Hydrophilic Interaction Chromatography

Карцова, Л. А.; Бессонова, Е. А.; Somova, V. D.

doi:10.1134/s1061934819050058

Cited by 27 publications

(10 citation statements)

References 108 publications

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“…Similarly to NPLC, HILIC employs traditional polar stationary phases, but in contrast mobile phases (MP) are similar to those used in RPLC (mixtures of an aqueous buffer with a miscible organic solvent). However, as pointed out in several reviews published in the recent years [5][6][7][8][9][10][11][12][13], retention mechanisms in HILIC are complex and are currently under investigation. Even the nature of electrolytes play a role on retention and selectivity [14][15][16].…”

Section: Hilic and Water Uptake By The Stationary Phasementioning

confidence: 99%

HILIC characterization: Estimation of phase volumes and composition for a zwitterionic column

Redón

Subirats

Rosés

2020

Analytica Chimica Acta

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A methodology for the estimation of the different phase volumes in HILIC is presented. For a ZIC-HILIC column the mobile phase volume (hold-up volume) is determined in several acetonitrile-and methanol-water compositions by a Linear Free Energy Relationships (LFER) homologous series approach involving n-alkyl-benzenes, -phenones, and -ketones. We demonstrate that the column works as a HILIC column when the mobile phase contains high and medium proportions of methanol or acetonitrile. However, for acetonitrile contents below 20%, or 40% for methanol, same column works in RPLC. In between, a mixed HILIC-RPLC behavior is observed, and solutes of low molecular volume are retained as in HILIC mode, but the largest ones show RPLC retention. From the homologous series retention data and pycnometric measurements involving the pure organic solvents and their mixtures with water, the mean solvent composition of the water-rich transition layers between column functionalization and the bulk mobile phase, which act as stationary phase, is estimated. Finally, the phase ratio between stationary and mobile phases is also estimated for each eluent composition, allowing the calculation of the corresponding stationary phase volumes. All volumes are strongly dependent on the water content in the eluent, especially when acetonitrile is selected as mobile phase constituent. In HILIC mode, when the water content in the hydroorganic mobile phase increases, the volumes of mobile phase decrease, but the volumes of stationary phase (mainly the water layer adsorbed onto the bonded-phase and the water-enriched interface) increase. However, at high water concentrations, where the column works in RPLC mode, the mobile phase volume increases and the stationary phase (which is now the bonded zwitterion) decrease when increasing the water percentage in the mobile phase.

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Section: Hilic and Water Uptake By The Stationary Phasementioning

confidence: 99%

HILIC characterization: Estimation of phase volumes and composition for a zwitterionic column

Redón

Subirats

Rosés

2020

Analytica Chimica Acta

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“…With the potential of HILIC for the separations of highly polar and ionic compounds, the number of applications of HILIC has recently grown [126,127]. Since the actual retention mechanism of HILIC is not yet completely understood, there have been many published reviews in recent years that attempt to describe the interactions occurring in the column [111,128–130].…”

Section: Methodsmentioning

confidence: 99%

Recent applications of retention modelling in liquid chromatography

Uijl

Schoenmakers

Pirok

et al. 2020

J of Separation Science

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Recent applications of retention modelling in liquid chromatography (2015–2020) are comprehensively reviewed. The fundamentals of the field, which date back much longer, are summarized. Retention modeling is used in retention‐mechanism studies, for determining physical parameters, such as lipophilicity, and for various more‐practical purposes, including method development and optimization, method transfer, and stationary‐phase characterization and comparison. The review focusses on the effects of mobile‐phase composition on retention, but other variables and novel models to describe their effects are also considered. The five most‐common models are addressed in detail, i.e. the log‐linear (linear‐solvent‐strength) model, the quadratic model, the log–log (adsorption) model, the mixed‐mode model, and the Neue–Kuss model. Isocratic and gradient‐elution methods are considered for determining model parameters and the evaluation and validation of fitted models is discussed. Strategies in which retention models are applied for developing and optimizing one‐ and two‐dimensional liquid chromatographic separations are discussed. The review culminates in some overall conclusions and several concrete recommendations.

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“…Over the last two decades, a number of HILIC stationary phases have been introduced [2, 13–18]. Among these, the most cited chemistries are unbonded silica, zwitterionic, amide, diol, and amino materials [18].…”

Section: Introductionmentioning

confidence: 99%

Contribution of ionic interactions to stationary phase selectivity in hydrophilic interaction chromatography

Gilár

Berthelette

Walter

2022

J of Separation Science

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We compared the separation selectivities of 19 different hydrophilic interaction chromatography columns. The stationary phases included underivatized silica and hybrid particles, cyano-bonded silica, materials with neutral ligands such as amide, diol, pentahydroxy, and urea, zwitterionic sorbents, and mixed-mode materials with amine functionalities. A set of 77 small molecules was used to evaluate the columns. We visualized the retention behavior of the different columns using retention time correlation plots. The analytes were classified as cations, anions, or neutral based on their estimated charge under the separation conditions. This involved adjusting the dissociation constants of the analytes for the acetonitrile content of the mobile phase and experimentally determining the pH of the mobile phase containing 70% acetonitrile. The retention correlation plots show that the selectivity differences strongly depended on ionic interactions. Comparisons of the neutral stationary phases (e.g., diol vs. amide) showed more similar selectivity than did comparisons of neutral columns versus columns with cation or anion exchange activity (bare silica or amine columns, respectively). The zwitterionic columns did not behave as perfectly neutral. The correlation plots indicated that they exhibited either cation or anion exchange activity, although to a lesser degree than the silica and amine-containing stationary phases.

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Hydrophilic Interaction Chromatography

Cited by 27 publications

References 108 publications

HILIC characterization: Estimation of phase volumes and composition for a zwitterionic column

HILIC characterization: Estimation of phase volumes and composition for a zwitterionic column

Recent applications of retention modelling in liquid chromatography

Contribution of ionic interactions to stationary phase selectivity in hydrophilic interaction chromatography

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