Hydrolysis of casein: a differential aid for the indentification ofSerratia marcescens

Salisbury, William A.; Likos, Joseph J.

doi:10.1136/jcp.25.12.1083

Cited by 11 publications

(5 citation statements)

References 11 publications

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“…The Gram-negative, rod-shaped cells are facultatively anaerobic and motile. S. marcescens is a saprotroph that produces a number of extracellular enzymes including DNAase, lipase and gelatinase [26]; it efficiently hydrolyses chitin and casein [5,27] and is also an opportunistic pathogen (e.g. causes hospital-acquired infections in immunocompromised patients) [23] Some strains are non-pigmented (including the majority of clinical isolates), but those of environmental origin are typically pigmented [29,30].…”

Section: Hahella Chejuensismentioning

confidence: 99%

Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens

Suryawanshi

Patil

Borase

et al. 2014

Intern J of Cosmetic Sci

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The bacterial pigments prodigiosin and violacein exhibited antioxidant and antimicrobial activities and were able to increase the SPF of commercial sunscreens as well as the extracts of the two plant species tested. These pigments have potential as ingredients for a new product range of and, indeed, represent a new paradigm for sunscreens that utilize substances of biological origin. We discussed the biotechnological potential of these bacterial metabolites for use in commercial sunscreens, and the need for studies of mammalian cells to determine safety.

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Section: Hahella Chejuensismentioning

confidence: 99%

Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens

Suryawanshi

Patil

Borase

et al. 2014

Intern J of Cosmetic Sci

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“…The strain has more secretory protein in the cell-free supernatant. Enzymatic activity was observed using casein as a substrate (Salisbury and Likos, 1972), where S. marcescens VS56 has 12.31 units/mL of active enzyme. When compared with the study of Koul et al, 2021 the isolates S. marcescens MES-4 and S. marcescens MRS-11 from mulberry soil had protease activity of 95 units/mL and 156 units/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of serratiopeptidase-producing bacteria from different sources

Nair,

Subathra Devi

2024

Front. Microbiol.

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Anti-inflammatory enzymes have wide applications in the pharmaceutical industry. The objective of this study was to find new and efficient strains for the commercial production of serratiopeptidase enzyme. Vast number of samples were processed for the isolation of potent strains. The experimental treatment includes processing of twenty soil samples, silkworm gut, and sugarcane stem. The total protein and protease activity was estimated by Lowry’s method and casein hydrolysis. The HRBC stabilization assay was performed for finding the anti-inflammatory potential of all strains. The serratiopeptidase production was confirmed by HPLC with the standard. Molecular characterization of selected potent strains was done by 16S rDNA and confirmed the taxonomy. The one step rapid purification of serratiopeptidase was performed by Ultra three phase partitioning method. The clot lysis potential of the Serratia marcescens VS56 was observed by modified Holmstorm method. The results of the study revealed that among the 60 strains, 12 strains were protease-positive on skim milk agar plates and showed significant protease activity. All 12 strains were screened for serratiopeptidase using high-performance liquid chromatography (HPLC) and VS56, VS10, VS12 and VS18 showed a similar retention time (4.66 ± 0.10 min) with standard. The selected potent strain, Serratia marcescens VS56 showed a proteolytic activity of 21.30 units/mL and produced a total protein of 102 mg/mL. The HRBC suspension results also showed a percentage of 94.6 ± 1.00 protection, which was compared to the standard diclofenac. The clot lysis potential of Serratia marcescens VS56 was 53% in 4 h. Furthermore, the molecular weight of the protein was identified to confirm the presence of serratiopeptidase. The study hence contributed successfully to isolating, screening, and identifying a potent producer for serratiopeptidase from an environmental source. This inherent advantage of the strain will undoubtedly contribute much to the coco comm commercial production of serratiopeptidase in the near future.

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“…Detection of proteolytic activity was examined by a casein hydrolysis test [ 22 ]. One loopful of 10 T. phagedenis strains isolated from PDD lesions was inoculated onto a modified skim milk agar (mSMA) plate, containing GAM agar (Nissui Pharmaceutical) supplemented with 0.8% Brain Heart Infusion broth (Nissui Pharmaceutical), 0.8% Brucella broth (Becton Dickinson and Co.), 10% skim milk (Becton Dickinson and Co.) and 10% heat inactivated fetal bovine serum (GIBCO, Thermo Fisher Scientific), and then incubated at 37°C for 7–10 days under anaerobic conditions as described above.…”

Section: Methodsmentioning

confidence: 99%

Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro

Khemgaew

Omachi

Takesada

et al. 2021

The Journal of Veterinary Medical Science

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Papillomatous digital dermatitis (PDD) is a polymicrobial infection causing lameness in dairy cattle. Culture-independent analysis has shown that Treponema phagedenis is present consistently and predominantly in the lesions. However, the pathogenesis of PDD, especially the tissue penetration pathway, has not been examined. In the present study, we investigated whether T. phagedenis strains isolated from PDD produce proteolytic enzyme(s) for disruption of the epithelial cell barrier and have the ability to translocate in polarized normal human epidermal keratinocytes (NHEK) in vitro. Ten strains of T. phagedenis isolated from lesions did not show proteolytic activity on modified skim milk agar, although a human strain of T. denticola used as a control showed such activity. The integrity of tight junctions was monitored by measurement of transepithelial electrical resistance (TER). The TER values after inoculation of the T. phagedenis strains examined did not change during the experimental period; however, apical to basolateral translocation of T. phagedenis was confirmed after 24 hr by microscopy and Treponema-specific PCR. We further confirmed that translocation of T. phagedenis was accelerated by co-inoculation with live T. denticola, but not with heat-killed organisms. Furthermore, tight junction ZO-1 protein was not lost intensity after inoculation with T. phagedenis and the organism was observed in NHEK cells using a florescence microscope. These results suggest that T. phagedenis strains may translocate via a transcellular route in vitro and that the invasion is accelerated by other bacteria, such as T. denticola, producing proteolytic activity.

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Hydrolysis of casein: a differential aid for the indentification ofSerratia marcescens

Cited by 11 publications

References 11 publications

Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens

Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens

Bioprospecting of serratiopeptidase-producing bacteria from different sources

Transcellular penetration of <i>Treponema phagedenis</i> isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes <i>in vitro</i>

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