Hydrophobic Laminin-Related Peptides: Synthesis and Surface Properties

Interfacial Interactions of Hydrophobic Peptides with Lipid Bilayers

“…This results in a slightly more evident disturbance promoted by C 10 than by C 14 . This apparent stronger activity of decanoyl peptide has already been observed in other physicochemical experiments already described (12).…”

“…Details about synthetic protocols and chemical characterization are given in (12) and their chemical structures are given above. Liposomal composition was based on phosphatidylcholine because this lipid is the main component of cell membranes.…”

“…Details of the synthesis and characterization are given elsewhere (12). Peptide concentration was quantified by amino acid analysis using Nle as internal standard.…”

“…We previously found (12) that these hydrophobic derivatives of the parent sequence YIGSR 2 behave as amphiphilic compounds and are able to spread on water surfaces forming monomolecular layers and 2 Abbreviations used: Y, tyrosine; I, isoleucine; G, glycine; S, serine; R, arginine; DSC, differential scanning calorimetry; CF, carboxyfluorescein; DPPC, dipalmitoylphosphatidylcholine; NBD-PE, N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl) phosphatidylethanolamine; Rh-PE, L-α-phosphatidylethanolamine-Nlissamine-rhodamine B sulfonyl); TLC, thin-layer chromatography; Nle, norleucine; DMSO, dimethyl sulfoxide; PL phospholipid; DPH, 1, 6-diphenyl-1,3,5-hexatriene; TMA-DPH, trimethylammonium 1,6-diphenyl-1,3,5-hexatriene; ANS, 1-anilinonaphthalene-8-sulfonate; NGPE, N -glutaryl phosphatidylethanolamine; DSPE-PEG, diestearoylphosphatidylethanolamine polyethylene glycol; DSPE-PEG-COOH diestearoylphosphatidylethanoaminepolyethylene glycol-carboxylate; MES, 2-(N -morpholino)ethanesulfonic acid; MLV, multilamelar vesicles; EDC, N -(3-dimethylaminopropyl)-Nethylcarbodiimide; NHSS, N -hydroxysulfosuccinimide.…”

Synthesis and study of molecular interactions between phosphatidyl choline and two laminin derived peptides hydrophobically modified

“…Tyrosine was included to better detect peptides by UV methods. Moreover, the presence of a hydrophobic moiety (myristoyl) in the sequence would help the association of the peptide to the surface of vesicles [14,15].…”