Hydrolysis of vegetable and microbial oils catalyzed by a solid preparation of castor bean lipase

Machado, Sara Aparecida; Rós, Patrícia Caroline Molgero Da; Castro, Heizir F. de; Giordani, Domingos S.

doi:10.1016/j.bcab.2021.102188

Cited by 10 publications

(2 citation statements)

References 25 publications

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“…Candida rugosa lipase, Candida antarctica lipase B and Thermomyces lanuginose lipase were found to have the same selective regularity [72]. While some lipases were demonstrated to favor the hydrolysis of natural oils rich in polyunsaturated fatty acids [73,74]. The selectivity of lipases for fatty acids with the same carbon chain length but different unsaturation degree has also been explored.…”

Section: Substrate Specificity Of Lipasementioning

confidence: 99%

Progress and perspectives of enzymatic preparation of human milk fat substitutes

Liu

Dai

Liu

2022

Biotechnol Biofuels

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Human milk fat substitutes (HMFS) with triacylglycerol profiles highly similar to those of human milk fat (HMF) play a crucial role in ensuring the supply in infant nutrition. The synthesis of HMFS as the source of lipids in infant formula has been drawing increasing interest in recent years, since the rate of breastfeeding is getting lower. Due to the mild reaction conditions and the exceptionally high selectivity of enzymes, lipase-mediated HMFS preparation is preferred over chemical catalysis especially for the production of lipids with desired nutritional and functional properties. In this article, recent researches regarding enzymatic production of HMFS are reviewed and specific attention is paid to different enzymatic synthetic route, such as one-step strategy, two-step catalysis and multi-step processes. The key factors influencing enzymatic preparation of HMFS including the specificities of lipase, acyl migration as well as solvent and water activity are presented. This review also highlights the challenges and opportunities for further development of HMFS through enzyme-mediated acylation reactions.

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Section: Substrate Specificity Of Lipasementioning

confidence: 99%

Progress and perspectives of enzymatic preparation of human milk fat substitutes

Liu

Dai

Liu

2022

Biotechnol Biofuels

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“…Nevertheless, owing to the harshness of such alkaline conditions, up to 60% of original γ-oryzanol loss is found in this process (Das et al, 1998;Kaewboonnum et al, 2010;Meedam et al, 2020). An appealing alternative for hydrolysis of glycerides involves using a lipase enzyme known to selectively bind with and cleave the ester bonds in fats and oils and has been examined for the production of FFAs from various sources of glycerides, such as macaw kernel and pulp oils, microalgal oil (Machado, Rós, Castro, & Giordani, 2021), and fish oil (A. . Enzymatic processes have also recently been actively investigated for deacidification of crude RBO through various reactions, such as amidation (X.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic pretreatment of rice bran acid oil before ?-oryzanol recovery

Yahya

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This study aims to apply enzymatic reactions for pretreating the major byproduct of rice bran oil (RBO) industry, rice bran acid oil (RBAO) before ?-oryzanol recovery. RBAO contains free fatty acids (FFAs), glycerides, and is rich in the super-antioxidant, ?-oryzanol. As a primary step to recover ?-oryzanol from RBAO, glycerides must be removed because of having similar polarity with ?-oryzanol. This study is conducted in two parts, hydrolysis and esterification/transesterification. In the first part, enzymatic hydrolysis. The study evaluates the performance of operating conditions using one factor at a time (OFAT) on the reaction time, temperature, lipase loading, water:RBAO ratio, and speed where glycerides removal and ?-oryzanol loss are measured as responses. From these, Face-centered central composite rotatable design (FCCD) was used to investigate the effects of three independent variables: time, temperature, and water:RBAO ratio, and their interactions on the responses: glyceride removal, ?-oryzanol loss, and FFAs production, and to determine the statistical models describing their relationships. In addition, by applying the desirability function approach, the optimal location was identified, corresponding to maximal glyceride removal with an imposed upper limit of 35% ?-oryzanol loss. Lastly, the last section is the confirmation of the validity of the model and at an established optimal condition of 22 h, 48.5 ?C and 1:1 water:RBAO ratio, confirmed the validity of the models: glyceride removal approached completion (99%), ?-oryzanol loss was as low as 32%, and the FFAs production was 7375%. Then the second part is enzymatic esterification/transesterification. First is to evaluate the performance of operating conditions using OFAT of ethanol to RBAO molar ratio, temperature, time of reaction, lipase loading, and speed on the glycerides removal, Fatty acid ethyl ester (FAEE/biodiesel), ?-oryzanol loss, and FFAs remaining. Two optimal conditions at 40?C, 3:1 and 5:1 mol ratio of ethanol to RBAO,10% of lipase loading,200 rpm, and 18 and 24 h reaction time, respectively with >98% glyceride removal. Then, the extraction of ?-oryzanol from FAEE using aqueous ethanolic NaOH is conducted and resultant that around 90% of ?-oryzanol can be extracted at 2M. As a primary step to recover ?-oryzanol from RBAO, glycerides must be removed by conversion into more easily separable components such as FFAs or FAEE. The results of this study demonstrated that enzymatic hydrolysis and esterification/transesterification are promising methods for glyceride removal prior to recovery of ?-oryzanol from RBAO and that the statistical models gave accurate predictions of responses and would be useful for further industrial design of the process also is environmentally friendly biocatalysts. Further modifications in the process may be needed to reduce the content of glycerides. Moreover, the losses of ?-oryzanol from these two methods are much better than the conventional method and are interesting to explore further.�

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Sustainable Castor Bean Biodiesel Through Ricinus communis L. Lipase Extract Catalysis

Rivas

Vázquez

Flores

et al. 2022

Appl Biochem Biotechnol

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Hydrolysis of vegetable and microbial oils catalyzed by a solid preparation of castor bean lipase

Cited by 10 publications

References 25 publications

Progress and perspectives of enzymatic preparation of human milk fat substitutes

Progress and perspectives of enzymatic preparation of human milk fat substitutes

Enzymatic pretreatment of rice bran acid oil before ?-oryzanol recovery

Sustainable Castor Bean Biodiesel Through Ricinus communis L. Lipase Extract Catalysis

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