly during the first nine milkings in both the cow and the goat. The rates of decline were logarithmic. Logarithmic regressions also showed that the decreases in β-carotene were more rapid than those of vitamin A.2. The heifers secreted more vitamin A in their colostral fat than did cows on the same ration. The vitamin A content of the colostral fat of goats was greater during the first lactation than in later lactations. There was no difference in the rates of decrease of the vitamin A content of heifer and cow colostrum, but the difference between the rates of decreases of colostral vitamin A of first and later lactation goats was significant.3. The colostral fat of cows at pasture contained more vitamin A than that of cows on winter rations. There was no difference between the logarithmic rates of decrease in the two groups during the first nine milkings. The colostral fat of four goats receiving winter rations was richer in vitamin A than that of three cows treated similarly, and this superiority of the goat over the cow persisted throughout lactation.4. There was no difference between the carotene content of the colostral fat of the first and later lactation cows. The yield of fat and β-carotene of the heifers was smaller than that of the cows. Cows at pasture secreted more β-carotene in their colostrum than the cows on winter rations. The presence of β-carotene was demonstrated in goat colostrum, although mature goat milk was free from any measurable quantity of carotenoids.