Hydrolysis of Cefotaxime by a beta-lactamase from Bacteroides fragilis

Péchère, Jean-Claude; Guay, R.; Dubois, Jacques; Letarte, Robert

doi:10.1128/aac.17.6.1001

Cited by 19 publications

(9 citation statements)

References 13 publications

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“…Since 91% of the B. fragilis isolates elaborated a cephalosporinase, it is not surprising that 7P-methoxyiminoacetamido cephalosporins such as cefotaxime, ceftizoxime, and cefmenoxime were not particularly effective against B. fragilis. Our figures on the correlation of the antibacterial activity and the stability of cefoxitin and cefotaxime to B. fragilis ,B-lactamase are consistent with those of Pechere et al (15), who reported on the comparative hydrolysis of cefoxitin and cefotaxime by B. fragilis MULB-1008 ,-lactamase. Thus, the antibacterial activities of both the 7p-methoxy and the 7,3-methoxyiminoacetamido cephalosporins tested against B. fragilis correlate well with their stability to P-lactamase.…”

Section: Discussionsupporting

confidence: 81%

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis

Kesado¹,

Watanabe²,

Asahi³

et al. 1984

Antimicrob Agents Chemother

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The in vitro antibacterial activities of the newly developed 7a-methoxy cephalosporins and 7I-methoxyiminoacetamido cephalosporins against 67 clinical isolates of Bacteroides fragilis and their resistance to the hydrolytic action of a ,-lactamase produced by B. fragilis were simultaneously compared. The minimal inhibitory concentrations that inhibited 90% of the 7a-methoxy cephalosporins, cefoxitin, cefmetazole, moxalactam, and cefotetan, against the isolates were 4, 8, 8, and 16 jig/ml, respectively, and these antibiotics were entirely resistant to hydrolysis by ,-lactamases (0.10 ,umol/h per mg of protein) of the isolates. By contrast, 7p-methoxyiminoacetamido cephalosporins represented by cefotaxime, ceftizoxime, and cefmenoxime were not effective, as indicated by the minimal inhibitory concentrations that inhibited 90%, 64, 32, and 128 ,ug/ml, respectively. Their antibacterial activities clearly corresponded to their resistance to the hydrolytic action of the P-lactamase: namely, the correlation coefficients in regression curves of cefotaxime, ceftizoxime, and cefmenoxime, which were expressed by the antibacterial activity (x axis) and the ,-lactamase activity (y axis) were 0.098, 0.034, and 0.163, respectively.In recent years, anaerobic bacteria, especially Bacteroides fragilis, have been isolated from clinical specimens with increasing frequency (6,8). Several investigators have reported that B. fragilis strains produce a ,B-lactamase which has cephalosporinase activity and that it plays a significant role in the resistance of these organisms to B-lactam compounds (2,11,13,17,22). On the other hand, several newlactam compounds have been developed in recent years for the treatment of infections caused by resistant organisms which produce a ,-lactamase (1,7,9,20). Prominent among such compounds are cefoxitin, cefmetazole, moxalactam, and cefotetan, which are grouped as 7a-methoxy cephalosporins, and cefotaxime, ceftizoxime, and cefmenoxime, which are included in the 7p-methoxyiminoacetamido cephalosporin group. Both groups are currently in clinical use. The present study was undertaken to compare the in vitro antibacterial activities of 7a-methoxy cephalosporins and 7,3-methoxyiminoacetamido cephalosporins against 67 clinical isolates of B. fragilis and to investigate whether there exists a correlation between their antibacterial activity and the resistance to the hydrolytic action of ,B-lactamases produced by B. fragilis. MATERIALS AND METHODSOrganisms. All of the 67 B. fragilis isolates tested were obtained from clinical specimens and were identified at the Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Japan, according to the criteria of the Virginia Polytechnic Institute Anaerobe Laboratory Manual (21). Organisms were maintained at room temperature in tubes containing GAM semisolid medium (1% peptone, 0.3% soya peptone, 1% proteose peptone, 1.35% serum digest powder, 0.5% yeast extract, 0.22% beef extract powder, 0.12% liver extract powder, 0.3% glucose, 0.25% potassium dehydro...

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Section: Discussionsupporting

confidence: 81%

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis

Kesado¹,

Watanabe²,

Asahi³

et al. 1984

Antimicrob Agents Chemother

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“…For all assays, controls were performed by omitting from the reaction mixture the specifi c substrate. Kinetic parameters were estimated from a least-squares fi t of Lineweaver-Burk plots (Pechère et al, 1980).…”

Section: Methodsmentioning

confidence: 99%

“…PFGE analysis PFGE was performed to determine whether clonal spreading was responsible for the dissemination of CTX-M-3-derivatives or not based on the criteria previously described by Tenover et al (Pechère et al, 1980). In this study, the two CTX-M-3-derivative strains isolated from different hospitals belonged to the same clones that exhibited closely related isolates (Fig.…”

Section: Kinetic Parametersmentioning

confidence: 99%

Phenotypic and molecular characterization of a novel β-lactamase carried by Klebsiella pneumoniae, CTX-M-72, derived from CTX-M-3

Cheng

Wang

Chen

et al. 2009

J. Gen. Appl. Microbiol.

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“…A number of studies have correlated enzyme production with resistance to a variety of antimicrobial agents, including cephalosporins (5,7,19,21). However, investigators have also reported the resistance of 3-lactamase-negative isolates of Bacteroides spp.…”

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confidence: 99%

Activity of cefoperazone and two beta-lactamase inhibitors, sulbactam and clavulanic acid, against Bacteroides spp. correlated with beta-lactamase production

Crosby¹,

Gump²

1982

Antimicrob Agents Chemother

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A total of 102 isolates of Bacteroides spp. were studied for P-lactamase production and susceptibility to cefoperazone alone or in combination with either of the P-lactamase inhibitors sulbactam and clavulanic acid. The geometric mean minimal inhibitory concentration of cefoperazone alone was 31.5 ,ug/ml and when combined with 10 ,g of sulbactam per ml or 2 ,ug of clavulanic acid per ml was reduced to 5.4 and 9.2 ,ug/ml, respectively. When bacterial suspensions were tested for P-lactamase production with nitrocefin, 91 (89.2%) of these isolates produced the enzyme. The geometric mean minimal inhibitory concentrations of cefoperazone rose only slightly for isolates with low or intermediate enzyme activity but rose significantly for those with high activity. The addition of EDTA to cefoperazone significantly more frequently enhanced the activity of cefoperazone against P-lactamase-negative as opposed to ,-lactamase-positive isolates.Furthermore, EDTA resulted in synergistic activity of the cefoperazone-sulbactam combination on P-lactamase-positive isolates for which the combination had previously not shown a synergistic effect. This study demonstrates the relationship between P-lactamase production and the resistance of Bacteroides spp. to cefoperazone and shows that inhibition of these enzymes can reverse this resistance.

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Hydrolysis of Cefotaxime by a beta-lactamase from Bacteroides fragilis

Cited by 19 publications

References 13 publications

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis

Phenotypic and molecular characterization of a novel β-lactamase carried by Klebsiella pneumoniae, CTX-M-72, derived from CTX-M-3

Activity of cefoperazone and two beta-lactamase inhibitors, sulbactam and clavulanic acid, against Bacteroides spp. correlated with beta-lactamase production

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