Hydrophilic Interaction Chromatography (HILIC) for Drug Discovery

Standardization of Analytical Methodology and Procedures for Purity Assessment of Small Molecules in Drug Discovery

“…Bearing in mind the wide diversity of analytes present in an organic extract (microbe, plant or marine source) the use of LC-MS base methods with orthogonal stationary and mobile phases will increase the quality of the bioactive substance. A methodology illustrating the advantage of running orthogonal LC-MS methods to confirm the purity and quality of natural bioactive sample was described by our group (35). In this example, a single peak exhibiting a purity level of >97% was detected by reversed-phase LC-MS.…”