Hydrophilic Interaction Liquid Chromatography for LC-MS

Hanai, Toshihiko

doi:10.4172/2469-9861.1000123

Cited by 1 publication

(3 citation statements)

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“…The ionized amino group demonstrated hydrogen bonding with toluene at low pH. This is an interaction between amino-group hydrogen and toluene phenyl-group [30]. The example of electron density maps of these complexes are shown in Figure 3.…”

Section: Definition In Liquid Chromatographymentioning

confidence: 98%

“…Among various polar bonded-phases silica gels, the hexylamino-bonded silica gel is stable and can be used in both aqueous hydrophilic and ion-exchange liquid chromatography with high reproducibility. Hexenyl-bonded silica gel is also used in hydrophilic interaction liquid chromatography (HILIC) [27,30,31]. The model compounds are toluene and benzoic acids in molecular and ionized forms.…”

Section: Mihb = Hb (Molecule A) + Hb (Molecule B) -Hb (Molecule a Andmentioning

confidence: 99%

“…Hydrogen bonding and weak electrostatic interaction contribute to the retention in HILIC. Strong electrostatic interaction is dominant in ion-exchange liquid chromatography [30]. Further studies were carried out using model octyl-, hexenyl-, hexylamino, and hexylguanidino-bonded lead trioxide.…”

Section: Mihb = Hb (Molecule A) + Hb (Molecule B) -Hb (Molecule a Andmentioning

confidence: 99%

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Fundamental Properties of Packing Materials for Liquid Chromatography

Hanai¹

2019

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The high performance of chemically-modified silica gel packing materials is based on the utilization of pure silica gels. Earlier silica gels used to be made from inorganic silica; however, nowadays, silica gels are made from organic silanes. The surface smoothness and lack of trace metals of new silica gels permits easy surface modifications (chemical reactions) and improves the reproducibility and stability. Sharpening peak symmetry is based on developing better surface modification methods (silylation). Typical examples can be found in the chromatography of amitriptyline for silanol testing and that of quinizarin for trace metal testing. These test compounds were selected and demonstrated sensitive results in the measurement of trace amounts of either silanol or trace metals. Here, we demonstrate the three-dimensional model chemical structures of bonded-phase silica gels with surface electron density for easy understanding of the molecular interaction sites with analytes. Furthermore, a quantitative explanation of hydrophilic and hydrophobic liquid chromatographies was provided. The synthesis methods of superficially porous silica gels and their modified products were introduced.

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Section: Definition In Liquid Chromatographymentioning

confidence: 98%

Section: Mihb = Hb (Molecule A) + Hb (Molecule B) -Hb (Molecule a Andmentioning

confidence: 99%

Section: Mihb = Hb (Molecule A) + Hb (Molecule B) -Hb (Molecule a Andmentioning

confidence: 99%

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