Hydrophilic interaction liquid chromatography with alcohol as a weak eluent

Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides

“…3,4,5,6,7,8,9 The chromatographic selectivity for compounds using alcohol-based mobile phases differs significantly from that observed with acetonitrile. Also, due to the enhanced polarity of alcohols compared to acetonitrile, the overall retention decreases as well.…”

Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase

“…As an alternative approach to reversed-phase liquid chromatography (RPLC) which is an indispensable and versatile technique for the separation of a wide range of analytes, hydrophilic interaction chromatography (HILIC) plays an important role in separating highly polar and hydrophilic compounds [1][2][3][4][5][6][7][8][9][10], such as oligosaccharides, peptides, nucleosides, polar pharmaceuticals, etc. Substantial separation materials have been designed specially for HILIC [8,11,12].…”

“…doi:10.1016/j.chroma.2011. 10.086 stationary phase [22] (designated Click TE-Cys, in which "Click TE" stands for "thiol-ene" click chemistry and "Cys" represents cysteine). The Click TE-Cys stationary phase belonged to zwitterionic stationary phases, with the surface charge switching slightly from positive to negative between pH 3 and 7.…”

Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) Metabolomics Method for Shotgun Analysis of Human Urine

“…ACN can act as an acceptor of hydrogen bonds and furthermore provides stronger dipole-dipole interactions. Liu et al (2009) suggested that protic polar solvents like methanol can compete with the polar sites on the stationary phase and in turn disturb the formation of the aqueous layer that is essential for the HILIC partition mechanism [17]. Due to the disturbance in the hydrophobic layer formed the retention could be poor as evident from the results as analytes can form hydrogen bonds easily [18].…”