Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks

Ahmed, Ibrahim Nasser; Yang, Xiuli; Dubale, Amare Aregahegn; Li, Ruo-Fei; Ma, Yiming; Wang, Luming; Hou, Guihua; Guan, Rongfeng; Xie, Mingjun

doi:10.1016/j.biortech.2018.09.077

Cited by 97 publications

(28 citation statements)

References 37 publications

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“…The samples were degassed at 50 °C for 3 h prior to N 2 adsorption. The specific surface area of the sample was calculated by using the multiple-point Brunauer–Emmett–Teller (BET) method in the relative pressure range (P/Po) of 0.05–0.3 [20].…”

Section: Methods/experimentalmentioning

confidence: 99%

Copper doped zeolite composite for antimicrobial activity and heavy metal removal from waste water

et al. 2019

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Background The existence of heavy metals and coliform bacteria contaminants in aquatic system of Akaki river basin, a sub city of Addis Ababa, Ethiopia has become a public concern as human population increases and land development continues. Hence, it is the right time to design treatment technologies that can handle multiple pollutants. Results In this study, we prepared a synthetic zeolites and copper doped zeolite composite adsorbents as cost effective and simple approach to simultaneously remove heavy metals and total coliforms from wastewater of Akaki river. The synthesized copper–zeolite X composite was obtained by ion exchange method of copper ions into zeolites frameworks. Iodine test, XRD, FTIR and autosorb IQ automated gas sorption analyzer were used to characterize the adsorbents. The mean concentrations of Cd, Cr, and Pb in untreated sample were 0.795, 0.654 and 0.7025 mg/L respectively. These concentrations decreased to Cd (0.005 mg/L), Cr (0.052 mg/L) and Pb (bellow detection limit, BDL) for sample treated with bare zeolite X while a further decrease in concentration of Cd (0.005 mg/L), Cr (BDL) and Pb (BDL) was observed for the sample treated with copper–zeolite composite. Zeolite X and copper-modified zeolite X showed complete elimination of total coliforms after 90 and 50 min contact time respectively. Conclusion The results obtained in this study showed high antimicrobial disinfection and heavy metal removal efficiencies of the synthesized adsorbents. Furthermore, these sorbents are efficient in significantly reducing physical parameters such as electrical conductivity, turbidity, BOD and COD.

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Section: Methods/experimentalmentioning

confidence: 99%

Copper doped zeolite composite for antimicrobial activity and heavy metal removal from waste water

et al. 2019

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“…The maximum activity gained was 85% at 55 °C while utilized at 80 °C and the residual activities were 72% after ten cycles and 65% after 30 days storage. The development of cellulase-MOF composite with ultrahigh operations and durability for research revealed the auspicious future by this study [132].…”

Section: Cellulases Immobilization On Metal Organic Frameworkmentioning

confidence: 88%

“…The development of novel cellulase immobilized magnetic organic framework composite system with increased reusability and stability for cellulose hydrolysis was performed by Ahmed et al [132] using physical absorption method. The extra anchoring sites of NH2 groups showed higher protein loading by NH2-functionalized metal organic framework as compared to the precursor UiO-66.…”

Section: Cellulases Immobilization On Metal Organic Frameworkmentioning

confidence: 99%

Cellulose-deconstruction potential of nano-biocatalytic systems: A strategic drive from designing to sustainable applications of immobilized cellulases

Qamar

Bilal

et al. 2021

International Journal of Biological Macromolecules

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“…Several techniques have been developed for the confinement of cellulases including adsorption [29], entrapment [30], encapsulation [31], and chemical complex formation [32]. Moreover, a wide range of materials have been proposed as supports for cellulase immobilisation like porous silica [33][34][35], paramagnetic particles (Fe 2 O 3 ) [36,37] or cobalt ferrite nanoparticles [38]), and organic polymeric materials [39].…”

Section: Introductionmentioning

confidence: 99%

Facile Cellulase Immobilisation on Bioinspired Silica

et al. 2022

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Cellulases are enzymes with great potential for converting biomass to biofuels for sustainable energy. However, their commercial use is limited by their costs and low reusability. Therefore, the scientific and industrial sectors are focusing on finding better strategies to reuse enzymes and improve their performance. In this work, cellulase from Aspergillus niger was immobilised through in situ entrapment and adsorption on bio-inspired silica (BIS) supports. To the best of our knowledge, this green effect strategy has never been applied for cellulase into BIS. In situ entrapment was performed during support synthesis, applying a one-pot approach at mild conditions (room temperature, pH 7, and water solvent), while adsorption was performed after support formation. The loading efficiency was investigated on different immobilisation systems by Bradford assay and FTIR. Bovine serum albumin (BSA) was chosen as a control to optimize cellulase loading. The residual activity of cellulase was analysed by the dinitro salicylic acid (DNS) method. Activity of 90% was observed for the entrapped enzyme, while activity of ~55% was observed for the adsorbed enzyme. Moreover, the supported enzyme systems were recycled five times to evaluate their reuse potential. The thermal and pH stability tests suggested that both entrapment and adsorption strategies can increase enzyme activity. The results highlight that the entrapment in BIS is a potentially useful strategy to easily immobilise enzymes, while preserving their stability and recycle potential.

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Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks

Cited by 97 publications

References 37 publications

Copper doped zeolite composite for antimicrobial activity and heavy metal removal from waste water

Copper doped zeolite composite for antimicrobial activity and heavy metal removal from waste water

Cellulose-deconstruction potential of nano-biocatalytic systems: A strategic drive from designing to sustainable applications of immobilized cellulases

Facile Cellulase Immobilisation on Bioinspired Silica

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