Hydrophilic surface-modified 3D printed flexible scaffolds with high ceramic particle concentrations for immunopolarization-regulation and bone regeneration

Li, Wenfeng; Xu, Fancheng; Dai, Fang; Tian, Di; Ai, Yufeng; Xu, Zhiyong; He, Chenjiang; Ai, Fanrong; Li, Song

doi:10.1039/d3bm00362k

Cited by 16 publications

(6 citation statements)

References 56 publications

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“…Macrophages play a crucial role in osteo-immunomodulation because of their direct involvement in the initial inflammatory process of new bone formation [ 65 ]. The transition to the M2 phenotype has been demonstrated as crucial factor for the successful integration of implants and bone [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Calcium phosphate coating enhances osteointegration of melt electrowritten scaffold by regulating macrophage polarization

Shi,

Tao,

Yang

et al. 2024

J Nanobiotechnol

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The osteoimmune microenvironment induced by implants plays a significant role in bone regeneration. It is essential to efficiently and timely switch the macrophage phenotype from M1 to M2 for optimal bone healing. This study examined the impact of a calcium phosphate (CaP) coating on the physiochemical properties of highly ordered polycaprolactone (PCL) scaffolds fabricated using melt electrowritten (MEW). Additionally, it investigated the influence of these scaffolds on macrophage polarization and their immunomodulation on osteogenesis. The results revealed that the CaP coated PCL scaffold exhibited a rougher surface topography and higher hydrophilicity in comparison to the PCL scaffold without coating. Besides, the surface morphology of the coating and the release of Ca2+ from the CaP coating were crucial in regulating the transition of macrophages from M1 to M2 phenotypes. They might activate the PI3K/AKT and cAMP-PKA pathways, respectively, to facilitate M2 polarization. In addition, the osteoimmune microenvironment induced by CaP coated PCL could not only enhance the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro but also promote the bone regeneration in vivo. Taken together, the CaP coating can be employed to control the phenotypic switching of macrophages, thereby creating a beneficial immunomodulatory microenvironment that promotes bone regeneration. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Calcium phosphate coating enhances osteointegration of melt electrowritten scaffold by regulating macrophage polarization

Shi,

Tao,

Yang

et al. 2024

J Nanobiotechnol

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“…In 3D-printed high-ceramic content composites of PCL and HAp, alkali surface modification was utilized to enhance the surface hydrophilicity of the PCL/hyaluronic acid scaffold, and regulate the immune responses. Results showed that the modification of scaffold by NaOH solution reduced foreign body reactions considerably and also promoted macrophage polarization towards the M2 phenotype and improved new bone formation [206]. It can be concluded that through using hydrophilic materials, and processes that alter surface groups and increase surface roughness, hydrophilicity can be improved and thus the fate of cells can be changed.…”

Section: Hydrophilicitymentioning

confidence: 98%

“…Surface chemistry plays a critical role in determining the interactions among BTE scaffolds and cells, proteins, and other biological components in the surrounding environment [206]. First, surface chemistry affects cell adhesion and migration.…”

Section: Surface Chemistry and Compositionmentioning

confidence: 99%

“…First, surface chemistry affects cell adhesion and migration. The surface chemistry of scaffolds affects the adhesion and migration of cells vital for bone regeneration, along which are osteoblasts and MSCs [206]. Certain chemical functionalities, which include peptides containing the arginine-glycine-aspartic acids, can promote cellular adhesion by binding to integrin receptors on cell membranes [207].…”

Section: Surface Chemistry and Compositionmentioning

confidence: 99%

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Recent trends in bone tissue engineering: a review of materials, methods, and structures

Moghaddam,

Bahrami,

Mirzadeh

et al. 2024

Biomed. Mater.

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Bone tissue engineering provides the treatment possibility for segmental long bone defects that are currently an orthopedic dilemma. This review explains different strategies, from biological, material, and preparation points of view, such as using different stem cells, ceramics, and metals, and their corresponding properties for bone tissue engineering applications. In addition, factors such as porosity, surface chemistry, hydrophilicity and degradation behavior that affect scaffold success are introduced. Besides, the most widely used production methods that result in porous materials are discussed. Gene delivery and secretome-based therapies are also introduced as a new generation of therapies. This review outlines the positive results and important limitations remaining in the clinical application of novel bone tissue engineering materials and methods for segmental defects.

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“…In contrast to traditional methods that focus on surface functional group engineering and biochemical molecule delivery, simulating the extracellular matrix microenvironment offers a more direct and effective strategy to regulate osteogenesis [ 14 , 15 ]. It has been discovered that altering the hydrophilicity of materials can modulate the behavior of bone marrow mesenchymal stem cells (BMSCs) and promote osteogenic differentiation [ 13 , 16 , 17 ]. Moreover, material stiffness can induce the expression of mechanosensitive proteins, the secretion of pro-angiogenic molecules, and accelerate bone healing [ 11 , 18 – 20 ].…”

Section: Introductionmentioning

confidence: 99%

Injectable ultrasound-powered bone-adhesive nanocomposite hydrogel for electrically accelerated irregular bone defect healing

Zhou,

Xiao,

Fan

et al. 2024

J Nanobiotechnol

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The treatment of critical-size bone defects with irregular shapes remains a major challenge in the field of orthopedics. Bone implants with adaptability to complex morphological bone defects, bone-adhesive properties, and potent osteogenic capacity are necessary. Here, a shape-adaptive, highly bone-adhesive, and ultrasound-powered injectable nanocomposite hydrogel is developed via dynamic covalent crosslinking of amine-modified piezoelectric nanoparticles and biopolymer hydrogel networks for electrically accelerated bone healing. Depending on the inorganic-organic interaction between the amino-modified piezoelectric nanoparticles and the bio-adhesive hydrogel network, the bone adhesive strength of the prepared hydrogel exhibited an approximately 3-fold increase. In response to ultrasound radiation, the nanocomposite hydrogel could generate a controllable electrical output (-41.16 to 61.82 mV) to enhance the osteogenic effect in vitro and in vivo significantly. Rat critical-size calvarial defect repair validates accelerated bone healing. In addition, bioinformatics analysis reveals that the ultrasound-responsive nanocomposite hydrogel enhanced the osteogenic differentiation of bone mesenchymal stem cells by increasing calcium ion influx and up-regulating the PI3K/AKT and MEK/ERK signaling pathways. Overall, the present work reveals a novel wireless ultrasound-powered bone-adhesive nanocomposite hydrogel that broadens the therapeutic horizons for irregular bone defects.

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Hydrophilic surface-modified 3D printed flexible scaffolds with high ceramic particle concentrations for immunopolarization-regulation and bone regeneration

Abstract: Bioceramic scaffolds used in bone tissue engineering suffer from a low concentration of ceramic particles (<50 wt%), because the high concentration of ceramic particles increases the brittleness of the composite....

Cited by 16 publications

References 56 publications

Calcium phosphate coating enhances osteointegration of melt electrowritten scaffold by regulating macrophage polarization

Calcium phosphate coating enhances osteointegration of melt electrowritten scaffold by regulating macrophage polarization

Recent trends in bone tissue engineering: a review of materials, methods, and structures

Injectable ultrasound-powered bone-adhesive nanocomposite hydrogel for electrically accelerated irregular bone defect healing

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