Hydrophobic affinity partition in aqueous two‐phase systems containing polyethylene glycol)‐palmitate of rightside‐out and inside‐out vesicles from human erythrocyte membranes

The separation of cells and organelles by partitioning in two-polymer aqueous phases

“…monopalmitate derivative of poly(ethylene glycol) nd A3 are of (Walter & Krob, 1976 Partition behaviour ofcells and particles The partitioning behaviour of cells and particles between the two aqueous phases and the interface is readily characterized. The number of cells in each phase may be measured and those present at the interface determined by difference from the total number in the svstem.…”

Molecular sieving action of the cell membrane during gradual osmotic hemolysis

“…This conjecture is supported by two independent types of experiments. Tanaka and Ohnishi (1976) interpreted their phosphatidyl choline and phosphatidyl serine spin label data in terms of a more fluid inner than outer membrane leaflet, and Walter and Krob (1976) found that the outer membrane leaflet was more hydrophobic than the inner leaflet using right-side-out and inside-out RBC vesicles in a dextran-polyethylene glycol countercurrent distribution system. Lipids extracted from human RBCls undergo a phase transition over a broad temperature range, which includes the experimental temperatures of this work and normal body temperature (Ladbrooke and Chapman, 1969).…”