Geographically isolated wetlands (GIWs) play a critical role in regional hydrology, streamflow, groundwater recharge, evapotranspiration, and water quality. Despite their importance, research on quantifying discharges from these wetlands remains scarce. This study focuses on the hydrological characterization of GIWs in W-C Florida, a region where they cover a significant proportion of the land surface. The paper introduces a new procedure for developing Stage–Storage–Discharge (rating) characteristics essential for deterministic hydrological modeling in larger geographic areas. The approach synthesizes and extends previous research methodologies and offers simplified key modeling coefficients (m and n), which act as intelligent calibration knobs. These coefficients, when coupled with easily derivable physical attributes such as areas, slopes, and elevations, allow for the accurate simulation of downstream discharge timings and magnitudes, including flood behavior. The proposed method was tested using observational data from well-calibrated models. The results indicated a relative error of −7.2% for stage–storage and 15% for stage–discharge. GIS-based techniques were evaluated against surveyed data, and the results showed an accuracy of 0.16 m (0.52 ft) in estimating both the invert elevation and the maximum depth of GIWs. This effort will ultimately contribute to a better understanding and management of these critical ecosystems.