Hydrophobic-interaction chromatography for purification of influenza A and B virus

Weigel, Thomas; Soliman, Remon; Wolff, Michael W.; Reichl, Udo

doi:10.1016/j.jchromb.2019.03.037

Cited by 28 publications

(19 citation statements)

References 45 publications

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“…HIC processes for virus purification are less well characterized and applied than the use of anion exchange methods. However, Weigel et al described a resin-based HIC purification for Influenza A with > 90 % virus yield and about 99 % DNA removal ( Weigel et al, 2019 ). Furthermore, also MVA was previously purified with the HICP membranes as a secondary purification, yielding up to 76 % of the virus ( Wolff et al, 2010a ), matching the data of 76 % virus in the elution pool that we obtained ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

Lothert

Pagallies

Feger

et al. 2020

Journal of Biotechnology

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Highlights Estimation of the isoelectric point and size of Vero cell-derived Orf virus. Limited dynamic binding capacity of tested Orf virus to sulfated cellulose. Purification of Orf virus by steric exclusion chromatography lead to 84 % recovery. Hydrophobic interaction chromatography suitable for Orf virus purification. Promising unit operations for a scalable DSP to produce Orf virus viral vectors.

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Section: Discussionmentioning

confidence: 99%

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

Lothert

Pagallies

Feger

et al. 2020

Journal of Biotechnology

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“…Hydrophobic interaction chromatography (HIC) has been used for the purification of vaccinia virus, foot-and-mouth disease virus and influenza virus, although its use is far lower than IEC [89,[98][99][100]. Moreover, the high salt concentrations used in HIC can damage the integrity of the particles, being one of the reasons why this chromatographic technique is less employed [101].…”

Section: Intermediate Purificationconcentration and Chromatographymentioning

confidence: 99%

Current challenges in biotherapeutic particles manufacturing

Moleirinho

Silva

Alves

et al. 2019

Expert Opinion on Biological Therapy

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Introduction: The development of novel complex biotherapeutics led to new challenges in biopharmaceutical industry. The potential of these particles has been demonstrated by the approval of several products, in the different fields of gene therapy, oncolytic therapy, and tumor vaccines. However, their manufacturing still presents challenges related to the high dosages and purity required. Areas covered: The main challenges that biopharmaceutical industry faces today and the most recent developments in the manufacturing of different biotherapeutic particles are reported here. Several unit operations and downstream trains to purify virus, virus-like particles and extracellular vesicles are described. Innovations on the different purification steps are also highlighted with an eye on the implementation of continuous and integrated processes. Expert opinion: Manufacturing platforms that consist of a low number of unit operations, with higheryielding processes and reduced costs will be highly appreciated by the industry. The pipeline of complex therapeutic particles is expanding and there is a clear need for advanced tools and manufacturing capacity. The use of single-use technologies, as well as continuous integrated operations, are gaining ground in the biopharmaceutical industry and should be supported by more accurate and faster analytical methods.

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“…In HIC, at high ionic strength, binding of proteins to ligands occurs whereas, at low ionic strength, elution of proteins occurs (McCue, 2009;Fekete et al, 2016). In a recent study, the influenza A and B viruses were successfully purified by HIC with up to 96% virus recoveries and about 1.3% residual DNA level using a 96-well-plate format (Weigel et al, 2019). Size exclusion chromatography (SEC) or gel filtration chromatography separates protein molecules based on their molecular weight.…”

Section: Chromatography Processesmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

402

243

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Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.

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Hydrophobic-interaction chromatography for purification of influenza A and B virus

Cited by 28 publications

References 45 publications

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

Current challenges in biotherapeutic particles manufacturing

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

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