Hydrolysis of Protein and Model Dipeptide Substrates by Attached and Nonattached Marine
            <i>Pseudomonas</i>
            sp. Strain NCIMB 2021

Griffith, P. C.; Fletcher, Madilyn

doi:10.1128/aem.57.8.2186-2191.1991

Cited by 30 publications

(15 citation statements)

References 27 publications

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“…Previous studies have shown that when cells attach to a surface they undergo physiological and metabolic changes ( Davies & McFeters 1988; McFeters et al . 1990 ; Griffith & Fletcher 1991; Davies, Chakrabarty & Geesey 1993; Vandevivere & Kirchman 1993; Ascon‐Cabrera, Ascon‐Reyes & Lebeault 1995; Wentland et al . 1996 ).…”

Section: Discussionmentioning

confidence: 99%

Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine

Cochran¹,

McFeters²,

Stewart

2001

Journal of Applied Microbiology

164

116

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W. L. COCHRAN, G. A. McFETERS and P. S. STEWART.2000. Pseudomonas aeruginosa attached to alginate gel beads in sparse, thin biofilms exhibited reduced susceptibility to monochloramine and hydrogen peroxide compared with planktonic cells of the same micro‐organism. Disinfection rate coefficients for planktonic bacteria averaged 0·55 l mg−1 min−1 for monochloramine and 3·1 × 10−4 l mg−1 min−1 for hydrogen peroxide. The corresponding values for 24‐h‐old biofilm cells were 0·29 l mg min−1 and 9·2 × 10−5 l mg−1 min−1 for monochloramine and hydrogen peroxide, respectively. Several pieces of evidence support the interpretation that the reduced susceptibility of biofilm was not due simply to inadequate delivery of the antimicrobial agent to the local environment of the attached cells. No correlation between biofilm susceptibility and biofilm initial areal cell density was observed. Rapid delivery of hydrogen peroxide to the attachment surface, and subsequently to the interior, of the alginate gel beads was visualized by a direct experimental technique. Theoretical analysis of unsteady diffusion and diffusion–reaction interactions also argued against any significant delay or barrier to antimicrobial or oxygen delivery. It was hypothesized that new genes are expressed when bacteria attach to a surface and begin to form a biofilm and that some of the resulting gene products reduce the susceptibility of the cell to antimicrobial agents including oxidative biocides such as monochloramine and hydrogen peroxide.

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Section: Discussionmentioning

confidence: 99%

Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine

Cochran¹,

McFeters²,

Stewart

2001

Journal of Applied Microbiology

164

116

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“…Enhanced as well as diminished rates of hydrolysis, remineralization, and bacterial growth have been reported for OM bound to seston, sediments, sediment surrogates, and soils (e.g. Dashman and Stotsky 1986;Griffith and Fletcher 199 1;Smith et al 1992;Mayer 1994). Whether sorbed OM is degraded or preserved will depend on the age, location, and structure of the material.…”

mentioning

confidence: 99%

Microbial degradation of sorbed and dissolved protein in seawater

Taylor

1995

Limnol. Oceanogr.

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The effects of adsorption and concentration on rates of protein degradation by marine bacteria were examined by measuring hydrolysis and remineralization of the radiolabeled protein, ribulose-1 +bisphos-phate carboxylase-oxygenase ([meth$3H]Rubisco). Protein adsorbed at low surface concentrations on glass beads had hydrolysis and remineralization rate constants 247% and 282% higher than protein bound at high surface concentrations. Moreover, thin films of bound protein were hydrolyzed and remineralized 522% and 1,033% faster than comparable pool sizes of dissolved protein. At high concentrations, however, rates of' hydrolysis and remineralization were not significantly different between proteins in dissolved or sorbed pools. Results demonstrate that proteins adsorbed to surfaces can be as bioavailable as those in solution. In fact, the sorption process may actually aid bacterial degradation by concentrating the protein and hydrolysates at an interface and by unfolding proteins to relax steric hindrance and expose a higher proportion of bonds to bacterial endo-and exohydrolases.

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“…The interactions between attached bacteria and adsorbed nutrients have been well documented [1, 2, 23–33], but the results of those studies give an inconsistent picture of what occurs when both bacteria and nutrient become immobilised at a solid–liquid surface. In some cases the adsorption of nutrients resulted in recalcitrance of an otherwise readily biodegradable substrate [26, 31], whereas the study of Griffith and Fletcher [2] reported that attached bacteria benefited from nutrients adsorbed to a common surface.…”

Section: Discussionmentioning

confidence: 99%

“…Among the molecules which would be expected to have a higher chemical activity as a result of adsorption are the amphipathic or surface‐active agents, and in 1943 ZoBell [1] reported that bacteria benefited from access to a greater surface area when utilisable surfactants were present. The work of Griffith and Fletcher [2] on the hydrolysis of a low molecular weight dipeptide methylcoumarinylamide leucine (MCAL) and bovine serum albumin (BSA) by attached and non‐attached bacteria has shown the same phenomenon, in that BSA adsorbed to a test adsorbent much more than did MCAL, and was hydrolysed four times faster by bacteria which were attached compared with non‐attached cells. This observation enforces the view that attached bacteria may derive an advantage from being attached when they are metabolising molecules that adsorb to the surface.…”

Section: Introductionmentioning

confidence: 99%

Effect of river sediment on the biodegradation kinetics of surfactant and non-surfactant compounds

Marchesi

White

Russell

et al. 2006

FEMS Microbiology Ecology

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The half‐time of biodegradation of the surfactant sodium dodecylsulfate (SDS) by Pseudomonas species C12B, was reduced two fold by the presence of a riverine sediment. The sediment alone gave comparatively negligible biodegradation of SDS under otherwise equivalent conditions. The sediment had no effect on the kinetics of biodegradation of the non‐surfactant pyruvate by Pseudomonas strain C12B. In the light of (i) the known strong adsorption of the surfactant SDS, but not pyruvate, to river sediment and (ii) the SDS‐stimulated attachment of Pseudomonas C12B and other SDS‐degraders to the sediment, the above observations indicate that acceleration of biodegradation was the result of simultaneous attachment of both SDS and biodegradation competent bacteria to the sediment. This interpretation was strengthened by analysis of the biodegradation kinetics. The data obtained for biodegradation in the absence of sediment, were fitted best by a model involving logistic growth on the added surfactant. The biodegradation data in the presence of sediment were fitted to variants of this model, including one allowing growth on material endogenous to the sediment. Of the several models tested, the data were fitted best by one which is consistent with accumulation of both bacterial cells and substrate at the sediment surface. The enhancement of surfactant biodegradation by sediment is discussed in the context of the design of biodegradability tests and environmental acceptability.

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Hydrolysis of Protein and Model Dipeptide Substrates by Attached and Nonattached Marine Pseudomonas sp. Strain NCIMB 2021

Cited by 30 publications

References 27 publications

Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine

Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine

Microbial degradation of sorbed and dissolved protein in seawater

Effect of river sediment on the biodegradation kinetics of surfactant and non-surfactant compounds

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