Hydrophilic Scaffolds Containing Extracts of Stryphnodendron adstringens and Abarema cochliacarpa for Wound Healing: In Vivo Proofs of Concept

Abstract

The present work aimed to evaluate the healing effect of hydrophilic polymeric resorbable biomembrane scaffolds containing plant extracts obtained from two different species, both popularly known as Stryphnodendron adstringens or Barbatimão. The hydrogel-based scaffolds were characterized and submitted to biological tests using Wistar rats to evaluate their healing capacity. The wound retraction index and the evaluation of the inflammatory process and tissue collagenization were recorded. The extracts showed antioxidant activity with IC50 between 10 and 20 µg/mL (DPPH assay) and 4–6 mmol Trolox/g (FRAP assay). The extract of Stryphnodendron adstringens (SA) presented gallocatechin, epigallocatechin, and O-methylpigalocatechin, while the extract of Abarema cochliacarpa (AC) presented catechin, dimers of procyanidins, di-O-hydroxide, O-deoxyhexosi-hexoside, and epicatechin. The membranes containing SA extract (GELSA) were more rigid, with a more intense color, but less thick, with a more compact structure and few pores. The membranes containing AC extract (GELAC) presented a mechanical profile like the gelatin membrane (GEL), with greater permeability to water vapor. The GELAC and GELSA membranes showed similar thermal degradation profiles. The wounds treated with the membranes containing the extracts obtained high levels of retraction of the wounds with values around 60% and 80% in three and seven days, respectively. These data indicate that the compounds of both species have promising biological activities in the repair process, showing that the extracts accelerated the healing process due to the lower intensity of the inflammatory reaction and the presence of compounds such as catechin and epigallocatechin.

Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages

“…Scientific reports corroborate the strong wound-healing activity of S. adstringens [7][8][9][10][11] and experimental findings suggest its therapeutic effect on some chronic inflammatory or infectious conditions [12][13][14][15][16][17][18]. These results suggested that S. adstringens had a relevant anti-inflammatory role, involving its ability to modulate bone marrow macrophage polarization showing a reduction in interleukin 6 (IL-6) cytokines.…”

Atividade Biotecnológica Da Espécie Stryphnodendron Adstringens Na Área Da Saúde – Uma Revisão De Literatura

“…No documento intitulado "Hydrophilic Scaffolds Containing Extracts of Stryphnodendron adstringens and Abarema cochliacarpa for Wound Healing: In Vivo Proofs of Concept", descreve o potencial cicatrizante de uma biomembrana de S. adestringens, foram confirmados o potencial dessa espécie em processo cicatricial em um período de três -sete dias, se tornando um organismo vegetal promissor no processo de reparação tecidual (ALVES, et al 2022).…”

UV-polymerizable methacrylated gelatin (GelMA)-based hydrogel containing tannic acids for wound healing

“…This is because thin and delicate type III collagen fibrils are initially deposited in order to provide a three-dimensional scaffold that guides endothelial migration during the early stages of granulation tissue development. Subsequently, these fibers are progressively replaced by thicker type I collagen fibers with greater tensile strength as the scar tissue matures [ 73 ].…”