Hydrolysis of microalgae cell walls for production of reducing sugar and lipid extraction

Fu, Chun; Hung, Tien‐Chieh; Chen, Jing Yi; Su, Chia‐Hung; Wu, Wen Teng

doi:10.1016/j.biortech.2010.06.100

Cited by 238 publications

(87 citation statements)

References 24 publications

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“…A maximum efficiency by enzymatic pretreatment was found to be at 12 h as 32.74% from nitrogen rich cultures and 51.68% under nitrogen starvation condition. The microalgal cell wall is made up of polysaccharide mainly comprising cellulose that can be hydrolyzed by the enzyme, cellulase [26]. From the data it was clear that the oil extraction was found to be increased with increasing pretreatment time with cellulase.…”

Section: Pretreatment Of N Oculata For Oil Extractionmentioning

confidence: 99%

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Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Surendhiran

Mani

2014

ISRN Chemical Engineering

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Microalga is one of the most compelling microbial biomasses for biodiesel production. Various pretreatment processes, namely, enzyme treatment, lysis by acid, ultrasonicator, microwaves, autoclave, and 40% NaCl, for nitrogen replete and depleted algal cultures of Nannochloropsis oculata had been carried out to check the most feasible and effective technique to disrupt cells for procuring lipids, for which concentrations were determined. Fatty acid composition, essential functional groups, and cell disruption were analyzed by GC-MS, FT-IR Spectroscopy, and Nile Red fluorescent microscopy, respectively. The present investigation showed that lipid yield was higher in nitrogen depleted cells than that in normally nourished cells. GC-MS revealed the presence of major fatty acids-palmitic, oleic, stearic, arachidic, lauric, and linoleic acids. Highest efficiency was found when cells were pretreated using acid for 3 h. The lipid content was calculated as 33.18% and 54.26% for nitrogen rich cells and nitrogen starved cells, respectively. This work thus aided in identifying the most eligible pretreatment process to avail lipids from cells, to convert them to eco-friendly and nonpolluting biodiesel.

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Section: Pretreatment Of N Oculata For Oil Extractionmentioning

confidence: 99%

“…Moreover, an alga does not have lignin in its cell wall, which is a major advantage to perform enzymatic hydrolysis of its polysaccharide components [4]. Fu et al [26] had successfully hydrolysed Chlorella sp. using immobilized cellulase with electrospun polyacrylonitrile (PAN) producing reducing sugar.…”

Section: Pretreatment Of N Oculata For Oil Extractionmentioning

confidence: 99%

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Surendhiran

Mani

2014

ISRN Chemical Engineering

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“…The observation indicated that the microalgae cell wall structure became looser and the lipid could therefore be obtained more easily after dilute acid hydrolysis, and the similar results were also reported in other literatures (Harun and Danquah, 2011;Thu et al, 2009). In fact, enzymatic hydrolysis was also adopted to loosen the microalgae cell wall to release the lipid (Fu et al, 2010).…”

Section: Lipid Extraction After Acid Hydrolysismentioning

confidence: 99%

Joint production of biodiesel and bioethanol from filamentous oleaginous microalgae Tribonema sp.

Wang

et al. 2014

Bioresource Technology

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h i g h l i g h t sA sugar and oil-rich Tribonema strain was used for ethanol and biodiesel production. Acidic hydrolysis can efficiently saccharify microalgae biomass and release lipid. Acidic hydrolysis method promoted the transformation of PL to FFA in lipid. Bioethanol yield from hydrolysate is similar to the theoretical yield. t r a c tMaking full use of lipid and carbohydrate in microalgae for joint production of biodiesel and bioethanol may create a potential way to cut the high cost of single biofuel production from microalgae. Compared with conventional unicellular oleaginous microalgae, filamentous microalgae Tribonema sp. is richer in lipid and carbohydrate contents and lower protein content, thus, this study explores the suitability of Tribonema sp. as a substrate for joint production of biodiesel and bioethanol. Acid hydrolysis is the key step to saccharify wall cell into fermentable sugar and release lipid. Microalgae biomass (50 g/L) was acid (3% H 2 SO 4 ) hydrolyzed at 121°C for 45 min to reach the maximum hydrolysis efficiency (81.48%). Subsequently, the lipid separated with hexane-ethanol from the hydrolysate was converted into microalgae biodiesel and the conversion rate was 98.47%. With yeast Saccharomyces cerevisiae, the maximum ethanol yield of 56.1% was reached from 14.5 g/L glucose in hydrolysate.

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“…Fungal cells contain numerous types of polysaccharide, for instance, chitin, on their cell biomass and have well-known capability of moisture preservation, and co-culturing with fungi cells may increase the difficulty for the subsequent drying of algae biomass [82]. Research finding was published on use of external cellulase on the microalgae biomass in order to hydrolyze the cell wall and facilitate the lipid extraction, therefore, it is possible that the lipid extraction may be improved with co-culture of enzyme producing fungi [83,84]. The lipid extraction from fungal cells may be very different the one from algae cells due to the different cell compositions, therefore, it may complicate the overall lipid extraction process also.…”

Section: Impact On Subsequent Downstreaming Processing Of Microalgae mentioning

confidence: 99%

Review of Microalgae Harvesting via Co-Pelletization with Filamentous Fungus

Gultom

2013

Energies

143

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Cultivation of microalgae to utilize CO 2 and nutrients in the wastewater to generate biofuel products is a promising research objective. However, the process faces tremendous technical difficulties, especially the harvest of microalgae cells, an economically challenging step. Several researchers recently reported co-culturing of filamentous fungi with microalgae so that microalgae cells can be co-pelletized in order to facilitate the cell harvest. This algae pelletization via the filamentous fungi represents an innovative approach to address both the cost and sustainability issues in algae biofuel production and also has potential with direct commercial applications. This paper reviews the current research status in this area and some possible drawbacks of this method in order to provide some possible directions for the future research.

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Hydrolysis of microalgae cell walls for production of reducing sugar and lipid extraction

Cited by 238 publications

References 24 publications

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Joint production of biodiesel and bioethanol from filamentous oleaginous microalgae Tribonema sp.

Review of Microalgae Harvesting via Co-Pelletization with Filamentous Fungus

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