Hydrophilic and Insoluble Electrospun Cellulose Acetate Fiber-Based Biosensing Platform for 25-Hydroxy Vitamin-D₃ Detection

Abstract

The present work describes the fabrication of a simple, low-cost, eco-friendly, and disposable conducting paper substrate (RCP) decorated with electrospun cellulose acetate fibers (CAEFs) for biosensor application. CAEFs were electrospun under spinning conditions at a voltage of 16 kV, flow rate of 0.2 mL h–1, and tip-to-collector distance of 15 cm. CAEFs were directly collected onto RCP. A scanning electron microscopy study revealed the diameter of CAEFs in the range of 330 ± 3.50 nm. The contact angle of CAEFs was found to be 38.07 ± 5.10°, which indicates a hydrophilic nature of the fibers. Further, the CAEF/RCP electrode was modified with antibody specific to 25-hydroxy vitamin-D3 (AB-25OHD3), followed with bovine serum albumin (BSA) to block nonspecific binding sites for detection of 25-OH vitamin-D3. The BSA/AB-25OHD3/CAEF/RCP immunoelectrode fabrication was confirmed thorough scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and electrochemical techniques. The chronoamperometry response study of the BSA/AB-25OHD3/CAEF/RCP immunoelectrode toward 25-OHD3 detection showed the lowest detection of 10.0 ng mL–1, sensitivity of 0.16 μA ng–1 mL cm–2, and the linear range of 10–100 ng mL–1. This immunoelectrode did not show any interference effect resulted in the higher selectivity for 25-OHD3. The concentration of 25-OHD3 obtained from ELISA of the serum samples collected from the healthy persons showed good agreement with results of validation studies conducted on the BSA/AB-25OHD3/CAEF/RCP immunoelectrode.

Reduced Graphene Oxide‐Gold Nanoparticle Nanohybrid Modified Cost‐Effective Paper‐Based Biosensor for Procalcitonin Detection

“…When PCT concentrations were applied to BSA/anti-PCT/rGO-AuNP@CF bioelectrodes, the current response increased as the PCT concentrations increased. [64] Figure 3 b represents logarithmic calibration plots which displays a linearity in between concentration of PCT and the current with the regression coefficient (R 2 = 0.978) and the limit of detection observed to be 10 pg mL À 1 . Two linear fit has been shown in the ranges 10 to 30 pg mL À 1 and 40 to 15 × 10 3 pg mL À 1, respectively.…”

Electrospun Nanofibers for Sensing and Biosensing Applications—A Review

“…The use of cellulose acetate NFs allowed the detection of 25-hydroxy vitamin D 3 [52]. CA NFs were electrospun onto a conducting paper substrate, which served as an electrode.…”

Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

“…It is due to the reason that 25VD 3 has a much higher serum concentration (~ 99%) and a longer half-life (about 3 weeks) and is therefore measured mainly during the VD test in clinical diagnosis [ 3 ]. Therefore, human plasma/serum 25VD 3 is widely used as a biomarker [ 4 , 5 ]. The standard physiological range of VD is 30–70 ng mL −1 and a value below 30 ng mL −1 indicates an alarming situation of VD deficiency.…”

“…The result can be achieved rapidly and conveniently, allowing for quick decision-making, thus minimizing the risk of human-to-human transmission. Few reports are available in the literature for the development of VD biosensors including the previous work done in our lab for VD detection [ 4 , 11 – 16 ]. However, the biosensor developed for 25VD 3 is very limited as shown in Table 1 .…”