The present study evaluated short-time effect of steam modified biochar with rhamnolipid solution on the removal of different volatile organic and inorganic compounds (acetone, xylene, ammonia) in biofilter with capillary system. Modification of pine wood biochar with steam governed better treatment of pollar acetone and ammonia compounds from the air stream with removal efficiencies of 93% and 96%, respectively. It was related to biochar’s higher average capillary radius, moisture content and lower air velocity. However, removal of xylene was much lower and reached 72% in the case of modified biochar. It can be explained by its higher Henry’s constant, hydrophobicity and non-polarity.