Hydrolysis of fourteen native dextrans by arthrobacter isomaltodextranase and correlation with dextran structure

Sawai, Takeshi; Tohyama, Taiji; Natsume, T.

doi:10.1016/s0008-6215(00)83252-8

Cited by 33 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Native dextrans were hydrolysed enzymatically with various endo-and exodextranases to elucidate more accurately structural differences and their degree of branching [40][41][42]. Kobayashi and Matsuda [43] demonstrated that the glucoamylase from Rhizopus niveus can be used to remove Dglucose residues from the non-reducing ends of dextran, its action being stopped at the branch points, thus giving information on the side chain length.…”

Section: Homopolysaccharidesmentioning

confidence: 99%

Exocellular polysaccharides produced by lactic acid bacteria

Cerning¹

1990

FEMS Microbiology Letters

429

233

View full text Add to dashboard Cite

The production of homopolysaccharides (dextrans, mutans) and heteropolysaccharides by lactic acid bacteria, their chemical composition, their structure and their synthesis are outlined. Mutans streptococci, which include Streptococcus mutans and S. sobrinus produce soluble and insoluble α‐glucans. The latter may contain as much as 90%α‐1–3 linkages and possess a marked ability to promote adherence to the smooth tooth surface causing dental plaque. Dextrans produced by Leuconostoc mesenteroides are high molecular weight α‐glucans having 1–6, 1–4 and 1–3 linkages, varying from slightly to highly branched; 1–6 linkages are predominant. Emphasis is put on exopolysaccharide producing thermophilic and mesophilic lactic acid bacteria, which are important in the dairy industry. The produced polymers play a key role in the rheological behaviour and the texture of fermented milks. One of the main problems in this field is the transitory nature of the thickening trait. This instability is not yet completely understood. Controversial results exist on the sugar composition of the slime produced, but galactose and glucose have always been identified with galactose predominating in most cases.

show abstract

Section: Homopolysaccharidesmentioning

confidence: 99%

Exocellular polysaccharides produced by lactic acid bacteria

Cerning¹

1990

FEMS Microbiology Letters

429

233

View full text Add to dashboard Cite

show abstract

“…Therefore, the structure of the trisaccharide 111 produced from alternan must be a-D-ghcopyranosyl-1,3-a-D-glUCOpyranosyl-l,6-~-glucose. This trisaccharide is also known to arise as a minor component from the hydrolysis of alternan by isomaltodextranase [26], and has been isolated from acetolyzates of dextran [27] and alternan [28]. Reported values for its optical rotation were +153" at 14°C [27] and +151" at 20°C [28].…”

Section: Monosaccarides Disaccharides and Trisaccharidesmentioning

confidence: 99%

“…arise as a minor component from the hydrolysis of alternan by isomaltodextranase [26], and has been isolated from acetolyzates of dextran [27] and alternan [28]. Reported values for its optical rotation were +153" at 14°C [27] and +151" at 20°C [28].…”

Section: Monosaccarides Disaccharides and Trisaccharidesmentioning

confidence: 99%

Enzymically Produced Cyclic α‐1,3‐Linked and α‐1,6‐Linked Oligosaccharides of d‐Glucose

Côté

Biely

1994

European Journal of Biochemistry

View full text Add to dashboard Cite

A new type of bacterial enzyme hydrolyzed alternan (Leuconostoc mesenteroides NRRL B-1355 fraction S dextran, an alternating a-l,3-a-1,6-D-glucan) to give rise to a series of oligosaccharides. The oligosaccharide formed in the greatest proportion was a cyclic tetrasaccharide of D-glucosyl residues linked in an alternating a-1,3-a-1,6 fashion. Other saccharide products included isomaltose and a-D-glucopyranosyl-1,3-a-D-g~UCOp~~~~OS~~-~,6-~-glucose. Oligosaccharides of higher degrees of polymerization were also formed, and included a-D-glucosyhted derivatives of the cyclic tetrasaccharide. This is the first report of a naturally produced cyclic tetrasaccharide.Relatively few examples are known of cyclic oligosaccharides, the best known being the cyclodextrins [l]. Other cyclic oligosaccharides include the p-1,3-p-1,6-linked glucooligosaccharides of Bradyrhizobiurn spp. Alternan, also referred to in the literature as B-1355 fraction S dextran [16], is produced from sucrose by Leuconostoc rnesenteroides NRRL B-1355 [17]. It is composed predominantly of an alternating sequence of a-1,3-linked and a-1,6-linked D-glucose residues, with approximately 10% branching [18]. While studying the specificity of a new class of enzymes that degrade alternan, we noted that some of the Correspondence to G.

show abstract

“…Using ex-glucosidase and neopullulanase, several researchers 8 ,9) produced sugar mixtures containing isomaltose that act as growth factors for bifidobacteria in vitro and in vivo. 10) Sawai et al, [11][12][13][14][15] reported numerously on enzymatic properties of an isomaltodextranase (EC 3.2.1.94) from Arthrobacter globiformis T6; 1) successive release of isomaltose from nonreducing ends of dextran and isomaltooligosaccharides, 2) the substrate specificity of hydrolysis on isomaltosylex(l ~ 2), ex(l ~ 3), ex(l ~4), and ex(l ~6) linkages, and 3) transfer and condensation activities on isomaltose to produce isomaltotetraose. Therefore, applications of the transfer action of isomaltodextranase will be able to produce various transfer products having isomaltosyl residues, some novel.…”

mentioning

confidence: 99%