Hydrolysis of β-lactoglobulin by trypsin under acidic pH and analysis of the hydrolysates with MALDI–TOF–MS/MS

Influence of denaturation and aggregation of β-lactoglobulin on its tryptic hydrolysis and the release of functional peptides

“…Values are expressed as mean ± standard deviation (n = 3). Leeb, & Kulozik, 2011;Creamer, Bienvenue, et al, 2004;Fernández & Riera, 2013). The release of the ACE-inhibitory peptide f(9-14) was modified as a result of prior denaturation of b-LG resulting in conformational changes of the molecule.…”

“…Because of its position in the outer region of the molecule it seems to be preferably accessible for the enzyme. Since the affected peptide bonds in the native protein can easily be accessed by the enzyme, the peptide is directly associated with the enzymatic degradation of the b-LG molecule (Cheison et al, 2011;Fernández & Riera, 2013).…”

Enzymatic generation of whey protein hydrolysates under pH-controlled and non pH-controlled conditions: Impact on physicochemical and bioactive properties

“…As a result, this may affect its hydrolytic activity 68 (Whitehurst, et al, 2002). For instance, hydrolysis of β-lactoglobulin by trypsin at optimal 69 pH (pH 7.8) and at lower pHs showed few differences in the generation of peptides and also 70 in the kinetics of peptide release (Cheison, Lai, Leeb & Kulozik, 2011). It was also shown 71 that the extent of whey protein isolate hydrolysis with trypsin was dependent on the pH 72 oxidative status may occur in type-2 diabetic patients, the antioxidant capacity of the 91 hydrolysates generated was analysed (Rani & Mythili, 2014 …”

Advanced Mass Spectrometry

“…Proteomic approach to detect powdered milk in fresh cow's milk [114] MALDI-TOF-TOF Proteins as targets of ferrous-catalyzed oxidation: identification of protein carbonyls [37] LC-TOF Evaluation of the immunostimulating activity of soybeans via the determination of the functional peptide soymetide [115] SELDI-TOF Effect of NaCl and sucrose tastants on protein composition of oral fluid [44] MALDI-TOF Hydrolysis of β-lactoglobulin by trypsin under acidic pH [116] UHPLC-TOF Quantification of Se-methylselenocysteine and its γ-glutamyl derivative from naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) [50] MALDI-TOF Detection of sheep and goat milk adulterations by direct MALDI-TOF-MS analysis of milk tryptic digests [117] CE-TOF Identification of bioactive peptides in hypoallergenic infant milk formulas [58] UHPLC-QqTOF Differentiation bovine and porcine gelatins in food products [118] [120]…”