Hydrophilic interaction chromatography of nucleotides and their pathway intermediates on titania

Zhou, Ting; Lucy, Charles A.

doi:10.1016/j.chroma.2008.02.027

Cited by 78 publications

(53 citation statements)

References 53 publications

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“…We formerly established that the separation of the nucleosides was faster and more efficient that previous isocratic separation including our previous EFLC study. 17 When comparing the separation of monophosphate nucleotides to other HILIC studies, 22,23,24 the resolution and selectivity obtained with HILIC-EFLC was either comparable or superior. The best and fastest separation of monophosphate nucleotides in HILIC was achieved by Zhou et al 24 in 7 minutes at 1ml/min.…”

Section: Comparison To Other Hilic Separationsmentioning

confidence: 99%

“…17 When comparing the separation of monophosphate nucleotides to other HILIC studies, 22,23,24 the resolution and selectivity obtained with HILIC-EFLC was either comparable or superior. The best and fastest separation of monophosphate nucleotides in HILIC was achieved by Zhou et al 24 in 7 minutes at 1ml/min. As shown in Figure 8, we, here, separate both the nucleosides and monophosphate nucleotides in just under 12 minutes at a similar flow rate.…”

Section: Comparison To Other Hilic Separationsmentioning

confidence: 99%

“…20,21 Only a few HILIC studies on the separation of nucleotides 22,23,24 have been published to date and none of these publications have separated the nucleotides along with their nucleosides.…”

Section: 2mentioning

confidence: 99%

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Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Philibert

Olesik

2011

Journal of Chromatography A

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Hydrophilic Interaction Chromatography (HILIC) is a liquid chromatographic separation mechanism commonly used for polar biological molecules. While nucleotides are very polar analytes, only a few studies have been conducted on their separation using HILIC. Herein, the use of enhanced-fluidity liquid chromatography (EFLC) for the separation of nucleosides and nucleotides under HILIC conditions is investigated. Enhanced-fluidity liquid chromatography involves using common mobile phases with the addition of substantial proportions of a dissolved gas which provides enhanced mobile phase diffusivity and lower viscosity. The impact of varying mobile phase composition: buffer composition, type of base, salt used, salt concentration and mole fraction of CO 2 was studied to provide optimized HILIC separations. Each of these parameters plays a key role in the retention of the analytes, which demonstrates the complexity of the retention mechanism in HILIC. The tailing of phosphorylated compounds was overcome with the use of phosphate buffer and the addition of a strong base; efficiency and peak asymmetry were compared with the addition of either triethylamine (TEA), 1,4-diazabicyclo

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Section: Comparison To Other Hilic Separationsmentioning

confidence: 99%

Section: Comparison To Other Hilic Separationsmentioning

confidence: 99%

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Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Philibert

Olesik

2011

Journal of Chromatography A

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“…Tani et al used titania as the packing material for ion chromatography to separate inorganic anions and cations simultaneously by adjusting the pH of the buffer [3,4]. Recently, titania was taken as an hydrophilic material to separate nucleotides and their pathway intermediates [5] and carboxylates [6] under hydrophilic interaction chromatography (HILIC) mode. The retention property of nucleotides and their pathway intermediates showed both ligand-exchange and HILIC retention mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid‐phase dispersion using titania

Shi

Liang

et al. 2011

J of Separation Science

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Dalbergia odorifera contains high concentrations of flavonoid aglycones and trace flavonoid glycosides. In this study, trace flavonoid glycosides were separated from D. odorifera by titania with matrix solid-phase dispersion (MSPD). Before the MSPD experiment, four standards, including two isoflavone glycosides (genistin and formononetin-8-C-apiosyl (1-6)-glucoside) and their aglycones (genistein and formononetin), were used to compare their retention on a titania column. The effect of acetonitrile concentration and pH on their retention was investigated and a conclusion was drawn that high acetonitrile concentration and pH lead to the greatest difference in the retention of flavonoid as glycosides and aglycones. Besides hydrophilic interaction and ligand-exchange interaction may exist between sugar moiety of flavonoid glycoside and titania, so that flavonoid glycosides have stronger retention than that of aglycones. Based on the chromatographic rule of flavonoid as glycosides and aglycones on the titania column, the MSPD method was optimized to elute high concentration flavonoid aglycones first with 90% acetonitrile and 10% water containing 100 mM ammonium acetate buffer, and then to elute trace flavonoid glycosides with 20% acetonitrile and 80% water containing 1% trifluoroacetate (TFA). Isolated flavonoid glycosides were further analyzed by UPLC-MS/MS, and their fragmentation in MS 2 showed they are C-glycosyl flavonoids.

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“…By using EDTA, no further peak tailing was observed and the previously disappearing peaks were detected in every analysis. A titania column was used to separate nucleotides and their pathway intermediates [21]. Titania's mechanical and pH stability is better than of silica.…”

Section: Liquid Chromatographymentioning

confidence: 99%

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

Somogyi

Horvai

Csala

et al. 2016

Period. Polytech. Chem. Eng.

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Hydrophilic interaction chromatography of nucleotides and their pathway intermediates on titania

Cited by 78 publications

References 53 publications

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid‐phase dispersion using titania

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

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