Hydrolysis of casein from different sources by immobilized trypsin on biochar: Effect of immobilization method

Júnior, Evaldo Cardozo de Souza; Santos, Mateus Pereira Flores; Sampaio, Vanessa Santos; Ferrão, Sibelli Passini Barbosa; Fontan, Rafael da Costa Ilhéu; Bonomo, Renata Cristina Ferreira; Veloso, Cristiane Martins

doi:10.1016/j.jchromb.2020.122124

Cited by 16 publications

(11 citation statements)

References 37 publications

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“…The C-terminal ends of all casein sequences are highly unstructured compared to their N-terminal ends, which are well-structured. The phosphorylated sites of the casein were poorly hydrolyzed, which is in agreement with previous studies ( 42 – 44 ).…”

Section: Discussionsupporting

confidence: 93%

Phylogenetic comparative analysis: Chemical and biological features of caseins (alpha-S-1, alpha-S-2, beta- and kappa-) in domestic dairy animals

et al. 2022

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Caseins determine the physicochemical, physiological, and biological characteristics of milk. Four caseins—alpha-S-1, alpha-S-2, beta, and kappa—were analyzed phylogenetically and in silico and characterized regarding chemical, antimicrobial, and antioxidant features in five dairy animals: Arabian camels, sheep, goats, cattle, and water buffalos. The sequence of full-length amino acids of the four caseins for the five species was retracted from the NCBI GenBank database. Multiple sequence alignment is used to examine further the candidate sequences for phylogenetic analysis using Clustal X and NJ-Plot tools. The results revealed that sheep and goats possess strong similarities (98.06%) because of their common ancestor. The same was observed with cattle and water buffalos (96.25%). The Arabian camel was located in a single subclade due to low similarity in casein residues and compositions with other dairy animals. Protein modeling showed that alpha-S1- and alpha-S2-caseins possess the highest number of phosphoserine residues. The in silico computed chemical properties showed that β-casein recorded highest hydrophobicity index and lowest basic amino acid content, while α-S2-casein showed the opposite. The computed biological parameters revealed that α-S2-casein presented the highest bactericidal stretches. Only Arabian camel β-casein and k-casein showed one bactericidal stretches. The analysis also revealed that β-casein, particularly in Arabian camels, possesses the highest antioxidant activity index. These results support the importance of the bioinformatics resources to determine milk casein micelles' chemical and biological activities.

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Section: Discussionsupporting

confidence: 93%

Phylogenetic comparative analysis: Chemical and biological features of caseins (alpha-S-1, alpha-S-2, beta- and kappa-) in domestic dairy animals

et al. 2022

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“…They work together with chymotrypsin and carboxypeptidase in the metabolic processing of protein hydrolysis into easily absorbed essential peptides and amino acids. These amino acids have several functions in the body, such as muscle growth and hormone production [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The selection of the immobilization method should be based on various parameters such as the process efficiency, the costs of the immobilization procedure, the toxicity of the immobilization reagents, and the final properties required in the derivative [ 13 ]. In general, adsorption and covalent bonding are the most used techniques for enzyme immobilization [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Proteases: Importance, Immobilization Protocols, Potential of Activated Carbon as Support, and the Importance of Modifying Supports for Immobilization

Santos,

de Souza Junior,

Villadóniga

et al. 2024

BioTech

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“…Activated carbon is a porous carbonaceous material, having high Brunauer–Elmett–Teller (BET) surface area and thermal, chemical and mechanical resistance 18,30,31 . Basically, this material is obtained by processes that involve the carbonization of the carbon precursor material and its activation 18,32,33 .…”

Section: Introductionmentioning

confidence: 99%

“…29 Activated carbon is a porous carbonaceous material, having high Brunauer-Elmett-Teller (BET) surface area and thermal, chemical and mechanical resistance. 18,30,31 Basically, this material is obtained by processes that involve the carbonization of the carbon precursor material and its activation. 18,32,33 The chemical and textural characteristics of the material, combined with the reduced cost of production when synthesized from lignocellulosic biomass (agro-industrial residues), make this an advantageous support option, when compared to other matrices, to promote the binding of metallic particles, since the support produced is promising for immobilization of lipases, providing a high affinity for proteins and facilitating the separation and reuse of the enzyme, through the external magnetic field that can be formed.…”

Section: Introductionmentioning

confidence: 99%

Incorporation of metallic particles in activated carbon used in lipase immobilization for production of isoamyl acetate

Oliveira

Santos

Brito

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND: Biotechnology has revolutionized the use of enzymes for application in industrial processes. The present work evaluated the effect of functionalizing activated carbon with metallic nanoparticles obtained from corn cob residue on lipase immobilization. The aim was to improve the enzymatic activity of the immobilized enzyme and subsequent application of derivatives in the synthesis of isoamyl acetate ester (banana flavor). RESULTS:The activated carbon (CCAC) had a mesoporous structure and specific surface area of 1135 m 2 g −1 , characteristic of supports from precursor material with a high cellulose content, which was reduced in metalized carbon (CCACM) due to the insertion of metallic particles (760 m 2 g −1 ). CCACM showed a slightly higher lipase absorptive capacity than CCAC (280.72 ± 0.27 mg g −1 and 276.46 ± 0.03 mg g −1 , respectively). Furthermore, for derivatives the optimal reaction medium conditions were pH 7.0 and 30 °C, confirming the advantage associated with lipase immobilization, obtaining activity values of 84.0 ± 2.12 U, 72.3 ± 1.7 U and 31.4 ± 1.9 U, for lipase immobilized on CCACM, CCAC and native form, respectively. The derivatives showed potential application in the synthesis of isoamyl acetate ester, with conversions above 90% and stability during five cycles of use. CONCLUSION:The activated carbon metallization process showed an increase in lipase immobilization efficiency and proved to be a promising support, since there was an increase in its enzymatic activity and the derivatives obtained showed potential application in aroma synthesis, reaching conversion above 90%.

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Hydrolysis of casein from different sources by immobilized trypsin on biochar: Effect of immobilization method

Cited by 16 publications

References 37 publications

Phylogenetic comparative analysis: Chemical and biological features of caseins (alpha-S-1, alpha-S-2, beta- and kappa-) in domestic dairy animals

Phylogenetic comparative analysis: Chemical and biological features of caseins (alpha-S-1, alpha-S-2, beta- and kappa-) in domestic dairy animals

Proteases: Importance, Immobilization Protocols, Potential of Activated Carbon as Support, and the Importance of Modifying Supports for Immobilization

Incorporation of metallic particles in activated carbon used in lipase immobilization for production of isoamyl acetate

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